Matches in SemOpenAlex for { <https://semopenalex.org/work/W2611247705> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2611247705 abstract "To this day, glioblastoma (GBM) remains a brain tumor impossible to cure. Among its tumor properties are rapid proliferation and aggressive migration, two hallmarks investigated in this study. GBM's rapid recurrence after treatment is attributed to tumor cells exhibiting stem cell properties, the so called brain tumor initiating cells (BTICs). These are targeted by the anti-diabetic drug metformin which has demonstrated its anti-glioma potential in previous studies. However, metformin's mechanisms and especially its links to transforming growth factor beta 2 (TGF-β2) are not yet fully understood. Therefore, this study explored the effects of different doses of metformin and a single dose of TGF-β2 on proliferation and migration of proneural and mesenchymal BTICs and their differentiated counterparts (TCs) as well as possible functional interactions. Proliferation and migration of 5 BTIC and 5 TC lines were assessed in cell counts, CyQuant assays, spheroid migration assays and scratch migration assays. The functional investigation showed that 10 mM metformin reliably reduced proliferation and migration of primary GBM cell lines and also demonstrated that low doses of metformin may inhibit proliferation of proneural BTICs. Proneural cells were more susceptible to metformin than mesenchymal cells and BTICs were more susceptible than TCs providing possible predictors for successful metformin treatment. The low-dose effects of metformin also seem attainable in brain tissue of human cancer patients. Hence, this study sets the rationale to explore higher doses of metformin in patients who may profit from metformin treatment, especially since proneural cells respond less to standard temozolomide (TMZ) treatment. The effects of TGF-β2, a cytokine held responsible for GBM's proliferation, invasion, angiogenesis and immunosuppression, were also assessed. Unexpectedly, TGF-β2 had either no effects or it decreased proliferation and migration. Generally, mesenchymal cells showed an increased sensitivity. As TGF-β2 has been described to increase proliferation and migration while metformin may lower both, this study investigated whether their functional effects were opposite. This was not the case. The effects of the combination of TGF-β2 and metformin were anti-proliferative and anti-migratory. They were either as strong as those of the single agents or stronger indicating that there is no functional opposition of the two but rather uniform effects possibly potentiating each other. Thus, this study suggests that metformin and TGF-β2 exert their functional effects independently of each other." @default.
- W2611247705 created "2017-05-12" @default.
- W2611247705 creator A5045776738 @default.
- W2611247705 date "2017-04-24" @default.
- W2611247705 modified "2023-09-26" @default.
- W2611247705 title "The Influence of Metformin and TGF-β2 on Proliferation and Migration of Glioblastoma Cells" @default.
- W2611247705 hasPublicationYear "2017" @default.
- W2611247705 type Work @default.
- W2611247705 sameAs 2611247705 @default.
- W2611247705 citedByCount "0" @default.
- W2611247705 crossrefType "dissertation" @default.
- W2611247705 hasAuthorship W2611247705A5045776738 @default.
- W2611247705 hasConcept C118131993 @default.
- W2611247705 hasConcept C134018914 @default.
- W2611247705 hasConcept C137738243 @default.
- W2611247705 hasConcept C1491633281 @default.
- W2611247705 hasConcept C173396325 @default.
- W2611247705 hasConcept C198826908 @default.
- W2611247705 hasConcept C2777389519 @default.
- W2611247705 hasConcept C2778227246 @default.
- W2611247705 hasConcept C2778229498 @default.
- W2611247705 hasConcept C2780323712 @default.
- W2611247705 hasConcept C502942594 @default.
- W2611247705 hasConcept C54355233 @default.
- W2611247705 hasConcept C555293320 @default.
- W2611247705 hasConcept C62112901 @default.
- W2611247705 hasConcept C81885089 @default.
- W2611247705 hasConcept C86803240 @default.
- W2611247705 hasConcept C95444343 @default.
- W2611247705 hasConceptScore W2611247705C118131993 @default.
- W2611247705 hasConceptScore W2611247705C134018914 @default.
- W2611247705 hasConceptScore W2611247705C137738243 @default.
- W2611247705 hasConceptScore W2611247705C1491633281 @default.
- W2611247705 hasConceptScore W2611247705C173396325 @default.
- W2611247705 hasConceptScore W2611247705C198826908 @default.
- W2611247705 hasConceptScore W2611247705C2777389519 @default.
- W2611247705 hasConceptScore W2611247705C2778227246 @default.
- W2611247705 hasConceptScore W2611247705C2778229498 @default.
- W2611247705 hasConceptScore W2611247705C2780323712 @default.
- W2611247705 hasConceptScore W2611247705C502942594 @default.
- W2611247705 hasConceptScore W2611247705C54355233 @default.
- W2611247705 hasConceptScore W2611247705C555293320 @default.
- W2611247705 hasConceptScore W2611247705C62112901 @default.
- W2611247705 hasConceptScore W2611247705C81885089 @default.
- W2611247705 hasConceptScore W2611247705C86803240 @default.
- W2611247705 hasConceptScore W2611247705C95444343 @default.
- W2611247705 hasLocation W26112477051 @default.
- W2611247705 hasOpenAccess W2611247705 @default.
- W2611247705 hasPrimaryLocation W26112477051 @default.
- W2611247705 hasRelatedWork W1508407537 @default.
- W2611247705 hasRelatedWork W1508624047 @default.
- W2611247705 hasRelatedWork W1836950607 @default.
- W2611247705 hasRelatedWork W1976993918 @default.
- W2611247705 hasRelatedWork W2014285846 @default.
- W2611247705 hasRelatedWork W2014302193 @default.
- W2611247705 hasRelatedWork W2065591959 @default.
- W2611247705 hasRelatedWork W2106365104 @default.
- W2611247705 hasRelatedWork W2188528128 @default.
- W2611247705 hasRelatedWork W2296716570 @default.
- W2611247705 hasRelatedWork W2332602247 @default.
- W2611247705 hasRelatedWork W2408195677 @default.
- W2611247705 hasRelatedWork W2411946691 @default.
- W2611247705 hasRelatedWork W2433393609 @default.
- W2611247705 hasRelatedWork W2563478049 @default.
- W2611247705 hasRelatedWork W2885851591 @default.
- W2611247705 hasRelatedWork W2895836456 @default.
- W2611247705 hasRelatedWork W2906044353 @default.
- W2611247705 hasRelatedWork W2919586790 @default.
- W2611247705 hasRelatedWork W2978249436 @default.
- W2611247705 isParatext "false" @default.
- W2611247705 isRetracted "false" @default.
- W2611247705 magId "2611247705" @default.
- W2611247705 workType "dissertation" @default.