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• W2612846952 abstract "We have developed a microfluidic system suitable to be incorporated with a metabolic imaging method to monitor the drug response of cells cultured on a chip. The cells were perfusion-cultured to mimic the blood flow in vivo. Label-free optical measurements and imaging of nicotinamide adenine dinucleotide and flavin adenine dinucleotide fluorescence intensity and morphological changes were evaluated non-invasively. Drug responses calculated using redox ratio imaging were compared with the drug toxicity testing results obtained with a traditional well-plate system. We found that our method can accurately monitor the cell viability and drug response and that the IC50 value obtained from imaging analysis was sensitive and comparable with a commonly used cell viability assay: MTS (3–(4,5-dimethylthiazol-2-yl)–5–(3-carboxymethoxyphenyl)-2–(4-sulfo-phenyl)-2H-tetrazolium) assay. Our method could serve as a fast, non-invasive, and reliable way for drug screening and toxicity testing as well as enabling real-time monitoring of in vitro cultured cells." @default.
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• W2612846952 date "2017-05-01" @default.
• W2612846952 modified "2023-09-26" @default.
• W2612846952 title "On chip two-photon metabolic imaging for drug toxicity testing" @default.
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• W2612846952 doi "https://doi.org/10.1063/1.4983615" @default.
• W2612846952 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5426952" @default.
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