FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2613305503> ?p ?o ?g. }

• W2613305503 abstract "To develop a new method to produce recombinant reprogramming proteins, cMyc, Klf4, Oct4, and Sox2, in soluble format with low cost for the generation of induced pluripotent stem cells (iPSCs).A short polypeptide sequence derived from the HIV trans-activator of transcription protein (TAT) and the nucleus localization signal (NLS) polypeptide were fused to the N terminus of the reprogramming proteins and they were constructed into pCold-SUMO vector which can extremely improve the solubility of recombinant proteins. Then these vector plasmids were transformed into E. coli BL21 (DE3) Chaperone competent cells for amplification. The solubility of these recombinant proteins was determined by SDS-PAGE and Coomassie brilliant blue staining. The recombinant proteins were purified by Ni-NTA resin and identified by Western blot. The transduction of these proteins into HEK 293T cells were evaluated by immunofluorescence staining.These four reprogramming proteins could be produced in soluble format in pCold-SUMO expression vector system with the assistance of chaperone proteins in bacteria. The proteins were purified successfully with a purity of over 70% with a relative high transduction rate into 293 cells.The results in the present study indicate the four important reprogramming proteins, cMyc, Klf4, Oct4, and Sox2, can be produced in soluble format in bacteria with low cost. Our new method thus might be expected to greatly contribute to the future study of iPSCs." @default.
• W2613305503 created "2017-05-19" @default.
• W2613305503 creator A5003903982 @default.
• W2613305503 creator A5025476789 @default.
• W2613305503 creator A5029383780 @default.
• W2613305503 creator A5030228708 @default.
• W2613305503 creator A5031606490 @default.
• W2613305503 creator A5050798631 @default.
• W2613305503 creator A5089725191 @default.
• W2613305503 date "2017-04-18" @default.
• W2613305503 modified "2023-09-23" @default.
• W2613305503 title "Soluble expression of recomb inant cMyc, Klf4, Oct4, and Sox2 proteins in bacteria and transduction into living cells" @default.
• W2613305503 cites W1913924834 @default.
• W2613305503 cites W1970781222 @default.
• W2613305503 cites W1971192878 @default.
• W2613305503 cites W1974154484 @default.
• W2613305503 cites W1985473731 @default.
• W2613305503 cites W1991037959 @default.
• W2613305503 cites W2002943691 @default.
• W2613305503 cites W2005150141 @default.
• W2613305503 cites W2008927161 @default.
• W2613305503 cites W2013160798 @default.
• W2613305503 cites W2017824216 @default.
• W2613305503 cites W2034271168 @default.
• W2613305503 cites W2046013132 @default.
• W2613305503 cites W2053911973 @default.
• W2613305503 cites W2086351605 @default.
• W2613305503 cites W2088152986 @default.
• W2613305503 cites W2095041841 @default.
• W2613305503 cites W2095122534 @default.
• W2613305503 cites W2098597036 @default.
• W2613305503 cites W2101569447 @default.
• W2613305503 cites W2104708641 @default.
• W2613305503 cites W2125987139 @default.
• W2613305503 cites W2126330617 @default.
• W2613305503 cites W2137607857 @default.
• W2613305503 cites W2138977668 @default.
• W2613305503 cites W2152483372 @default.
• W2613305503 cites W2158979552 @default.
• W2613305503 cites W2499569683 @default.
• W2613305503 doi "https://doi.org/10.18240/ijo.2017.04.10" @default.
• W2613305503 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5406633" @default.
• W2613305503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28503428" @default.
• W2613305503 hasPublicationYear "2017" @default.
• W2613305503 type Work @default.
• W2613305503 sameAs 2613305503 @default.
• W2613305503 citedByCount "2" @default.
• W2613305503 countsByYear W26133055032018 @default.
• W2613305503 countsByYear W26133055032019 @default.
• W2613305503 crossrefType "journal-article" @default.
• W2613305503 hasAuthorship W2613305503A5003903982 @default.
• W2613305503 hasAuthorship W2613305503A5025476789 @default.
• W2613305503 hasAuthorship W2613305503A5029383780 @default.
• W2613305503 hasAuthorship W2613305503A5030228708 @default.
• W2613305503 hasAuthorship W2613305503A5031606490 @default.
• W2613305503 hasAuthorship W2613305503A5050798631 @default.
• W2613305503 hasAuthorship W2613305503A5089725191 @default.
• W2613305503 hasBestOaLocation W26133055031 @default.
• W2613305503 hasConcept C104317684 @default.
• W2613305503 hasConcept C107459253 @default.
• W2613305503 hasConcept C123894998 @default.
• W2613305503 hasConcept C142613039 @default.
• W2613305503 hasConcept C145103041 @default.
• W2613305503 hasConcept C1491633281 @default.
• W2613305503 hasConcept C153911025 @default.
• W2613305503 hasConcept C156904656 @default.
• W2613305503 hasConcept C2777933648 @default.
• W2613305503 hasConcept C2778265714 @default.
• W2613305503 hasConcept C2779260011 @default.
• W2613305503 hasConcept C2780761556 @default.
• W2613305503 hasConcept C40767141 @default.
• W2613305503 hasConcept C54355233 @default.
• W2613305503 hasConcept C55493867 @default.
• W2613305503 hasConcept C74864618 @default.
• W2613305503 hasConcept C77255625 @default.
• W2613305503 hasConcept C86339819 @default.
• W2613305503 hasConcept C86803240 @default.
• W2613305503 hasConcept C95444343 @default.
• W2613305503 hasConceptScore W2613305503C104317684 @default.
• W2613305503 hasConceptScore W2613305503C107459253 @default.
• W2613305503 hasConceptScore W2613305503C123894998 @default.
• W2613305503 hasConceptScore W2613305503C142613039 @default.
• W2613305503 hasConceptScore W2613305503C145103041 @default.
• W2613305503 hasConceptScore W2613305503C1491633281 @default.
• W2613305503 hasConceptScore W2613305503C153911025 @default.
• W2613305503 hasConceptScore W2613305503C156904656 @default.
• W2613305503 hasConceptScore W2613305503C2777933648 @default.
• W2613305503 hasConceptScore W2613305503C2778265714 @default.
• W2613305503 hasConceptScore W2613305503C2779260011 @default.
• W2613305503 hasConceptScore W2613305503C2780761556 @default.
• W2613305503 hasConceptScore W2613305503C40767141 @default.
• W2613305503 hasConceptScore W2613305503C54355233 @default.
• W2613305503 hasConceptScore W2613305503C55493867 @default.
• W2613305503 hasConceptScore W2613305503C74864618 @default.
• W2613305503 hasConceptScore W2613305503C77255625 @default.
• W2613305503 hasConceptScore W2613305503C86339819 @default.
• W2613305503 hasConceptScore W2613305503C86803240 @default.
• W2613305503 hasConceptScore W2613305503C95444343 @default.
• W2613305503 hasLocation W26133055031 @default.
• W2613305503 hasLocation W26133055032 @default.

• next