Matches in SemOpenAlex for { <https://semopenalex.org/work/W2615360503> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2615360503 endingPage "e240" @default.
- W2615360503 startingPage "e231" @default.
- W2615360503 abstract "Among potential cell-based therapies, adipose-derived stem cells (ASCs) have been proposed as a promising source of stem cells for tissue regeneration. Although many recent clinical trials have investigated the use of adipose tissue or ASCs in transplantation, analysis of the microstructures of outgrowing macrosized spheroids (macrospheroids) or three-dimensional coculture of ASC spheroids and monocyte/macrophage lineages has not been performed. The aim of this study was to analyze the microstructures of murine-derived ASC macrospheroids and the growth and osteogenic potential of these macrospheroids in a three-dimensional environment and after calcification induction by coculture with monocytes.The histologic structures of murine-derived ASC macrospheroids and the expression of marker genes for multipotency within these macrospheroids were analyzed by hematoxylin and eosin staining and in situ hybridization. ASC macrospheroid microstructures were observed by transmission electron microscopy, and cell proliferation in the spheroids was analyzed. Additionally, the growth and osteogenic potential of these macrospheroids were assessed in two-dimensional and three-dimensional environments and after calcification induction by coculture with monocytes.The expression of Oct3/4, Nanog, and Sox2 was detected even in the deep zone of spheroids, although higher expression was observed at the surface. Cell proliferation was detected within the spheroid centers. Observation of spheroid microstructure revealed extracellular matrix production within the spheroid architecture. Transplantation of a spheroid on the hydroxyapatite disc resulted in three-dimensional cell growth, filling the disc. Coculture of the spheroids with monocytes led to the formation of many osteoclast-like, multinucleated cells, and calcification was observed after 3 weeks of coculture.ASC spheroids exhibited high capacity for dynamic three-dimensional growth and osteogenic differentiation. Furthermore, ASC spheroids promoted monocyte differentiation into osteoclast-like cells, which may enhance the osteogenic potential of ASC spheroids." @default.
- W2615360503 created "2017-05-26" @default.
- W2615360503 creator A5001689645 @default.
- W2615360503 creator A5017124099 @default.
- W2615360503 creator A5028753933 @default.
- W2615360503 creator A5038450850 @default.
- W2615360503 creator A5040389554 @default.
- W2615360503 creator A5071891873 @default.
- W2615360503 creator A5084436905 @default.
- W2615360503 date "2017-07-01" @default.
- W2615360503 modified "2023-09-27" @default.
- W2615360503 title "Osteogenic Potential of Adipose-Derived Macrospheroids Cocultured with CD11b+ Monocytes" @default.
- W2615360503 doi "https://doi.org/10.11607/jomi.4957" @default.
- W2615360503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28518185" @default.
- W2615360503 hasPublicationYear "2017" @default.
- W2615360503 type Work @default.
- W2615360503 sameAs 2615360503 @default.
- W2615360503 citedByCount "1" @default.
- W2615360503 countsByYear W26153605032021 @default.
- W2615360503 crossrefType "journal-article" @default.
- W2615360503 hasAuthorship W2615360503A5001689645 @default.
- W2615360503 hasAuthorship W2615360503A5017124099 @default.
- W2615360503 hasAuthorship W2615360503A5028753933 @default.
- W2615360503 hasAuthorship W2615360503A5038450850 @default.
- W2615360503 hasAuthorship W2615360503A5040389554 @default.
- W2615360503 hasAuthorship W2615360503A5071891873 @default.
- W2615360503 hasAuthorship W2615360503A5084436905 @default.
- W2615360503 hasConcept C104317684 @default.
- W2615360503 hasConcept C107459253 @default.
- W2615360503 hasConcept C126322002 @default.
- W2615360503 hasConcept C142724271 @default.
- W2615360503 hasConcept C145103041 @default.
- W2615360503 hasConcept C171089720 @default.
- W2615360503 hasConcept C175369904 @default.
- W2615360503 hasConcept C185592680 @default.
- W2615360503 hasConcept C189165786 @default.
- W2615360503 hasConcept C202751555 @default.
- W2615360503 hasConcept C207701140 @default.
- W2615360503 hasConcept C2777933648 @default.
- W2615360503 hasConcept C28328180 @default.
- W2615360503 hasConcept C2911091166 @default.
- W2615360503 hasConcept C55493867 @default.
- W2615360503 hasConcept C71924100 @default.
- W2615360503 hasConcept C86803240 @default.
- W2615360503 hasConcept C95444343 @default.
- W2615360503 hasConceptScore W2615360503C104317684 @default.
- W2615360503 hasConceptScore W2615360503C107459253 @default.
- W2615360503 hasConceptScore W2615360503C126322002 @default.
- W2615360503 hasConceptScore W2615360503C142724271 @default.
- W2615360503 hasConceptScore W2615360503C145103041 @default.
- W2615360503 hasConceptScore W2615360503C171089720 @default.
- W2615360503 hasConceptScore W2615360503C175369904 @default.
- W2615360503 hasConceptScore W2615360503C185592680 @default.
- W2615360503 hasConceptScore W2615360503C189165786 @default.
- W2615360503 hasConceptScore W2615360503C202751555 @default.
- W2615360503 hasConceptScore W2615360503C207701140 @default.
- W2615360503 hasConceptScore W2615360503C2777933648 @default.
- W2615360503 hasConceptScore W2615360503C28328180 @default.
- W2615360503 hasConceptScore W2615360503C2911091166 @default.
- W2615360503 hasConceptScore W2615360503C55493867 @default.
- W2615360503 hasConceptScore W2615360503C71924100 @default.
- W2615360503 hasConceptScore W2615360503C86803240 @default.
- W2615360503 hasConceptScore W2615360503C95444343 @default.
- W2615360503 hasIssue "4" @default.
- W2615360503 hasLocation W26153605031 @default.
- W2615360503 hasLocation W26153605032 @default.
- W2615360503 hasOpenAccess W2615360503 @default.
- W2615360503 hasPrimaryLocation W26153605031 @default.
- W2615360503 hasRelatedWork W1927705775 @default.
- W2615360503 hasRelatedWork W1978560791 @default.
- W2615360503 hasRelatedWork W2009589877 @default.
- W2615360503 hasRelatedWork W2148445634 @default.
- W2615360503 hasRelatedWork W2623169293 @default.
- W2615360503 hasRelatedWork W2943284045 @default.
- W2615360503 hasRelatedWork W4200352330 @default.
- W2615360503 hasRelatedWork W4224094228 @default.
- W2615360503 hasRelatedWork W4381158280 @default.
- W2615360503 hasRelatedWork W752603115 @default.
- W2615360503 hasVolume "32" @default.
- W2615360503 isParatext "false" @default.
- W2615360503 isRetracted "false" @default.
- W2615360503 magId "2615360503" @default.
- W2615360503 workType "article" @default.