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- W2613298861 abstract "The present study had the objective to evaluate the pregnancy rates of recipient cows transferred with in vitro produced embryos according to the corpus luteum quality and embryo development stage. Girolando (n = 15) and Nelore (n = 7) embryo donors were enrolled in a OPU process. Cyclic crossed embryo recipient heifers (n = 630), BCS ≥ 3 (1 – 5 scale) were synchronized with an intravaginal device of progesterone (Sincrogest®, Ourofino, Brazil) and 2 mg of estradiol benzoate i.m. (Sincrodiol®, Ourofino, Brazil) on D0. On D5, 400 µg of cloprostenol sodium (Sincrocio®, Ourofino, Brasil) and 400 IU of eCG (Folligon®, Intervet, Brazil) were i.m. administered. On D8, intravaginal devices were removed and on D9, 1 mg of estradiol benzoate was i.m. administered, with the transfer of the embryos occurring on D16 (Girolando and Nelore in similar conditions). Recipient corpus luteum (CL) quality was evaluated by rectal palpation at the time of embryo transfer, scoring the diameter as 1, 2 or 3 (1 – small; 3 – large). Embryos were classified as initial blastocyst (Bi), blastocyst (Bl), expanded blastocyst (Bx), hatching blastocyst (Bn) and hatched blastocyst (Be). Statistical analysis considered the nonparametric binomial test of two proportions, with 5% of significance in order to determine the occurrence of differences in pregnancy rates. To determine differences of pregnancy rates among different stages of embryonic development and among the different qualities of CL, the multiple comparison test for proportions was used, with 5% significance. Pregnancy rate at 30 days after ET was similar when using Nelore (59.63%, 161/270) or Girolando (51.94%, 187/360) embryos (P>0.05). In contrast, pregnancy rate at 60 days was greater for Nelore (57.04%, P 0.05) for CL1, CL2 and CL3, as follows: CL1 = 50.9% (82/161), CL2 = 52.4% (44/84) and CL3 = 53.0% (61/115) for Girolando embryos and CL1 = 60.0% (45/75), CL2 = 56.3% (49/87) and CL3 = 62.0 (67/108) for Nelore embryos. In conclusion, pregancy results at 60 days were influenced by embryo donor breed. However, pregnancy was independent from embryo developmental stage and CL quality based on its classification by rectal palpation." @default.
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- W2613298861 date "2014-03-11" @default.
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- W2613298861 title "Pregnancy rates of embryo recipient cows synchronized with progesterone, estrogen, equine chorionic gonadotropin and prostaglandin F2α" @default.
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