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- W2613912738 abstract "Classic Human Astrovirus (Classic HAstV) are one of the most important causes of pediatric acute gastroenteritis (AGE), after rotaviruses and arguably caliciviruses. The aim of this study was to determine the molecular epidemiology of Classic HAstV from 175 clinical samples, being 153 stools and 22 vomits, collected from pediatric hospitalized patients with AGE in Salto city, Uruguay, from January 2011 to December 2012. Classic HAstV were detected and genotyped by using a qualitative Retro Transcription—Polimerase Chain Reaction (RT‐PCR) directed to the Open Reading Frame‐2 (ORF2) region C. Amplicons were sequenced and phylogenetic analyses were carried out in order to determine genotypes and lineages. Classic HAstV were detected in 18 out of 175 analyzed samples (10.3%) and 14 of them (78.0%) were successfully sequenced being 6 (42.8%) classified as HAstV‐1 (1a lineage), 4 (28.6%) as HAstV‐2 (2c lineage), and 4 (28.6%) as HAstV‐3 (3c lineage). A higher detection of Classic HAstV infections was observed in autumn for both years of surveillance, and the majority of the positive cases were observed in 2011. The group of children between 2 and 5 years old presented the higher percentage of infections. To our knowledge, the present study represents the first report of astrovirus from acute gastroenteritis cases in Uruguay, evidencing its role as a relevant etiologic agent in severe cases of this disease." @default.
- W2613912738 created "2017-05-19" @default.
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- W2613912738 date "2017-06-06" @default.
- W2613912738 modified "2023-10-06" @default.
- W2613912738 title "Epidemiology and genetic diversity of classic human astrovirus among hospitalized children with acute gastroenteritis in Uruguay" @default.
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- W2613912738 doi "https://doi.org/10.1002/jmv.24854" @default.
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