FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2616998933> ?p ?o ?g. }

• W2616998933 abstract "Autophagy is a conserved catabolic process to degrade cellular organelles. The role of autophagy in cancer development is complex. Amplification of fibroblast growth factor receptor 1 (FGFR1) is one of the most frequent targets in lung squamous cell carcinoma (SQCC). Whether fibroblast growth factor 2 (FGF2)/FGFR1 contributes to the regulation of autophagy remains elusive.Autophagic activity was evaluated by immunoblotting for microtubule-associated protein 1 light chain 3 (LC3), formation of GFP-LC3 puncta, and monodansylcadaverine (MDC) staining. The effect of autophagy inhibition on cell survival was assessed by cell viability and apoptosis assays.We elucidated that FGFR1 activation suppressed autophagy. Pharmacological or genetic inhibition of FGFR1 by AZD4547 or FGFR1 short hairpin RNA (shRNA) induced autophagy in FGFR1-amplified non-small cell lung cancer (NSCLC) cells, H1581 and H520 cells. Mechanistic study revealed that the induction of autophagy by FGFR1 inhibition was mediated through inhibiting the ERK/MAPK pathway not by AKT pathway, accompanied by upregulation of beclin-1. Furthermore, activation of ERK/MAPK by transfection with a constitutively active MEK1 (caMEK1) construct or knockdown of beclin-1 by RNAi could attenuate autophagy induced by FGFR1 inhibition. Beclin-1 expression was inversely correlated with MEK1 phosphorylation. Inhibition of autophagy by beclin-1 silencing could enhance apoptosis after AZD4547 treatment in H1581 and H520 cells. High levels of LC3B mRNA was a marker of poor prognosis in NSCLC patients.Simultaneously inhibiting FGFR1 and autophagy could enhance cell death which should be further explored in vivo." @default.
• W2616998933 created "2017-06-05" @default.
• W2616998933 creator A5000283319 @default.
• W2616998933 creator A5004928583 @default.
• W2616998933 creator A5036365256 @default.
• W2616998933 creator A5040935769 @default.
• W2616998933 creator A5061273431 @default.
• W2616998933 creator A5062047914 @default.
• W2616998933 date "2017-05-30" @default.
• W2616998933 modified "2023-10-13" @default.
• W2616998933 title "FGF2/FGFR1 regulates autophagy in FGFR1-amplified non-small cell lung cancer cells" @default.
• W2616998933 cites W1523693806 @default.
• W2616998933 cites W1599366903 @default.
• W2616998933 cites W1784425676 @default.
• W2616998933 cites W1965228309 @default.
• W2616998933 cites W1977721235 @default.
• W2616998933 cites W1978103932 @default.
• W2616998933 cites W1982000972 @default.
• W2616998933 cites W1987769185 @default.
• W2616998933 cites W1994823455 @default.
• W2616998933 cites W2017016244 @default.
• W2616998933 cites W2017813632 @default.
• W2616998933 cites W2019576484 @default.
• W2616998933 cites W2019711215 @default.
• W2616998933 cites W2025665129 @default.
• W2616998933 cites W2030148643 @default.
• W2616998933 cites W2034132952 @default.
• W2616998933 cites W2044293524 @default.
• W2616998933 cites W2044596456 @default.
• W2616998933 cites W2048519887 @default.
• W2616998933 cites W2064746187 @default.
• W2616998933 cites W2065031087 @default.
• W2616998933 cites W2078054478 @default.
• W2616998933 cites W2079282879 @default.
• W2616998933 cites W2089074573 @default.
• W2616998933 cites W2089230333 @default.
• W2616998933 cites W2097299991 @default.
• W2616998933 cites W2105114667 @default.
• W2616998933 cites W2106465830 @default.
• W2616998933 cites W2110628016 @default.
• W2616998933 cites W2114843025 @default.
• W2616998933 cites W2115886998 @default.
• W2616998933 cites W2118696726 @default.
• W2616998933 cites W2121325716 @default.
• W2616998933 cites W2123388712 @default.
• W2616998933 cites W2125514375 @default.
• W2616998933 cites W2140331134 @default.
• W2616998933 cites W2146683945 @default.
• W2616998933 cites W2149441684 @default.
• W2616998933 cites W2149792725 @default.
• W2616998933 cites W2159060826 @default.
• W2616998933 cites W2159809160 @default.
• W2616998933 cites W2163832829 @default.
• W2616998933 cites W2173822655 @default.
• W2616998933 cites W2235523093 @default.
• W2616998933 cites W2275678839 @default.
• W2616998933 cites W2275877493 @default.
• W2616998933 cites W2291066865 @default.
• W2616998933 cites W2292721782 @default.
• W2616998933 cites W2299572076 @default.
• W2616998933 cites W2408352991 @default.
• W2616998933 cites W2541714644 @default.
• W2616998933 cites W2592030361 @default.
• W2616998933 cites W2602035017 @default.
• W2616998933 cites W2752326209 @default.
• W2616998933 cites W2778712486 @default.
• W2616998933 cites W97697689 @default.
• W2616998933 doi "https://doi.org/10.1186/s13046-017-0534-0" @default.
• W2616998933 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5450166" @default.
• W2616998933 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28558758" @default.
• W2616998933 hasPublicationYear "2017" @default.
• W2616998933 type Work @default.
• W2616998933 sameAs 2616998933 @default.
• W2616998933 citedByCount "42" @default.
• W2616998933 countsByYear W26169989332017 @default.
• W2616998933 countsByYear W26169989332018 @default.
• W2616998933 countsByYear W26169989332019 @default.
• W2616998933 countsByYear W26169989332020 @default.
• W2616998933 countsByYear W26169989332021 @default.
• W2616998933 countsByYear W26169989332022 @default.
• W2616998933 countsByYear W26169989332023 @default.
• W2616998933 crossrefType "journal-article" @default.
• W2616998933 hasAuthorship W2616998933A5000283319 @default.
• W2616998933 hasAuthorship W2616998933A5004928583 @default.
• W2616998933 hasAuthorship W2616998933A5036365256 @default.
• W2616998933 hasAuthorship W2616998933A5040935769 @default.
• W2616998933 hasAuthorship W2616998933A5061273431 @default.
• W2616998933 hasAuthorship W2616998933A5062047914 @default.
• W2616998933 hasBestOaLocation W26169989331 @default.
• W2616998933 hasConcept C104317684 @default.
• W2616998933 hasConcept C119056186 @default.
• W2616998933 hasConcept C170493617 @default.
• W2616998933 hasConcept C173396325 @default.
• W2616998933 hasConcept C185592680 @default.
• W2616998933 hasConcept C190232843 @default.
• W2616998933 hasConcept C190283241 @default.
• W2616998933 hasConcept C203522944 @default.
• W2616998933 hasConcept C49418065 @default.
• W2616998933 hasConcept C502942594 @default.
• W2616998933 hasConcept C54009773 @default.

• next