Matches in SemOpenAlex for { <https://semopenalex.org/work/W2614493382> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2614493382 endingPage "S2" @default.
- W2614493382 startingPage "S2" @default.
- W2614493382 abstract "Introduction: The immune response after transplantation can be affected by DNA methylation – an epigenetic modification that regulates gene expression, including that of immune-related genes. Since the methylation of gene promoters generally acts to repress gene transcription, we wanted to know whether immunosuppressive drugs might control T cell function in this manner. In activated T cells, we therefore determined the effects of tacrolimus and mycophenolic acid (MPA) on DNA methylation of the gene promoter region of interferon-gamma (IFN-γ) – a pro-inflammatory cytokine gene. Methods: Naive T cells (CCR7+CD45RO-) and memory T cells (CD45RO+ and CCR7-CD45RO-) isolated from healthy donors were stimulated for 3 days with α-CD3/CD28 in the presence or absence of tacrolimus (10 ng/mL) or MPA (0.2 μg/mL). DNA methylation was quantified by pyrosequencing at two CpG sites (CpG-54 and CpG-186) in the IFN-γ promoter region. T cell differentiation and IFN-γ protein production were analyzed by flow cytometry. Results: After stimulation, naive T cells differentiated into a memory-like phenotype (CD45RO+) and this differentiation was inhibited by both immunosuppressive drugs (p=0.02). In terms of IFN γ DNA methylation, the average percentage at the two sites decreased in naive T cells from 79.3% to 69.8% (p=0.002) after stimulation. The two immunosuppressive drugs had different effects on this reduction in DNA methylation. While tacrolimus had no effect, MPA neutralized the effect of stimulation (80.7% before, and, 78.2% after stimulation). In memory T cells, DNA methylation and differentiation were unaffected by the immunosuppressive drugs. IFN-γ protein production by the memory cells on day 1 was significantly blocked by tacrolimus but not by MPA. Conclusions: In stimulated T cells, IFN-γ DNA methylation was affected by MPA but not by tacrolimus, while T cell differentiation was inhibited by both immunosuppressive drugs. These results suggest that immunosuppressive drugs induce changes in DNA methylation independently of changes in cell phenotype. Therefore, we conclude that differentiation and DNA methylation do not necessarily follow the same dynamics after immune activation of T cells." @default.
- W2614493382 created "2017-05-26" @default.
- W2614493382 creator A5006066076 @default.
- W2614493382 creator A5024222497 @default.
- W2614493382 creator A5027462713 @default.
- W2614493382 creator A5063809316 @default.
- W2614493382 creator A5091486815 @default.
- W2614493382 creator A5091596437 @default.
- W2614493382 date "2017-05-01" @default.
- W2614493382 modified "2023-10-18" @default.
- W2614493382 title "Differential Effects of Immunosuppressive Drugs on DNA Methylation in T Cells" @default.
- W2614493382 doi "https://doi.org/10.1097/01.tp.0000520293.82779.a2" @default.
- W2614493382 hasPublicationYear "2017" @default.
- W2614493382 type Work @default.
- W2614493382 sameAs 2614493382 @default.
- W2614493382 citedByCount "0" @default.
- W2614493382 crossrefType "journal-article" @default.
- W2614493382 hasAuthorship W2614493382A5006066076 @default.
- W2614493382 hasAuthorship W2614493382A5024222497 @default.
- W2614493382 hasAuthorship W2614493382A5027462713 @default.
- W2614493382 hasAuthorship W2614493382A5063809316 @default.
- W2614493382 hasAuthorship W2614493382A5091486815 @default.
- W2614493382 hasAuthorship W2614493382A5091596437 @default.
- W2614493382 hasConcept C101762097 @default.
- W2614493382 hasConcept C104317684 @default.
- W2614493382 hasConcept C148125776 @default.
- W2614493382 hasConcept C150194340 @default.
- W2614493382 hasConcept C153911025 @default.
- W2614493382 hasConcept C190727270 @default.
- W2614493382 hasConcept C203014093 @default.
- W2614493382 hasConcept C2776090121 @default.
- W2614493382 hasConcept C33288867 @default.
- W2614493382 hasConcept C41091548 @default.
- W2614493382 hasConcept C54355233 @default.
- W2614493382 hasConcept C86803240 @default.
- W2614493382 hasConcept C8891405 @default.
- W2614493382 hasConceptScore W2614493382C101762097 @default.
- W2614493382 hasConceptScore W2614493382C104317684 @default.
- W2614493382 hasConceptScore W2614493382C148125776 @default.
- W2614493382 hasConceptScore W2614493382C150194340 @default.
- W2614493382 hasConceptScore W2614493382C153911025 @default.
- W2614493382 hasConceptScore W2614493382C190727270 @default.
- W2614493382 hasConceptScore W2614493382C203014093 @default.
- W2614493382 hasConceptScore W2614493382C2776090121 @default.
- W2614493382 hasConceptScore W2614493382C33288867 @default.
- W2614493382 hasConceptScore W2614493382C41091548 @default.
- W2614493382 hasConceptScore W2614493382C54355233 @default.
- W2614493382 hasConceptScore W2614493382C86803240 @default.
- W2614493382 hasConceptScore W2614493382C8891405 @default.
- W2614493382 hasIssue "5S-3" @default.
- W2614493382 hasLocation W26144933821 @default.
- W2614493382 hasOpenAccess W2614493382 @default.
- W2614493382 hasPrimaryLocation W26144933821 @default.
- W2614493382 hasRelatedWork W2030896169 @default.
- W2614493382 hasRelatedWork W2033874367 @default.
- W2614493382 hasRelatedWork W2124167740 @default.
- W2614493382 hasRelatedWork W2769193000 @default.
- W2614493382 hasRelatedWork W2807462236 @default.
- W2614493382 hasRelatedWork W2980158117 @default.
- W2614493382 hasRelatedWork W3004424038 @default.
- W2614493382 hasRelatedWork W3032293821 @default.
- W2614493382 hasRelatedWork W4307027223 @default.
- W2614493382 hasRelatedWork W646755843 @default.
- W2614493382 hasVolume "101" @default.
- W2614493382 isParatext "false" @default.
- W2614493382 isRetracted "false" @default.
- W2614493382 magId "2614493382" @default.
- W2614493382 workType "article" @default.