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• W2616775339 abstract "Conventional respiratory tract specimens, such as bronchoalveolar lavage (BAL) fluid and induced sputum for diagnosing Pneumocystis jirovecii pneumonia (PCP) in immunocompromised patients are difficult to obtain. Besides, bronchoscopy is an invasive procedure that carries the risk of causing rapidly progressive respiratory insufficiency. By contrast, serum cell-free DNA (cfDNA) is easy to obtain and has been proven useful in diagnosing cancer, pregnancy associated complications, parasite infection and sepsis. In this study, we performed quantitative polymerase chain reaction (qPCR) to assess the diagnostic efficiency of using serum cfDNA, BAL fluid, and sputum DNA for PCP. Seventy-one patients (35 PCP patients and 36 non-PCP patients) were enrolled according to the clinical PCP diagnostic criteria. The sensitivity, specificity, positive predictive value, and negative predictive value of PCR using serum cfDNA were 68.6% (95% CI, 50.7-83.1), 97.2% (95% CI, 85.5-99.9), 96.0%, and 76.1%, respectively. PCR using BAL fluid and sputum had a high sensitivity (97.1% and 91.4%, respectively) but relatively low specificity (86.1% and 86.1%, respectively). The combination of the sputum PCR OR serum cfDNA PCR yielded a sensitivity of 97.1%.These results indicated that serum cfDNA might be a valuable method in PCP diagnosis." @default.
• W2616775339 created "2017-05-26" @default.
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• W2616775339 date "2017-05-20" @default.
• W2616775339 modified "2023-10-14" @default.
• W2616775339 title "Diagnosis of <i>Pneumocystis jirovecii</i> pneumonia with serum cell-free DNA in non-HIV-infected immunocompromised patients" @default.
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• W2616775339 doi "https://doi.org/10.18632/oncotarget.18037" @default.
• W2616775339 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5641102" @default.
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