Matches in SemOpenAlex for { <https://semopenalex.org/work/W2612841749> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2612841749 abstract "Regulatory B cells, producing IL-10, have been studied in many autoimmune diseases. However, less is known about these cells in Type 1 diabetes (T1D), a disease characterized by the destruction of beta-cells by the immune system, leading to deficient production of insulin. Although B cells play a role in development of T1D, most previous investigations have focused on their pathogenic involvement. B cell depletion has been shown to be protective against diabetes development.To examine regulatory B cells in T1D, we used Non-Obese Diabetic (NOD) mice and non-diabetes-prone B6g7 mice as controls. We compared both strains for the production of cytokines and expression of putative regulatory phenotypes in spleen B cells cultured with various stimulants, at different ages. We observed that NOD mice that were > 35 weeks old and naturally protected against T1D had more IL-10-producing B cells than B6g7 and diabetic NOD mice, and this number increased even more on stimulation with lipopolysaccharide (LPS) stimulation. When LPS-stimulated B cells from protected mice were cultured in vitro with CD8 T cells and DCs, their potential for suppression of T cell cytotoxic activity was higher than unstimulated B cells and B cells from diabetic mice. This inhibitory effect was associated with higher levels of IL-10.Lastly, we carried out an investigation where B cells were transiently depleted in transgenic NOD mice expressing human CD20, to enable depletion using a human anti-CD20 monoclonal antibody. We evaluated the effect of depletion and repopulation on regulatory B cells, testing whether the protection afforded by B cell depletion was due to a change in regulatory B cell number or function. B cells with putative regulatory phenotypes were susceptible to depletion and, although the treatment with anti-CD20 reduced the incidence and delayed the onset of diabetes, there was no difference in the IL-10 producing B cell population by the time of full repopulation of B cells. Thus, this protective effect of B cell depletion was unlikely to be due to IL-10-producing B cells.In conclusion, for the first time, regulatory B cells were extensively studied in NOD mice and we demonstrated that protected NOD mice had higher frequencies of spleen B cells producing IL-10 than diabetic NOD mice. Further investigation is warranted to understand how these IL-10-producing B cells contribute to protection against type 1 diabetes." @default.
- W2612841749 created "2017-05-19" @default.
- W2612841749 creator A5001049918 @default.
- W2612841749 date "2017-01-01" @default.
- W2612841749 modified "2023-09-27" @default.
- W2612841749 title "Regulatory B cells in an experimental model of type 1 diabetes" @default.
- W2612841749 hasPublicationYear "2017" @default.
- W2612841749 type Work @default.
- W2612841749 sameAs 2612841749 @default.
- W2612841749 citedByCount "0" @default.
- W2612841749 crossrefType "dissertation" @default.
- W2612841749 hasAuthorship W2612841749A5001049918 @default.
- W2612841749 hasConcept C134018914 @default.
- W2612841749 hasConcept C154317977 @default.
- W2612841749 hasConcept C159654299 @default.
- W2612841749 hasConcept C167672396 @default.
- W2612841749 hasConcept C187316574 @default.
- W2612841749 hasConcept C202751555 @default.
- W2612841749 hasConcept C203014093 @default.
- W2612841749 hasConcept C2778013207 @default.
- W2612841749 hasConcept C2778453870 @default.
- W2612841749 hasConcept C2778754761 @default.
- W2612841749 hasConcept C2780130043 @default.
- W2612841749 hasConcept C2781438807 @default.
- W2612841749 hasConcept C55493867 @default.
- W2612841749 hasConcept C555293320 @default.
- W2612841749 hasConcept C55851684 @default.
- W2612841749 hasConcept C86803240 @default.
- W2612841749 hasConcept C8891405 @default.
- W2612841749 hasConceptScore W2612841749C134018914 @default.
- W2612841749 hasConceptScore W2612841749C154317977 @default.
- W2612841749 hasConceptScore W2612841749C159654299 @default.
- W2612841749 hasConceptScore W2612841749C167672396 @default.
- W2612841749 hasConceptScore W2612841749C187316574 @default.
- W2612841749 hasConceptScore W2612841749C202751555 @default.
- W2612841749 hasConceptScore W2612841749C203014093 @default.
- W2612841749 hasConceptScore W2612841749C2778013207 @default.
- W2612841749 hasConceptScore W2612841749C2778453870 @default.
- W2612841749 hasConceptScore W2612841749C2778754761 @default.
- W2612841749 hasConceptScore W2612841749C2780130043 @default.
- W2612841749 hasConceptScore W2612841749C2781438807 @default.
- W2612841749 hasConceptScore W2612841749C55493867 @default.
- W2612841749 hasConceptScore W2612841749C555293320 @default.
- W2612841749 hasConceptScore W2612841749C55851684 @default.
- W2612841749 hasConceptScore W2612841749C86803240 @default.
- W2612841749 hasConceptScore W2612841749C8891405 @default.
- W2612841749 hasLocation W26128417491 @default.
- W2612841749 hasOpenAccess W2612841749 @default.
- W2612841749 hasPrimaryLocation W26128417491 @default.
- W2612841749 hasRelatedWork W1483119284 @default.
- W2612841749 hasRelatedWork W1508436155 @default.
- W2612841749 hasRelatedWork W1593322963 @default.
- W2612841749 hasRelatedWork W1804122417 @default.
- W2612841749 hasRelatedWork W1995678458 @default.
- W2612841749 hasRelatedWork W2003945383 @default.
- W2612841749 hasRelatedWork W2021841160 @default.
- W2612841749 hasRelatedWork W2057764142 @default.
- W2612841749 hasRelatedWork W2059932788 @default.
- W2612841749 hasRelatedWork W2116153863 @default.
- W2612841749 hasRelatedWork W2119678854 @default.
- W2612841749 hasRelatedWork W2140886712 @default.
- W2612841749 hasRelatedWork W2156094245 @default.
- W2612841749 hasRelatedWork W2166192030 @default.
- W2612841749 hasRelatedWork W2415314409 @default.
- W2612841749 hasRelatedWork W2462776776 @default.
- W2612841749 hasRelatedWork W2736400451 @default.
- W2612841749 hasRelatedWork W2948674491 @default.
- W2612841749 hasRelatedWork W2968734108 @default.
- W2612841749 hasRelatedWork W2157706095 @default.
- W2612841749 isParatext "false" @default.
- W2612841749 isRetracted "false" @default.
- W2612841749 magId "2612841749" @default.
- W2612841749 workType "dissertation" @default.