FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2616747577> ?p ?o ?g. }

• W2616747577 endingPage "1778" @default.
• W2616747577 startingPage "1764" @default.
• W2616747577 abstract "In plants, microRNA (miRNA)-target complementarity has long been considered the predominant factor determining the silencing outcome of the miRNA-target interaction, although the efficacy of such interactions have rarely been appraised in plants. Here, we perform in planta silencing efficacy assays on seven Arabidopsis MYB genes, all of which contain conserved miR159-binding sites of analogous complementarity. These genes were found to be differentially silenced by miR159; MYB81, MYB97, MYB101, MYB104, and DUO1 were all poorly silenced, whereas MYB33 and MYB65 were strongly silenced. Curiously, this is consistent with previous genetic analysis defining MYB33 and MYB65 as the major functional targets of miR159. Neither the free energy of miR159-target complementarity, nor miRNA binding site accessibility, as determined by flanking region AU content, could fully explain the discrepancy of miR159 silencing efficacy. Instead, we found that MYB33 and MYB65 were both predicted to contain a distinctive RNA secondary structure abutting the miR159 binding site. The structure is composed of two stem-loops (SLs) that are predicted to form in MYB33/65 homologs of species as evolutionary distant as gymnosperms. Functional analysis found that the RNA structure in MYB33 correlated with strong silencing efficacy; introducing mutations to disrupt either SL attenuated miR159 efficacy, while introducing complementary mutations to restore the SLs, but not the sequence, restored strong miR159-mediated silencing. Therefore, it appears that this RNA secondary structure demarcates MYB33/65 as sensitive targets of miR159, which underpins the narrow functional specificity of Arabidopsis miR159." @default.
• W2616747577 created "2017-05-26" @default.
• W2616747577 creator A5010712606 @default.
• W2616747577 creator A5018152109 @default.
• W2616747577 creator A5023448224 @default.
• W2616747577 creator A5034729561 @default.
• W2616747577 creator A5038759115 @default.
• W2616747577 creator A5082250184 @default.
• W2616747577 date "2017-05-17" @default.
• W2616747577 modified "2023-10-16" @default.
• W2616747577 title "Target RNA Secondary Structure Is a Major Determinant of miR159 Efficacy" @default.
• W2616747577 cites W1844751807 @default.
• W2616747577 cites W1971028992 @default.
• W2616747577 cites W1971739164 @default.
• W2616747577 cites W1982660543 @default.
• W2616747577 cites W1990876949 @default.
• W2616747577 cites W1992873592 @default.
• W2616747577 cites W1993533777 @default.
• W2616747577 cites W1997502251 @default.
• W2616747577 cites W2009777681 @default.
• W2616747577 cites W2018403717 @default.
• W2616747577 cites W2022441904 @default.
• W2616747577 cites W2030093783 @default.
• W2616747577 cites W2030632688 @default.
• W2616747577 cites W2044616519 @default.
• W2616747577 cites W2048728930 @default.
• W2616747577 cites W2051657543 @default.
• W2616747577 cites W2056504530 @default.
• W2616747577 cites W2056918383 @default.
• W2616747577 cites W2064098867 @default.
• W2616747577 cites W2082315179 @default.
• W2616747577 cites W2082885521 @default.
• W2616747577 cites W2090287641 @default.
• W2616747577 cites W2092061344 @default.
• W2616747577 cites W2116122104 @default.
• W2616747577 cites W2117207028 @default.
• W2616747577 cites W2118771503 @default.
• W2616747577 cites W2120772351 @default.
• W2616747577 cites W2122135986 @default.
• W2616747577 cites W2124276684 @default.
• W2616747577 cites W2125617003 @default.
• W2616747577 cites W2138296380 @default.
• W2616747577 cites W2144177541 @default.
• W2616747577 cites W2146100687 @default.
• W2616747577 cites W2149785901 @default.
• W2616747577 cites W2151753597 @default.
• W2616747577 cites W2155801118 @default.
• W2616747577 cites W2162170048 @default.
• W2616747577 cites W2163448850 @default.
• W2616747577 cites W2163705253 @default.
• W2616747577 cites W2167018551 @default.
• W2616747577 cites W2170561036 @default.
• W2616747577 cites W2172283476 @default.
• W2616747577 cites W2509408799 @default.
• W2616747577 cites W2560090610 @default.
• W2616747577 cites W986646378 @default.
• W2616747577 doi "https://doi.org/10.1104/pp.16.01898" @default.
• W2616747577 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5490886" @default.
• W2616747577 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28515145" @default.
• W2616747577 hasPublicationYear "2017" @default.
• W2616747577 type Work @default.
• W2616747577 sameAs 2616747577 @default.
• W2616747577 citedByCount "33" @default.
• W2616747577 countsByYear W26167475772017 @default.
• W2616747577 countsByYear W26167475772018 @default.
• W2616747577 countsByYear W26167475772019 @default.
• W2616747577 countsByYear W26167475772020 @default.
• W2616747577 countsByYear W26167475772021 @default.
• W2616747577 countsByYear W26167475772022 @default.
• W2616747577 countsByYear W26167475772023 @default.
• W2616747577 crossrefType "journal-article" @default.
• W2616747577 hasAuthorship W2616747577A5010712606 @default.
• W2616747577 hasAuthorship W2616747577A5018152109 @default.
• W2616747577 hasAuthorship W2616747577A5023448224 @default.
• W2616747577 hasAuthorship W2616747577A5034729561 @default.
• W2616747577 hasAuthorship W2616747577A5038759115 @default.
• W2616747577 hasAuthorship W2616747577A5082250184 @default.
• W2616747577 hasBestOaLocation W26167475771 @default.
• W2616747577 hasConcept C104317684 @default.
• W2616747577 hasConcept C119056186 @default.
• W2616747577 hasConcept C143065580 @default.
• W2616747577 hasConcept C145059251 @default.
• W2616747577 hasConcept C166703698 @default.
• W2616747577 hasConcept C202269582 @default.
• W2616747577 hasConcept C2191507 @default.
• W2616747577 hasConcept C2779491563 @default.
• W2616747577 hasConcept C54355233 @default.
• W2616747577 hasConcept C60723119 @default.
• W2616747577 hasConcept C62794011 @default.
• W2616747577 hasConcept C67705224 @default.
• W2616747577 hasConcept C70721500 @default.
• W2616747577 hasConcept C85468374 @default.
• W2616747577 hasConcept C86339819 @default.
• W2616747577 hasConcept C86803240 @default.
• W2616747577 hasConceptScore W2616747577C104317684 @default.
• W2616747577 hasConceptScore W2616747577C119056186 @default.
• W2616747577 hasConceptScore W2616747577C143065580 @default.
• W2616747577 hasConceptScore W2616747577C145059251 @default.

• next