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- W2611872068 abstract "Sepsis is a greatly feared complication of total jointarthroplasty. One key question is how to prevent periopera-tivebacterial adherence, and therefore the potential for infectiouscomplications. The objective of our study was to appraise theemerging capacity of staphylococcal survival on prostheticmaterials and to analyze the in vitro eects of gentamicin andvancomycin loaded polymethylmethacrylate (PMMA) cement onbacterial adherence and growth. Hospital acquiredstaphylococcal strains were systematically inoculated on fourorthopedic materials (ultrahigh mo-lecular weight polyethylene,PMMA without antibiotic, commercially produced PMMA loaded withgentamicin, and manually mixed PMMA loaded with gentamicin andvancomycin). Staphylococci were identied using culture andbiochemical tests. The inoculated material was allowed toincubate in a liquid broth growth media and subsequentlyprepared for scanning electron microscopy and bacterial growthquantication. Materials without antibiotics showed evidence ofstaphylococcal growth. PMMA loaded with only gentamicin grewmethicillin-resistant Staphylococcus aureus.Gentamicin-vancomycin loaded PMMA completely inhibited anybacterial growth.Low-dose gentamicin-vancomycin loaded PMMAprevents staphylococcal colonization better than commerciallymanufactured PMMA loaded with gentamicin. We recommend thiscombination in high-risk procedures and revision surgeriesrequiring bone cement." @default.
- W2611872068 created "2017-05-12" @default.
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- W2611872068 date "2005-01-01" @default.
- W2611872068 modified "2023-09-27" @default.
- W2611872068 title "In vitro testing of vancomycin - gentamicin loaded bone cementto prevent prosthetic joint infection" @default.
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