FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2615566422> ?p ?o ?g. }

• W2615566422 endingPage "H163" @default.
• W2615566422 startingPage "H149" @default.
• W2615566422 abstract "G protein-coupled receptor kinase isoform 2 (GRK2) has a critical role in physiological and pharmacological responses to endogenous and exogenous substances. Sepsis causes an important cardiovascular dysfunction in which nitric oxide (NO) has a relevant role. The present study aimed to assess the putative effect of inducible NO synthase (NOS2)-derived NO on the activity of GRK2 in the context of septic cardiac dysfunction. C57BL/6 mice were submitted to severe septic injury by cecal ligation and puncture (CLP). Heart function was assessed by isolated and perfused heart, echocardiography, and β-adrenergic receptor binding. GRK2 was determined by immunofluorescence and Western blot analysis in the heart and isolated cardiac myocytes. Sepsis increased NOS2 expression in the heart, increased plasma nitrite + nitrate levels, and reduced isoproterenol-induced isolated ventricle contraction, whole heart tension development, and β-adrenergic receptor density. Treatment with 1400W or with GRK2 inhibitor prevented CLP-induced cardiac hyporesponsiveness 12 and 24 h after CLP. Increased labeling of total and phosphorylated GRK2 was detected in hearts after CLP. With treatment of 1400W or in hearts taken from septic NOS2 knockout mice, the activation of GRK2 was reduced. 1400W or GRK2 inhibitor reduced mortality, improved echocardiographic cardiac parameters, and prevented organ damage. Therefore, during sepsis, NOS2-derived NO increases GRK2, which leads to a reduction in β-adrenergic receptor density, contributing to the heart dysfunction. Isolated cardiac myocyte data indicate that NO acts through the soluble guanylyl cyclase/cGMP/PKG pathway. GRK2 inhibition may be a potential therapeutic target in sepsis-induced cardiac dysfunction.NEW & NOTEWORTHY The main novelty presented here is to show that septic shock induces cardiac hyporesponsiveness to isoproterenol by a mechanism dependent on nitric oxide and mediated by G protein-coupled receptor kinase isoform 2. Therefore, G protein-coupled receptor kinase isoform 2 inhibition may be a potential therapeutic target in sepsis-induced cardiac dysfunction." @default.
• W2615566422 created "2017-05-26" @default.
• W2615566422 creator A5000312826 @default.
• W2615566422 creator A5004054624 @default.
• W2615566422 creator A5005886154 @default.
• W2615566422 creator A5024129764 @default.
• W2615566422 creator A5026728941 @default.
• W2615566422 creator A5030831755 @default.
• W2615566422 creator A5031110727 @default.
• W2615566422 creator A5036965760 @default.
• W2615566422 creator A5046750521 @default.
• W2615566422 creator A5050684767 @default.
• W2615566422 creator A5058084032 @default.
• W2615566422 creator A5063552393 @default.
• W2615566422 creator A5066521083 @default.
• W2615566422 creator A5068185709 @default.
• W2615566422 creator A5072373207 @default.
• W2615566422 creator A5080251507 @default.
• W2615566422 date "2017-07-01" @default.
• W2615566422 modified "2023-10-03" @default.
• W2615566422 title "Cardiac hyporesponsiveness in severe sepsis is associated with nitric oxide-dependent activation of G protein receptor kinase" @default.
• W2615566422 cites W1851692042 @default.
• W2615566422 cites W1915572763 @default.
• W2615566422 cites W1964171066 @default.
• W2615566422 cites W1969795252 @default.
• W2615566422 cites W1971900596 @default.
• W2615566422 cites W1973273589 @default.
• W2615566422 cites W1977190827 @default.
• W2615566422 cites W1977905350 @default.
• W2615566422 cites W1984332977 @default.
• W2615566422 cites W1984793344 @default.
• W2615566422 cites W1989707466 @default.
• W2615566422 cites W1992594024 @default.
• W2615566422 cites W1997899268 @default.
• W2615566422 cites W2005053677 @default.
• W2615566422 cites W2006713897 @default.
• W2615566422 cites W2013511908 @default.
• W2615566422 cites W2023945291 @default.
• W2615566422 cites W2026927834 @default.
• W2615566422 cites W2039104446 @default.
• W2615566422 cites W2045010042 @default.
• W2615566422 cites W2052391094 @default.
• W2615566422 cites W2053060395 @default.
• W2615566422 cites W2053800813 @default.
• W2615566422 cites W2055166414 @default.
• W2615566422 cites W2059919221 @default.
• W2615566422 cites W2063129749 @default.
• W2615566422 cites W2070715087 @default.
• W2615566422 cites W2072950045 @default.
• W2615566422 cites W2077881877 @default.
• W2615566422 cites W2085351880 @default.
• W2615566422 cites W2087084849 @default.
• W2615566422 cites W2087246147 @default.
• W2615566422 cites W2094754558 @default.
• W2615566422 cites W2101950940 @default.
• W2615566422 cites W2115929723 @default.
• W2615566422 cites W2118579624 @default.
• W2615566422 cites W2121896233 @default.
• W2615566422 cites W2122386753 @default.
• W2615566422 cites W2135289666 @default.
• W2615566422 cites W2146578470 @default.
• W2615566422 cites W2149592972 @default.
• W2615566422 cites W2161089523 @default.
• W2615566422 cites W2166414244 @default.
• W2615566422 cites W2171561094 @default.
• W2615566422 cites W2315132931 @default.
• W2615566422 cites W2335088247 @default.
• W2615566422 cites W2339610197 @default.
• W2615566422 cites W2444648729 @default.
• W2615566422 cites W4210712363 @default.
• W2615566422 cites W4293247451 @default.
• W2615566422 doi "https://doi.org/10.1152/ajpheart.00052.2016" @default.
• W2615566422 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28526706" @default.
• W2615566422 hasPublicationYear "2017" @default.
• W2615566422 type Work @default.
• W2615566422 sameAs 2615566422 @default.
• W2615566422 citedByCount "22" @default.
• W2615566422 countsByYear W26155664222018 @default.
• W2615566422 countsByYear W26155664222019 @default.
• W2615566422 countsByYear W26155664222020 @default.
• W2615566422 countsByYear W26155664222021 @default.
• W2615566422 countsByYear W26155664222022 @default.
• W2615566422 countsByYear W26155664222023 @default.
• W2615566422 crossrefType "journal-article" @default.
• W2615566422 hasAuthorship W2615566422A5000312826 @default.
• W2615566422 hasAuthorship W2615566422A5004054624 @default.
• W2615566422 hasAuthorship W2615566422A5005886154 @default.
• W2615566422 hasAuthorship W2615566422A5024129764 @default.
• W2615566422 hasAuthorship W2615566422A5026728941 @default.
• W2615566422 hasAuthorship W2615566422A5030831755 @default.
• W2615566422 hasAuthorship W2615566422A5031110727 @default.
• W2615566422 hasAuthorship W2615566422A5036965760 @default.
• W2615566422 hasAuthorship W2615566422A5046750521 @default.
• W2615566422 hasAuthorship W2615566422A5050684767 @default.
• W2615566422 hasAuthorship W2615566422A5058084032 @default.
• W2615566422 hasAuthorship W2615566422A5063552393 @default.
• W2615566422 hasAuthorship W2615566422A5066521083 @default.
• W2615566422 hasAuthorship W2615566422A5068185709 @default.

• next