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- W261294866 abstract "Survey showed that Carnation occurrence widespread of virus disease in Tuanjie coutry,Kunming city.The disease sample of Carnation showing the viral symptom was detected by EM with the negative stain method and indirect antigen-coated plate(ACP)-ELISA methods.The long linear virus particles was observed with 1000-1600 nm long and 12nm in diameter.The juice of the disease sample reacted positively to the Carnation necrotic fleck virus(CNFV)antiserum with indirect antigen-coated plate(ACP)-ELISA.The pair of primers was designed according to degenerate primer of Closterovirus group amplifying part sequence of HSP70 related gene and RT-PCR was conducted,with the total RNA respectively from positive and health leaves tissue of Carnation as template,and then the PCR production was cloned and sequenced.The positive sample of Carnation was detected by RT-PCR with pair of specific primers designed according to the already amplifying sequence.The result showed that the specific DNA band of expected size were amplified from the positive disease sample with the two pairs of primers but not from the health one.The viruse was detected from dilutions of 106 by RT-PCR with specific primers." @default.
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- W261294866 date "2010-01-01" @default.
- W261294866 modified "2023-09-23" @default.
- W261294866 title "Detection of Carnation necrotic fleck virus by RT-PCR." @default.
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