FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2615391284> ?p ?o ?g. }

• W2615391284 endingPage "362" @default.
• W2615391284 startingPage "355" @default.
• W2615391284 abstract "Biogenic amines are indispensable components of living cells; nevertheless these compounds could be toxic for human health in higher concentrations. Putrescine is supposed to be the major biogenic amine associated with microbial food spoilage. Development of reliable, fast and culture-independent molecular methods to detect bacteria producing biogenic amines deserves the attention, especially of the food industry in purpose to protect health. The objective of this study was to verify the newly designed primer sets for detection of two inducible genes adiA and speF together in Salmonella enterica and Escherichia coli genome by Real-time PCR. These forenamed genes encode enzymes in the metabolic pathway which leads to production of putrescine in Gram-negative bacteria. Moreover, relative expression of these genes was studied in E. coli CCM 3954 strain using Real-time PCR. In this study, sets of new primers for the detection two inducible genes (speF and adiA) in Salmonella enterica and E. coli by Real-time PCR were designed and tested. Amplification efficiency of a Real-time PCR was calculated from the slope of the standard curves (adiA, speF, gapA). An efficiency in a range from 95 to 105 % for all tested reactions was achieved. The gene expression (R) of adiA and speF genes in E. coli was varied depending on culture conditions. The highest gene expression of adiA and speF was observed at 6, 24 and 36 h (RadiA ~ 3, 5, 9; RspeF ~11, 10, 9; respectively) after initiation of growth of this bacteria in nutrient broth medium enchired with amino acids. The results show that these primers could be used for relative quantification analysis of E. coli." @default.
• W2615391284 created "2017-05-26" @default.
• W2615391284 creator A5012375686 @default.
• W2615391284 creator A5028804472 @default.
• W2615391284 creator A5033325888 @default.
• W2615391284 creator A5034380749 @default.
• W2615391284 creator A5052330826 @default.
• W2615391284 date "2017-05-17" @default.
• W2615391284 modified "2023-09-26" @default.
• W2615391284 title "Quantitative Real-time PCR detection of putrescine-producing Gram-negative bacteria" @default.
• W2615391284 cites W1534895249 @default.
• W2615391284 cites W1607256045 @default.
• W2615391284 cites W1901827032 @default.
• W2615391284 cites W1976180036 @default.
• W2615391284 cites W1983514727 @default.
• W2615391284 cites W1995666413 @default.
• W2615391284 cites W2009608644 @default.
• W2615391284 cites W2010978022 @default.
• W2615391284 cites W2013759663 @default.
• W2615391284 cites W2022042918 @default.
• W2615391284 cites W2032812222 @default.
• W2615391284 cites W2046653515 @default.
• W2615391284 cites W2053858933 @default.
• W2615391284 cites W2059689436 @default.
• W2615391284 cites W2063825440 @default.
• W2615391284 cites W2069409789 @default.
• W2615391284 cites W2074800418 @default.
• W2615391284 cites W2077505423 @default.
• W2615391284 cites W2078520938 @default.
• W2615391284 cites W2078801284 @default.
• W2615391284 cites W2108244474 @default.
• W2615391284 cites W2126449709 @default.
• W2615391284 cites W2128367977 @default.
• W2615391284 cites W2129742279 @default.
• W2615391284 cites W2156675424 @default.
• W2615391284 cites W2163216014 @default.
• W2615391284 cites W2168085883 @default.
• W2615391284 cites W4232074999 @default.
• W2615391284 cites W85380906 @default.
• W2615391284 doi "https://doi.org/10.5219/739" @default.
• W2615391284 hasPublicationYear "2017" @default.
• W2615391284 type Work @default.
• W2615391284 sameAs 2615391284 @default.
• W2615391284 citedByCount "2" @default.
• W2615391284 countsByYear W26153912842020 @default.
• W2615391284 countsByYear W26153912842021 @default.
• W2615391284 crossrefType "journal-article" @default.
• W2615391284 hasAuthorship W2615391284A5012375686 @default.
• W2615391284 hasAuthorship W2615391284A5028804472 @default.
• W2615391284 hasAuthorship W2615391284A5033325888 @default.
• W2615391284 hasAuthorship W2615391284A5034380749 @default.
• W2615391284 hasAuthorship W2615391284A5052330826 @default.
• W2615391284 hasBestOaLocation W26153912841 @default.
• W2615391284 hasConcept C104317684 @default.
• W2615391284 hasConcept C178790620 @default.
• W2615391284 hasConcept C181199279 @default.
• W2615391284 hasConcept C185592680 @default.
• W2615391284 hasConcept C2777167505 @default.
• W2615391284 hasConcept C2777563447 @default.
• W2615391284 hasConcept C2779607309 @default.
• W2615391284 hasConcept C2781065037 @default.
• W2615391284 hasConcept C31903555 @default.
• W2615391284 hasConcept C48023723 @default.
• W2615391284 hasConcept C49105822 @default.
• W2615391284 hasConcept C523546767 @default.
• W2615391284 hasConcept C54355233 @default.
• W2615391284 hasConcept C547475151 @default.
• W2615391284 hasConcept C55493867 @default.
• W2615391284 hasConcept C86803240 @default.
• W2615391284 hasConcept C89423630 @default.
• W2615391284 hasConceptScore W2615391284C104317684 @default.
• W2615391284 hasConceptScore W2615391284C178790620 @default.
• W2615391284 hasConceptScore W2615391284C181199279 @default.
• W2615391284 hasConceptScore W2615391284C185592680 @default.
• W2615391284 hasConceptScore W2615391284C2777167505 @default.
• W2615391284 hasConceptScore W2615391284C2777563447 @default.
• W2615391284 hasConceptScore W2615391284C2779607309 @default.
• W2615391284 hasConceptScore W2615391284C2781065037 @default.
• W2615391284 hasConceptScore W2615391284C31903555 @default.
• W2615391284 hasConceptScore W2615391284C48023723 @default.
• W2615391284 hasConceptScore W2615391284C49105822 @default.
• W2615391284 hasConceptScore W2615391284C523546767 @default.
• W2615391284 hasConceptScore W2615391284C54355233 @default.
• W2615391284 hasConceptScore W2615391284C547475151 @default.
• W2615391284 hasConceptScore W2615391284C55493867 @default.
• W2615391284 hasConceptScore W2615391284C86803240 @default.
• W2615391284 hasConceptScore W2615391284C89423630 @default.
• W2615391284 hasIssue "1" @default.
• W2615391284 hasLocation W26153912841 @default.
• W2615391284 hasLocation W26153912842 @default.
• W2615391284 hasOpenAccess W2615391284 @default.
• W2615391284 hasPrimaryLocation W26153912841 @default.
• W2615391284 hasRelatedWork W1501404116 @default.
• W2615391284 hasRelatedWork W1969622237 @default.
• W2615391284 hasRelatedWork W1986294255 @default.
• W2615391284 hasRelatedWork W2021938393 @default.
• W2615391284 hasRelatedWork W2033112469 @default.
• W2615391284 hasRelatedWork W2034006825 @default.

• next