Matches in SemOpenAlex for { <https://semopenalex.org/work/W2609871566> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W2609871566 endingPage "124" @default.
- W2609871566 startingPage "117" @default.
- W2609871566 abstract "Abstract Seaweeds have structurally diverse metabolites with biotechnological importance, including lectins, considered a variable class of proteins which bind reversibly to specific carbohydrates. The Caulerpa cupressoides (Chlorophyta) lectin (CcL) exhibiting in vivo pharmacological actions has been described; however, CcL-based diagnostic tool for cancer has not been investigated. This study analyzed molecular weight, cytotoxicity and the in vitro interaction against human promyelocytic leukemia cell lines (HL-60). Extraction of CcL was performed in 25 mM Tris-HCl buffer (TB) (4 h stirring, pH 7.5) and then CcL was purified by ion-exchange chromatography on DEAE-cellulose column using TB containing 0.5 M NaCl, which eluted protein peak exhibiting haemagglutinating activity against trypsin-treated rabbit erythrocytes. Polyacrilamide gel electrophoresis in the presence of β-mercaptoethanol revealed to be a dimeric protein (17 kDa) by comparison with the markers trypsin inhibitor and α-lactoalbulmin. CcL did not cause cytotoxicity up to 500 μg mL-1 in HL-60 cells using MTT reduction assay; but, showed agglutination (125, 250 and 500 μg mL-1) in comparison to Concanavalin A (50 μg mL-1) based on photomicrographic analysis. The results allow us to consider CcL as a promising diagnostic tool for HL-60 cells in further studies.Resumo As algas marinhas possuem metabolitos diversos estruturalmente com importância biotecnologica, incluindo lectinas, consideradas uma classe variavel de proteinas as quais reversivelmente se ligam a carboidratos especificos. A lectina de Caulerpa cupressoides (Chlorophyta) (LCc) tem mostrado acoes farmacologicas in vivo. Entretanto, nao tem sido investigada como ferramenta diagnostica para o câncer. Analisou-se o peso molecular, citotoxidade e a interacao in vitro com celulas de leucemia promielocitica da linhagem (LH-60). A extracao de LCc foi desenvolvida em tampao Tris-HCl 25 mM (TT) (4 h sob agitacao; pH 7,5) e em seguida, a LCc foi purificada por cromatografia de troca ionica em coluna de DEAE-celulose usando TT contendo NaCl 0,5 M, da qual pico proteico eluido apresentou atividade hemaglutinante contra eritrocitos de coelho tratados com tripsina. A eletroforese em gel de poliacrilamida, na presenca de β-mercaptoetanol, revelou uma proteina dimerica (17 kDa) por comparacao com os marcadores inibidor de tripsina e α-lactoalbumina. A LCc nao mostrou, em ensaio de reducao pelo MTT, citotoxicidade ate 500 μg mL-1 em celulas LH-60. Contudo, na analise fotomicrografica, mostrou aglutinacao (125, 250 e 500 μg mL-1) em comparacao a Concanavalina A (50 μg mL-1). Os resultados permitem a considerar LCc como uma ferramenta diagnostica promissora em estudos futuros realizados com celulas LH-60." @default.
- W2609871566 created "2017-05-05" @default.
- W2609871566 creator A5005005264 @default.
- W2609871566 creator A5007643292 @default.
- W2609871566 creator A5024695370 @default.
- W2609871566 creator A5054926688 @default.
- W2609871566 creator A5073168859 @default.
- W2609871566 date "2016-11-13" @default.
- W2609871566 modified "2023-09-27" @default.
- W2609871566 title "In vitro interaction of the native lectin isolated from the green seaweed Caulerpa cupressoides var. lycopodium (Caulerpaceae, Bryopsidales) against cancer HL-60 cells / Interação in vitro da lectina nativa isolada da alga marinha verde Caulerpa cupressoides var. lycopodium (Caulerpaceae, Bryopsidales) contra células cancerígenas LH-60" @default.
- W2609871566 doi "https://doi.org/10.2312/actafish.2016.4.2.117-124" @default.
- W2609871566 hasPublicationYear "2016" @default.
- W2609871566 type Work @default.
- W2609871566 sameAs 2609871566 @default.
- W2609871566 citedByCount "0" @default.
- W2609871566 crossrefType "journal-article" @default.
- W2609871566 hasAuthorship W2609871566A5005005264 @default.
- W2609871566 hasAuthorship W2609871566A5007643292 @default.
- W2609871566 hasAuthorship W2609871566A5024695370 @default.
- W2609871566 hasAuthorship W2609871566A5054926688 @default.
- W2609871566 hasAuthorship W2609871566A5073168859 @default.
- W2609871566 hasConcept C109316439 @default.
- W2609871566 hasConcept C150903083 @default.
- W2609871566 hasConcept C153911025 @default.
- W2609871566 hasConcept C181199279 @default.
- W2609871566 hasConcept C185592680 @default.
- W2609871566 hasConcept C202751555 @default.
- W2609871566 hasConcept C207001950 @default.
- W2609871566 hasConcept C2777563530 @default.
- W2609871566 hasConcept C2780340462 @default.
- W2609871566 hasConcept C2780758401 @default.
- W2609871566 hasConcept C55493867 @default.
- W2609871566 hasConcept C559758991 @default.
- W2609871566 hasConcept C59822182 @default.
- W2609871566 hasConcept C86803240 @default.
- W2609871566 hasConceptScore W2609871566C109316439 @default.
- W2609871566 hasConceptScore W2609871566C150903083 @default.
- W2609871566 hasConceptScore W2609871566C153911025 @default.
- W2609871566 hasConceptScore W2609871566C181199279 @default.
- W2609871566 hasConceptScore W2609871566C185592680 @default.
- W2609871566 hasConceptScore W2609871566C202751555 @default.
- W2609871566 hasConceptScore W2609871566C207001950 @default.
- W2609871566 hasConceptScore W2609871566C2777563530 @default.
- W2609871566 hasConceptScore W2609871566C2780340462 @default.
- W2609871566 hasConceptScore W2609871566C2780758401 @default.
- W2609871566 hasConceptScore W2609871566C55493867 @default.
- W2609871566 hasConceptScore W2609871566C559758991 @default.
- W2609871566 hasConceptScore W2609871566C59822182 @default.
- W2609871566 hasConceptScore W2609871566C86803240 @default.
- W2609871566 hasIssue "2" @default.
- W2609871566 hasLocation W26098715661 @default.
- W2609871566 hasOpenAccess W2609871566 @default.
- W2609871566 hasPrimaryLocation W26098715661 @default.
- W2609871566 hasVolume "4" @default.
- W2609871566 isParatext "false" @default.
- W2609871566 isRetracted "false" @default.
- W2609871566 magId "2609871566" @default.
- W2609871566 workType "article" @default.