FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W261628470> ?p ?o ?g. }

• W261628470 endingPage "620" @default.
• W261628470 startingPage "620" @default.
• W261628470 abstract "The effectiveness of the symbiosis established between rhizobia and legumes results of a variety of biotic and abiotic conditions. For example, symbiotic gene was correlated with symbiotic effectiveness in Bradyrhizobium (Urtz and Elkan, 1996). It has also been reported that genes involved in physiology are important for an effective symbiosis. Batista et al. (1992) reported mutants of Sinorhizobium meliloti form ineffective nodules. In this study, RFLP analysis was used to examine the correlation between loci polymorphism and effective symbiosis. Five gene probes were chosen to detect changes in the genes responsible for : the synthesis of nitrogenase (nifH and nifA); nodule development (nodA); transport of (dctA) and the degradation of rhizopine (mocA), which was hypothesized confers a competitive advantage upon the bacterial strains harboring it (Rossbach et al. 1995). Total DNA’s of 24 rhizobia (19 S. meliloti, 3 R. type Oregon strains and one R. tropici) were isolated and digested with EcoRI and BamHl. DNA probes were synthesized from Sm 2011 by PCR (Haukka et al. 1998) and labeled using biotinylated random primers. Southern blot, hybridization and chemiluminescent detection were carried out using standard protocols. Temperatures for hybridization procedure were : 55°C for nif, dct, and moc probes and 68°C for nod probe. Symbiotic effectiveness was determined by measuring shoot dry-matter of alfalfa plants grown in Jensen-agar medium. We did not reveal any hybridization pattern with digested DNA from R. type Oregon strain CE20. OR191 and CE26 strains hybridized with nodA and ni fH probes respectively. Any R. type Oregon strains hybridized with the regulatory gene nifA of Sm. These results could be expected since these strains induce ineffective nodules in alfalfa. All Sm isolates, except one, had identical or nearly identical RFLP pattern when nif H gene probe was used. However, when nifA probe was used, 5 different pattern (DNA restricted with EcoRI) were observed on 19 strains, suggesting notably diversity among nif A gene. No polymorphism among nodA gene of Sm isolates were revealed on DNA digested with both restriction enzymes. The group of 23 strains were analyzed by RFLP employing a PCR fragment containing part of Sm L5-30 moc A as probe. Only seven strains revealed an hybridization pattern, suggesting that the gene responsible to catabolize rhizopine are not extensively present in this rhizobia population. Results obtained by competitiveness studies indicated that some of those seven strains referred above, were more competitive. A possible correlation between the presence of a RFLP pattern with moc A and the ability to compete should be confirmed. All the restricted DNAs hybridized with dctA probe. The RFLP pattern obtained from Sm strains showed no diversity between them. However, R. type Oregon strains revealed a distinctive pattern compared with Sm isolates. The future of this work will be focused in the establishment of a more precise correlation between the presence of molecular markers obtained with mocA gene and the competitive ability of Sinorhizobium strains." @default.
• W261628470 created "2016-06-24" @default.
• W261628470 creator A5002453222 @default.
• W261628470 creator A5007597786 @default.
• W261628470 creator A5030154889 @default.
• W261628470 creator A5048641010 @default.
• W261628470 creator A5063135402 @default.
• W261628470 creator A5068395292 @default.
• W261628470 date "2005-12-27" @default.
• W261628470 modified "2023-09-26" @default.
• W261628470 title "Diversity of NOD, NIF, DCT, and MOC Genes and Their Relation with an Effective Symbiosis" @default.
• W261628470 cites W1923996615 @default.
• W261628470 cites W1967133603 @default.
• W261628470 cites W1992325759 @default.
• W261628470 cites W2335750816 @default.
• W261628470 doi "https://doi.org/10.1007/0-306-47615-0_360" @default.
• W261628470 hasPublicationYear "2005" @default.
• W261628470 type Work @default.
• W261628470 sameAs 261628470 @default.
• W261628470 citedByCount "0" @default.
• W261628470 crossrefType "book-chapter" @default.
• W261628470 hasAuthorship W261628470A5002453222 @default.
• W261628470 hasAuthorship W261628470A5007597786 @default.
• W261628470 hasAuthorship W261628470A5030154889 @default.
• W261628470 hasAuthorship W261628470A5048641010 @default.
• W261628470 hasAuthorship W261628470A5063135402 @default.
• W261628470 hasAuthorship W261628470A5068395292 @default.
• W261628470 hasConcept C104317684 @default.
• W261628470 hasConcept C130197536 @default.
• W261628470 hasConcept C133307689 @default.
• W261628470 hasConcept C181440489 @default.
• W261628470 hasConcept C2776815337 @default.
• W261628470 hasConcept C2777304268 @default.
• W261628470 hasConcept C2777465193 @default.
• W261628470 hasConcept C2779856404 @default.
• W261628470 hasConcept C2781385570 @default.
• W261628470 hasConcept C43144210 @default.
• W261628470 hasConcept C523546767 @default.
• W261628470 hasConcept C54355233 @default.
• W261628470 hasConcept C86803240 @default.
• W261628470 hasConcept C89423630 @default.
• W261628470 hasConceptScore W261628470C104317684 @default.
• W261628470 hasConceptScore W261628470C130197536 @default.
• W261628470 hasConceptScore W261628470C133307689 @default.
• W261628470 hasConceptScore W261628470C181440489 @default.
• W261628470 hasConceptScore W261628470C2776815337 @default.
• W261628470 hasConceptScore W261628470C2777304268 @default.
• W261628470 hasConceptScore W261628470C2777465193 @default.
• W261628470 hasConceptScore W261628470C2779856404 @default.
• W261628470 hasConceptScore W261628470C2781385570 @default.
• W261628470 hasConceptScore W261628470C43144210 @default.
• W261628470 hasConceptScore W261628470C523546767 @default.
• W261628470 hasConceptScore W261628470C54355233 @default.
• W261628470 hasConceptScore W261628470C86803240 @default.
• W261628470 hasConceptScore W261628470C89423630 @default.
• W261628470 hasLocation W2616284701 @default.
• W261628470 hasOpenAccess W261628470 @default.
• W261628470 hasPrimaryLocation W2616284701 @default.
• W261628470 hasRelatedWork W2038209199 @default.
• W261628470 hasRelatedWork W2060958215 @default.
• W261628470 hasRelatedWork W2117624192 @default.
• W261628470 hasRelatedWork W2156674132 @default.
• W261628470 hasRelatedWork W2162198338 @default.
• W261628470 hasRelatedWork W2198862572 @default.
• W261628470 hasRelatedWork W2339944416 @default.
• W261628470 hasRelatedWork W2785608009 @default.
• W261628470 hasRelatedWork W4245000533 @default.
• W261628470 hasRelatedWork W4285043413 @default.
• W261628470 isParatext "false" @default.
• W261628470 isRetracted "false" @default.
• W261628470 magId "261628470" @default.
• W261628470 workType "book-chapter" @default.