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• W261617534 abstract "Research Article1 April 1992free access The ras1 function of Schizosaccharomyces pombe mediates pheromone-induced transcription. O. Nielsen O. Nielsen Institute of Genetics, University of Copenhagen, Denmark. Search for more papers by this author J. Davey J. Davey Institute of Genetics, University of Copenhagen, Denmark. Search for more papers by this author R. Egel R. Egel Institute of Genetics, University of Copenhagen, Denmark. Search for more papers by this author O. Nielsen O. Nielsen Institute of Genetics, University of Copenhagen, Denmark. Search for more papers by this author J. Davey J. Davey Institute of Genetics, University of Copenhagen, Denmark. Search for more papers by this author R. Egel R. Egel Institute of Genetics, University of Copenhagen, Denmark. Search for more papers by this author Author Information O. Nielsen1, J. Davey1 and R. Egel1 1Institute of Genetics, University of Copenhagen, Denmark. The EMBO Journal (1992)11:1391-1395https://doi.org/10.1002/j.1460-2075.1992.tb05184.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Loss of ras1+ function renders fission yeast cells unable to undergo morphological changes in response to mating pheromones, whereas cells carrying activated mutations in ras1 are hyper-responsive. This has led to the suggestion that the ras1 gene product plays a role in mating pheromone signal transduction. Using partially purified M factor we demonstrate that the mat1-Pm gene, which controls entry into meiosis, is transcribed in response to a pheromone signal. Strains mutated in the ras1 gene or in ste6, the fission yeast homologue of Ras protein GDP/GTP exchange factor, are unable to induce transcription of mat1-Pm in response to M factor. Furthermore, an activated ras1val17 mutant exhibits a stronger induction of the mat1-Pm transcript. However, transcription still depends on nitrogen deprivation as well as on the presence of pheromone, showing that activation of the Ras1 protein alone does not substitute for any of these signals. The pat1-114 mutant bypasses the ras1/ste6 checkpoint, suggesting that activation of ras1 contributes to inactivation of the pat1 protein kinase. Previous ArticleNext Article Volume 11Issue 41 April 1992In this issue RelatedDetailsLoading ..." @default.
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• W261617534 title "The ras1 function of Schizosaccharomyces pombe mediates pheromone-induced transcription." @default.
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• W261617534 doi "https://doi.org/10.1002/j.1460-2075.1992.tb05184.x" @default.
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