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- W2613907602 abstract "Currently, the conventional methods for blood group antigens phenotyping are the standard tube and column agglutination methods. Nevertheless, both methods are expensive and require complicated and time-consuming processes. In this study, a paper-based analytical device for red cell antigens typing (RBC Ag-typing profile PADs) was developed for antigen D, K, C, E, c, e, Jk a , Jk b and P 1. The principle of the device is based on the presence or absence of hemagglutination at the PAD detection zone where the antibody has been pre-immobilized. The positive RBCs react and agglutinate with their corresponding antibodies, while the antigen negative RBCs fail to agglutinate and are eluted by phosphate buffer saline (PBS). The agglutinated or non-agglutinated RBC results can be visually read directly. The results of RBC Ag-typing profile PADs for unknown blood samples and standard cells (n = 50) were 100% correct, in comparison with the standard tube method. The RBC Ag-typing profile PADs developed in this study are fast, easy to use, economical and could be further developed for additional phenotyping of other blood group antigens. In addition, this method has a potential to be adapted to a large scale production and could be of great benefit to all blood banking laboratories." @default.
- W2613907602 created "2017-05-19" @default.
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- W2613907602 date "2016-04-01" @default.
- W2613907602 modified "2023-09-23" @default.
- W2613907602 title "Development of Red Blood Cell Antigen Phenotyping Profile Test by Paper-based Analytical Devices" @default.
- W2613907602 hasPublicationYear "2016" @default.
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