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• W2611519180 endingPage "2084" @default.
• W2611519180 startingPage "2075" @default.
• W2611519180 abstract "ABSTRACT Utilization of renewable feedstocks for the production of bio‐based chemicals such as d ‐lactic acid by engineering metabolic pathways in the yeast Saccharomyces cerevisiae has recently become an attractive option. In this study, to realize efficient d ‐lactic acid production by S. cerevisiae , the expression of 12 glycolysis‐related genes and the Leuconostoc mesenteroides d ‐LDH gene was optimized using a previously developed global metabolic engineering strategy, and repeated batch fermentation was carried out using the resultant strain YPH499/dPdA3‐34/DLDH/1‐18. Stable d ‐lactic acid production through 10 repeated batch fermentations was achieved using YPH499/dPdA3‐34/DLDH/1‐18. The average d ‐lactic acid production, productivity, and yield with 10 repeated batch fermentations were 60.3 g/L, 2.80 g/L/h, and 0.646, respectively. The present study is the first report of the application of a global metabolic engineering strategy for bio‐based chemical production, and it shows the potential for efficient production of such chemicals by global metabolic engineering of the yeast S. cerevisiae . Biotechnol. Bioeng. 2017;114: 2075–2084. © 2017 Wiley Periodicals, Inc." @default.
• W2611519180 created "2017-05-12" @default.
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• W2611519180 date "2017-05-18" @default.
• W2611519180 modified "2023-10-17" @default.
• W2611519180 title "Enhanced <scp>d</scp> -lactic acid production by recombinant <i>Saccharomyces cerevisiae</i> following optimization of the global metabolic pathway" @default.
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• W2611519180 doi "https://doi.org/10.1002/bit.26330" @default.
• W2611519180 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28475210" @default.
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