FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2610503334> ?p ?o ?g. }

• W2610503334 endingPage "397" @default.
• W2610503334 startingPage "397" @default.
• W2610503334 abstract "Proc Amer Assoc Cancer Res, Volume 45, 20041726 At presentation 25-30% of human bladder tumours are classified as muscle–infiltrative, which by definition have already demonstrated the ability to invade & metastasise. Patients with these tumours have a significantly reduced 5-year survival rate, which often correlates with the development of metastatic disease following failure of treatment such as surgery or radiotherapy. Since metastasis is a multi-step process, in which the tumour cell must overcome many obstacles, it is likely that it involves the de-regulation of many genes. This complexity, combined with limitations of current animal models, means that bladder cancer metastasis is poorly understood. In an effort to further understand the metastatic process, we have established a metastatic variant of a non-metastatic rat bladder cell line. This was achieved by serial orthotopic implantation of the non-metastatic line into the bladder wall of immunocompetant animals. We have compared gene expression between the non-metastatic parent line and the metastatic variant using the Affymetrix rat 230A microarray. The results demonstrated that a total of 248 gene sequences differed by a factor of 2.5 or more between the two cell lines. Of these 248 sequences, 104 were known genes, with 55 being down-regulated in the metastatic cell line compared with the parental line and 49 being up-regulated. Furthermore, 33 of the 55 down-regulated known genes were absent altogether from the metastatic variant, while 22 of the 49 up-regulated known genes were present in the metastatic variant but absent from the parental line. Three classes of gene were strikingly well represented in the panel of differentially expressed genes; these encode proteins involved in (1) proteolysis (e.g. MMP3, MMP10, Cathepsin Y, cystatin N, TIMP3), (2) adhesion or cell-cell communication (e.g. connexins 30 and 40, various integrins) and (3) composition or stability of the extracellular matrix (e.g. fibronectin, decorin, lysyl oxidase, fibulin 3). Expression of candidate genes identified by the microarray experiment has been verified by RT-PCR and western blotting analysis; the pattern of differential protein expression is consistent with that of their mRNAs in the two variants. This model system may be suitable for identifying genes potentially involved in metastasis of bladder cancer and suggest novel targets for therapeutic intervention." @default.
• W2610503334 created "2017-05-12" @default.
• W2610503334 creator A5000275517 @default.
• W2610503334 creator A5042524186 @default.
• W2610503334 creator A5085567931 @default.
• W2610503334 date "2004-04-01" @default.
• W2610503334 modified "2023-09-24" @default.
• W2610503334 title "Identification of differentially expressed genes in a metastatic variant of an orthotopic model of bladder cancer" @default.
• W2610503334 hasPublicationYear "2004" @default.
• W2610503334 type Work @default.
• W2610503334 sameAs 2610503334 @default.
• W2610503334 citedByCount "0" @default.
• W2610503334 crossrefType "journal-article" @default.
• W2610503334 hasAuthorship W2610503334A5000275517 @default.
• W2610503334 hasAuthorship W2610503334A5042524186 @default.
• W2610503334 hasAuthorship W2610503334A5085567931 @default.
• W2610503334 hasConcept C104317684 @default.
• W2610503334 hasConcept C121608353 @default.
• W2610503334 hasConcept C126322002 @default.
• W2610503334 hasConcept C142724271 @default.
• W2610503334 hasConcept C143998085 @default.
• W2610503334 hasConcept C150194340 @default.
• W2610503334 hasConcept C2779013556 @default.
• W2610503334 hasConcept C2780352672 @default.
• W2610503334 hasConcept C502942594 @default.
• W2610503334 hasConcept C54355233 @default.
• W2610503334 hasConcept C71924100 @default.
• W2610503334 hasConcept C81885089 @default.
• W2610503334 hasConcept C86803240 @default.
• W2610503334 hasConceptScore W2610503334C104317684 @default.
• W2610503334 hasConceptScore W2610503334C121608353 @default.
• W2610503334 hasConceptScore W2610503334C126322002 @default.
• W2610503334 hasConceptScore W2610503334C142724271 @default.
• W2610503334 hasConceptScore W2610503334C143998085 @default.
• W2610503334 hasConceptScore W2610503334C150194340 @default.
• W2610503334 hasConceptScore W2610503334C2779013556 @default.
• W2610503334 hasConceptScore W2610503334C2780352672 @default.
• W2610503334 hasConceptScore W2610503334C502942594 @default.
• W2610503334 hasConceptScore W2610503334C54355233 @default.
• W2610503334 hasConceptScore W2610503334C71924100 @default.
• W2610503334 hasConceptScore W2610503334C81885089 @default.
• W2610503334 hasConceptScore W2610503334C86803240 @default.
• W2610503334 hasLocation W26105033341 @default.
• W2610503334 hasOpenAccess W2610503334 @default.
• W2610503334 hasPrimaryLocation W26105033341 @default.
• W2610503334 hasRelatedWork W1999333622 @default.
• W2610503334 hasRelatedWork W2001710827 @default.
• W2610503334 hasRelatedWork W2006300041 @default.
• W2610503334 hasRelatedWork W2013528095 @default.
• W2610503334 hasRelatedWork W2052663359 @default.
• W2610503334 hasRelatedWork W2053905288 @default.
• W2610503334 hasRelatedWork W2058073053 @default.
• W2610503334 hasRelatedWork W2070445266 @default.
• W2610503334 hasRelatedWork W2075763371 @default.
• W2610503334 hasRelatedWork W2078417558 @default.
• W2610503334 hasRelatedWork W2087638253 @default.
• W2610503334 hasRelatedWork W2118541620 @default.
• W2610503334 hasRelatedWork W2126310653 @default.
• W2610503334 hasRelatedWork W2130408744 @default.
• W2610503334 hasRelatedWork W2152411486 @default.
• W2610503334 hasRelatedWork W2170540646 @default.
• W2610503334 hasRelatedWork W2334557927 @default.
• W2610503334 hasRelatedWork W2357160441 @default.
• W2610503334 hasRelatedWork W3041050578 @default.
• W2610503334 hasRelatedWork W3047361797 @default.
• W2610503334 hasVolume "64" @default.
• W2610503334 isParatext "false" @default.
• W2610503334 isRetracted "false" @default.
• W2610503334 magId "2610503334" @default.
• W2610503334 workType "article" @default.