Matches in SemOpenAlex for { <https://semopenalex.org/work/W2615681311> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2615681311 abstract "Lentiviral transduction followed by puromycin selection is a well-recognized procedure for gene transfer and expression experiments using a variety of cell types including human hematopoietic stem and progenitor cells. Despite its widespread application, research regarding the potential effects of bacterial puromycin N-acetyltransferase (pac) gene expression in mammalian cell cultures is incomplete. Here the potential for puromycin selection to affect transcriptome profiles was examined using a well-studied model for human erythropoiesis. Experiments were performed using primary CD34(+) cells from six adult healthy human donors transduced with two commercially available pac-encoding lentiviral vectors and compared to non-transduced control cells. RNA-Seq gene expression profiles were generated at the proerythroblast stage of differentiation, then differential gene expression was analyzed with DEseq2 in R-Studio software. Inter-donor variation in the gene expression profiles and variations between puromycin selected populations after transduction of the separate lentiviral vectors was manifested by significant differences in the RNA detection levels of less than 0.1%. However, puromycin selection after pac gene transduction caused significant changes in over 5% of the mRNA when compared to non-transduced controls. The results suggest that consideration should be given for the potential of puromycin selection to confound the interpretation of RNA-Seq transcriptome profiles." @default.
- W2615681311 created "2017-05-26" @default.
- W2615681311 creator A5010397104 @default.
- W2615681311 creator A5021421232 @default.
- W2615681311 creator A5042291217 @default.
- W2615681311 creator A5063358232 @default.
- W2615681311 date "2017-01-01" @default.
- W2615681311 modified "2023-09-26" @default.
- W2615681311 title "Puromycin Selection Confounds the RNA-Seq Profiles of Primary Human Erythroblasts" @default.
- W2615681311 doi "https://doi.org/10.4172/2329-8936.1000140" @default.
- W2615681311 hasPublicationYear "2017" @default.
- W2615681311 type Work @default.
- W2615681311 sameAs 2615681311 @default.
- W2615681311 citedByCount "3" @default.
- W2615681311 countsByYear W26156813112018 @default.
- W2615681311 countsByYear W26156813112019 @default.
- W2615681311 countsByYear W26156813112020 @default.
- W2615681311 crossrefType "journal-article" @default.
- W2615681311 hasAuthorship W2615681311A5010397104 @default.
- W2615681311 hasAuthorship W2615681311A5021421232 @default.
- W2615681311 hasAuthorship W2615681311A5042291217 @default.
- W2615681311 hasAuthorship W2615681311A5063358232 @default.
- W2615681311 hasBestOaLocation W26156813111 @default.
- W2615681311 hasConcept C104317684 @default.
- W2615681311 hasConcept C150194340 @default.
- W2615681311 hasConcept C15152581 @default.
- W2615681311 hasConcept C153911025 @default.
- W2615681311 hasConcept C162317418 @default.
- W2615681311 hasConcept C2779200571 @default.
- W2615681311 hasConcept C3675279 @default.
- W2615681311 hasConcept C54355233 @default.
- W2615681311 hasConcept C55493867 @default.
- W2615681311 hasConcept C86803240 @default.
- W2615681311 hasConcept C95444343 @default.
- W2615681311 hasConceptScore W2615681311C104317684 @default.
- W2615681311 hasConceptScore W2615681311C150194340 @default.
- W2615681311 hasConceptScore W2615681311C15152581 @default.
- W2615681311 hasConceptScore W2615681311C153911025 @default.
- W2615681311 hasConceptScore W2615681311C162317418 @default.
- W2615681311 hasConceptScore W2615681311C2779200571 @default.
- W2615681311 hasConceptScore W2615681311C3675279 @default.
- W2615681311 hasConceptScore W2615681311C54355233 @default.
- W2615681311 hasConceptScore W2615681311C55493867 @default.
- W2615681311 hasConceptScore W2615681311C86803240 @default.
- W2615681311 hasConceptScore W2615681311C95444343 @default.
- W2615681311 hasIssue "01" @default.
- W2615681311 hasLocation W26156813111 @default.
- W2615681311 hasOpenAccess W2615681311 @default.
- W2615681311 hasPrimaryLocation W26156813111 @default.
- W2615681311 hasRelatedWork W1466315383 @default.
- W2615681311 hasRelatedWork W1763607657 @default.
- W2615681311 hasRelatedWork W1977957409 @default.
- W2615681311 hasRelatedWork W2001406627 @default.
- W2615681311 hasRelatedWork W2004869583 @default.
- W2615681311 hasRelatedWork W2004898616 @default.
- W2615681311 hasRelatedWork W2098809361 @default.
- W2615681311 hasRelatedWork W2129059405 @default.
- W2615681311 hasRelatedWork W2159091439 @default.
- W2615681311 hasRelatedWork W2528001541 @default.
- W2615681311 hasRelatedWork W2549063601 @default.
- W2615681311 hasRelatedWork W2559686570 @default.
- W2615681311 hasRelatedWork W2562046372 @default.
- W2615681311 hasRelatedWork W2775437905 @default.
- W2615681311 hasRelatedWork W2791194189 @default.
- W2615681311 hasRelatedWork W2898202447 @default.
- W2615681311 hasRelatedWork W2949618019 @default.
- W2615681311 hasRelatedWork W2952887658 @default.
- W2615681311 hasRelatedWork W3120770638 @default.
- W2615681311 hasRelatedWork W3193206461 @default.
- W2615681311 hasVolume "05" @default.
- W2615681311 isParatext "false" @default.
- W2615681311 isRetracted "false" @default.
- W2615681311 magId "2615681311" @default.
- W2615681311 workType "article" @default.