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- W2616302590 abstract "SOX2 is an important transcription factor involved in pluripotency maintenance, pluripotent reprogramming and differentiation towards neural lineages. Here we engineered the previously described HEL24.3 hiPSC to generate a SOX2 reporter by knocking-in a T2A fused nuclear tdTomato reporter cassette before the STOP codon of the SOX2 gene coding sequence. CRISPR/SaCas9-mediated stimulation of homologous recombination was utilized to facilitate faithful targeted insertion. This line accurately reports the expression of endogenous SOX2 and therefore constitutes a useful tool to study the SOX2 expression dynamics upon hiPSC culture, differentiation and somatic cell reprogramming." @default.
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- W2616302590 date "2017-07-01" @default.
- W2616302590 modified "2023-09-29" @default.
- W2616302590 title "Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9" @default.
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- W2616302590 doi "https://doi.org/10.1016/j.scr.2017.05.005" @default.
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