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• W2610476309 abstract "Molecularly imprinted polymers (MIPs) rendered selective solely by the imprinting with protein templates lacking of distinctive properties to facilitate strong target-MIP interaction are likely to exhibit medium to low template binding affinities. While this prohibits the use of such MIPs for applications requiring the assessment of very low template concentrations, their implementation for the quantification of high-abundance proteins seems to have a clear niche in the analytical practice. We investigated this opportunity by developing a polyscopoletin-based MIP nanofilm for the electrochemical determination of elevated human serum albumin (HSA) in urine. As reference for a low abundance protein ferritin-MIPs were also prepared by the same procedure. Under optimal conditions, the imprinted sensors gave a linear response to HSA in the concentration range of 20-100 mg/dm3, and to ferritin in the range of 120-360 mg/dm3. While as expected the obtained limit of detection was not sufficient to determine endogenous ferritin in plasma, the HSA-sensor was successfully employed to analyse urine samples of patients with albuminuria. The results suggest that MIP-based sensors may be applicable for quantifying high abundance proteins in a clinical setting." @default.
• W2610476309 created "2017-05-12" @default.
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• W2610476309 date "2017-07-01" @default.
• W2610476309 modified "2023-10-18" @default.
• W2610476309 title "Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection" @default.
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• W2610476309 doi "https://doi.org/10.1016/j.aca.2017.04.043" @default.
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