FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2610362971> ?p ?o ?g. }

• W2610362971 abstract "Abstract N-linked glycosylation of proteins has both functional and structural significance. Importantly, the glycan structure of a therapeutic protein influences its efficacy, pharmacokinetics, pharmacodynamics and immunogenicity. In this work, we developed glycosylation flux analysis (GFA) for predicting intracellular production and consumption rates (fluxes) of glycoforms, and applied this method to CHO fed-batch monoclonal antibody (mAb) production using two different media compositions, with and without additional manganese feeding. The GFA is based on a constraint-based modelling of the glycosylation network, employing a pseudo steady state assumption. While the glycosylation fluxes in the network are balanced at each time point, the GFA allows the fluxes to vary with time by way of two scaling factors: (1) an enzyme-specific factor that captures the temporal changes among glycosylation reactions catalyzed by the same enzyme, and (2) the cell specific productivity factor that accounts for the dynamic changes in the mAb production rate. The GFA of the CHO fed-batch cultivations showed that regardless of the media composition, the fluxes of galactosylation decreased with the cultivation time in comparison to the other glycosylation reactions. Furthermore, the GFA showed that the addition of Mn, a cofactor of galactosyltransferase, has the effect of increasing the galactosylation fluxes but only during the beginning of the cultivation period. The results thus demonstrated the power of the GFA in delineating the dynamic alterations of the glycosylation fluxes by local (enzyme-specific) and global (cell specific productivity) factors." @default.
• W2610362971 created "2017-05-12" @default.
• W2610362971 creator A5004881005 @default.
• W2610362971 creator A5011454541 @default.
• W2610362971 creator A5031614761 @default.
• W2610362971 creator A5054672110 @default.
• W2610362971 creator A5065890830 @default.
• W2610362971 creator A5077814051 @default.
• W2610362971 creator A5091439592 @default.
• W2610362971 date "2017-03-28" @default.
• W2610362971 modified "2023-09-27" @default.
• W2610362971 title "Glycosylation flux analysis reveals dynamic changes of intracellular glycosylation flux distribution in Chinese hamster ovary fed-batch cultures" @default.
• W2610362971 cites W136234913 @default.
• W2610362971 cites W142220879 @default.
• W2610362971 cites W1518819083 @default.
• W2610362971 cites W1570057002 @default.
• W2610362971 cites W1964389518 @default.
• W2610362971 cites W1972375269 @default.
• W2610362971 cites W1989801492 @default.
• W2610362971 cites W1990260057 @default.
• W2610362971 cites W1992083110 @default.
• W2610362971 cites W2014720377 @default.
• W2610362971 cites W2018533661 @default.
• W2610362971 cites W2021861438 @default.
• W2610362971 cites W2023106695 @default.
• W2610362971 cites W2035530007 @default.
• W2610362971 cites W2037656934 @default.
• W2610362971 cites W2041430035 @default.
• W2610362971 cites W2044935306 @default.
• W2610362971 cites W2045096014 @default.
• W2610362971 cites W2071869547 @default.
• W2610362971 cites W2078355959 @default.
• W2610362971 cites W2081709738 @default.
• W2610362971 cites W2088713628 @default.
• W2610362971 cites W2106091764 @default.
• W2610362971 cites W2109202643 @default.
• W2610362971 cites W2119616127 @default.
• W2610362971 cites W2146981871 @default.
• W2610362971 cites W2147810684 @default.
• W2610362971 cites W2154258517 @default.
• W2610362971 cites W2155196267 @default.
• W2610362971 cites W2160650963 @default.
• W2610362971 cites W2168338758 @default.
• W2610362971 cites W2170416222 @default.
• W2610362971 cites W2184464034 @default.
• W2610362971 cites W2306423776 @default.
• W2610362971 cites W2471543693 @default.
• W2610362971 cites W2742459232 @default.
• W2610362971 doi "https://doi.org/10.1101/121517" @default.
• W2610362971 hasPublicationYear "2017" @default.
• W2610362971 type Work @default.
• W2610362971 sameAs 2610362971 @default.
• W2610362971 citedByCount "0" @default.
• W2610362971 crossrefType "posted-content" @default.
• W2610362971 hasAuthorship W2610362971A5004881005 @default.
• W2610362971 hasAuthorship W2610362971A5011454541 @default.
• W2610362971 hasAuthorship W2610362971A5031614761 @default.
• W2610362971 hasAuthorship W2610362971A5054672110 @default.
• W2610362971 hasAuthorship W2610362971A5065890830 @default.
• W2610362971 hasAuthorship W2610362971A5077814051 @default.
• W2610362971 hasAuthorship W2610362971A5091439592 @default.
• W2610362971 hasBestOaLocation W26103629711 @default.
• W2610362971 hasConcept C159654299 @default.
• W2610362971 hasConcept C170493617 @default.
• W2610362971 hasConcept C175656101 @default.
• W2610362971 hasConcept C178790620 @default.
• W2610362971 hasConcept C185592680 @default.
• W2610362971 hasConcept C2777313579 @default.
• W2610362971 hasConcept C542903549 @default.
• W2610362971 hasConcept C54355233 @default.
• W2610362971 hasConcept C55493867 @default.
• W2610362971 hasConcept C68709404 @default.
• W2610362971 hasConcept C79879829 @default.
• W2610362971 hasConcept C86803240 @default.
• W2610362971 hasConceptScore W2610362971C159654299 @default.
• W2610362971 hasConceptScore W2610362971C170493617 @default.
• W2610362971 hasConceptScore W2610362971C175656101 @default.
• W2610362971 hasConceptScore W2610362971C178790620 @default.
• W2610362971 hasConceptScore W2610362971C185592680 @default.
• W2610362971 hasConceptScore W2610362971C2777313579 @default.
• W2610362971 hasConceptScore W2610362971C542903549 @default.
• W2610362971 hasConceptScore W2610362971C54355233 @default.
• W2610362971 hasConceptScore W2610362971C55493867 @default.
• W2610362971 hasConceptScore W2610362971C68709404 @default.
• W2610362971 hasConceptScore W2610362971C79879829 @default.
• W2610362971 hasConceptScore W2610362971C86803240 @default.
• W2610362971 hasLocation W26103629711 @default.
• W2610362971 hasOpenAccess W2610362971 @default.
• W2610362971 hasPrimaryLocation W26103629711 @default.
• W2610362971 hasRelatedWork W1984939224 @default.
• W2610362971 hasRelatedWork W1990688686 @default.
• W2610362971 hasRelatedWork W2029314177 @default.
• W2610362971 hasRelatedWork W2029321084 @default.
• W2610362971 hasRelatedWork W2128761336 @default.
• W2610362971 hasRelatedWork W2139144356 @default.
• W2610362971 hasRelatedWork W2159423922 @default.
• W2610362971 hasRelatedWork W2608656888 @default.
• W2610362971 hasRelatedWork W3083628667 @default.
• W2610362971 hasRelatedWork W4283009820 @default.
• W2610362971 isParatext "false" @default.

• next