FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2610767504> ?p ?o ?g. }

• W2610767504 endingPage "1388" @default.
• W2610767504 startingPage "1374" @default.
• W2610767504 abstract "Summary Over the last decade, protocols aimed at reproducibly sequencing reduced‐genome subsets in non‐model organisms have been widely developed. Their use is, however, limited to DNA of relatively high molecular weight. During the last year, several methods exploiting hybridization capture using probes based on RAD ‐sequencing loci have circumvented this limitation and opened avenues to the study of samples characterized by degraded DNA , such as historical specimens. Here, we present a major update to those methods, namely hybridization capture from RAD ‐derived probes obtained from a reduced eXome template (hy RAD ‐X), a technique applying RAD sequencing to messenger RNA from one or few fresh specimens to elaborate bench‐top produced probes, that is, a reduced representation of the exome, further used to capture homologous DNA from a samples set. In contrast to previous hybridization capture methods, the reference catalogue on which reads are aligned does not rely on de novo assembly of anonymous RAD ‐sequencing loci, but on an assembled transcriptome obtained from RNA seq data, thus increasing the accuracy of loci definition and single‐nucleotide polymorphisms ( SNP ) call, and targeting, specifically, expressed genes. Finally, the capture step of hy RAD ‐X relies on RNA probes, increasing stringency of hybridization, making it well suited for low‐content DNA samples. As a proof of concept, we applied hy RAD ‐X to subfossil needles from the coniferous tree A bies alba , collected in lake sediments ( O riglio, S witzerland) and dating back from 7200 to 5800 years before present ( bp ). More specifically, we investigated genetic variation before, during and after an anthropogenic perturbation that caused an abrupt decrease in A . alba population size, 6500–6200 years bp . Hy RAD ‐X produced a matrix encompassing 524 exome‐derived SNP s. Despite a lower observed heterozygosity was found during the 6500–6200 years bp time slice, genetic composition was nearly identical before and after the perturbation, indicating that re‐expansion of the population after the decline was most likely driven by local specimens. To the best of our knowledge, this is the first time a population genomic study incorporating ancient DNA samples of tree subfossils is conducted at a moderate cost using reproducible exome‐reduced complexity." @default.
• W2610767504 created "2017-05-12" @default.
• W2610767504 creator A5004526283 @default.
• W2610767504 creator A5038118810 @default.
• W2610767504 creator A5045753481 @default.
• W2610767504 creator A5067871002 @default.
• W2610767504 creator A5073926887 @default.
• W2610767504 creator A5079435059 @default.
• W2610767504 creator A5091814951 @default.
• W2610767504 date "2017-05-07" @default.
• W2610767504 modified "2023-09-27" @default.
• W2610767504 title "Hy <scp>RAD</scp> ‐X, a versatile method combining exome capture and <scp>RAD</scp> sequencing to extract genomic information from ancient <scp>DNA</scp>" @default.
• W2610767504 cites W1525122508 @default.
• W2610767504 cites W1970406212 @default.
• W2610767504 cites W1981509058 @default.
• W2610767504 cites W1983479980 @default.
• W2610767504 cites W1996439449 @default.
• W2610767504 cites W2000948351 @default.
• W2610767504 cites W2004812123 @default.
• W2610767504 cites W2008249583 @default.
• W2610767504 cites W2013367562 @default.
• W2610767504 cites W2017344012 @default.
• W2610767504 cites W2024251716 @default.
• W2610767504 cites W2035822106 @default.
• W2610767504 cites W2037412015 @default.
• W2610767504 cites W2051778731 @default.
• W2610767504 cites W2053427298 @default.
• W2610767504 cites W2055043387 @default.
• W2610767504 cites W2069511429 @default.
• W2610767504 cites W2070720545 @default.
• W2610767504 cites W2081138730 @default.
• W2610767504 cites W2083374034 @default.
• W2610767504 cites W2091450548 @default.
• W2610767504 cites W2097065948 @default.
• W2610767504 cites W2103438734 @default.
• W2610767504 cites W2104293014 @default.
• W2610767504 cites W2106333948 @default.
• W2610767504 cites W2110065044 @default.
• W2610767504 cites W2116585889 @default.
• W2610767504 cites W2119404103 @default.
• W2610767504 cites W2120096459 @default.
• W2610767504 cites W2122374533 @default.
• W2610767504 cites W2123916548 @default.
• W2610767504 cites W2139115044 @default.
• W2610767504 cites W2140083627 @default.
• W2610767504 cites W2147634297 @default.
• W2610767504 cites W2149992227 @default.
• W2610767504 cites W2150163641 @default.
• W2610767504 cites W2159474015 @default.
• W2610767504 cites W2161184021 @default.
• W2610767504 cites W2165725065 @default.
• W2610767504 cites W2169773990 @default.
• W2610767504 cites W2170551349 @default.
• W2610767504 cites W2170725655 @default.
• W2610767504 cites W2171211989 @default.
• W2610767504 cites W2198996286 @default.
• W2610767504 cites W2470818520 @default.
• W2610767504 cites W2488889573 @default.
• W2610767504 cites W2537180905 @default.
• W2610767504 cites W2952033584 @default.
• W2610767504 cites W2952596951 @default.
• W2610767504 cites W65511526 @default.
• W2610767504 doi "https://doi.org/10.1111/2041-210x.12785" @default.
• W2610767504 hasPublicationYear "2017" @default.
• W2610767504 type Work @default.
• W2610767504 sameAs 2610767504 @default.
• W2610767504 citedByCount "44" @default.
• W2610767504 countsByYear W26107675042017 @default.
• W2610767504 countsByYear W26107675042018 @default.
• W2610767504 countsByYear W26107675042019 @default.
• W2610767504 countsByYear W26107675042020 @default.
• W2610767504 countsByYear W26107675042021 @default.
• W2610767504 countsByYear W26107675042022 @default.
• W2610767504 countsByYear W26107675042023 @default.
• W2610767504 crossrefType "journal-article" @default.
• W2610767504 hasAuthorship W2610767504A5004526283 @default.
• W2610767504 hasAuthorship W2610767504A5038118810 @default.
• W2610767504 hasAuthorship W2610767504A5045753481 @default.
• W2610767504 hasAuthorship W2610767504A5067871002 @default.
• W2610767504 hasAuthorship W2610767504A5073926887 @default.
• W2610767504 hasAuthorship W2610767504A5079435059 @default.
• W2610767504 hasAuthorship W2610767504A5091814951 @default.
• W2610767504 hasBestOaLocation W26107675041 @default.
• W2610767504 hasConcept C104317684 @default.
• W2610767504 hasConcept C153911025 @default.
• W2610767504 hasConcept C16671776 @default.
• W2610767504 hasConcept C501734568 @default.
• W2610767504 hasConcept C51679486 @default.
• W2610767504 hasConcept C54355233 @default.
• W2610767504 hasConcept C552990157 @default.
• W2610767504 hasConcept C70721500 @default.
• W2610767504 hasConcept C86803240 @default.
• W2610767504 hasConceptScore W2610767504C104317684 @default.
• W2610767504 hasConceptScore W2610767504C153911025 @default.
• W2610767504 hasConceptScore W2610767504C16671776 @default.
• W2610767504 hasConceptScore W2610767504C501734568 @default.
• W2610767504 hasConceptScore W2610767504C51679486 @default.
• W2610767504 hasConceptScore W2610767504C54355233 @default.

• next