FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2617100209> ?p ?o ?g. }

• W2617100209 endingPage "280" @default.
• W2617100209 startingPage "269" @default.
• W2617100209 abstract "Cholangiocarcinoma (CCA), derived from the bile duct, occurs with a relatively high incidence in Northeast Thailand. Early diagnosis is still hampered by the lack of sufficient biomarkers. In recent years, biomarker discovery using secretomes has provided interesting results, including our studies on CCA secretomes, especially with three-dimensional cell cultures. Thus, cells cultured using the hollow fiber bioreactor (HFB) with 20 kDa molecular weight cut-off (MWCO) yielded higher quality and quantity of secretomes than those from conditioned media of the monolayer culture (MNC) system. In this study, we employed the HFB culture system with 5 kDa MWCO and compared conditioned media from the HFB and MNC systems using two-dimensional gel electrophoresis, followed by identifying proteins of interest by liquid chromatography and mass spectrometry (LC/MS/MS). Two out of 4 spots of NGAL or lipocalin-2 were found to show highest increase in expression of 19.93-fold and 18.79-fold in HFB compared to MNC. Interestingly, all 14 proteasome subunits including proteasome subunit α type-1 to type-7 and β type-1 to type-7 showed 2.92-fold to 12.13-fold increased expression in HFB. The protein-protein interactions of upregulated proteins were predicted, and one of the main interaction clusters involved 20S proteasome subunits. Proteasome activity in the HFB conditioned media was also found to be higher than that in MNC conditioned media. Three types of proteasome subunit were also validated by immunoblotting and showed higher expression in the HFB system compared to MNC system. Proteasome subunit α type-3 (PSMA3) showed the highest level in plasma of cholangiocarcinoma patients compared to normal and hepatocellular carcinoma patients by immunodetection, and is of interest as a potential biomarker for cholangiocarcinoma." @default.
• W2617100209 created "2017-06-05" @default.
• W2617100209 creator A5007940827 @default.
• W2617100209 creator A5018123020 @default.
• W2617100209 creator A5042384881 @default.
• W2617100209 creator A5047182592 @default.
• W2617100209 creator A5061687760 @default.
• W2617100209 creator A5069503570 @default.
• W2617100209 creator A5072469061 @default.
• W2617100209 creator A5072662684 @default.
• W2617100209 creator A5072810567 @default.
• W2617100209 creator A5073343849 @default.
• W2617100209 creator A5079174819 @default.
• W2617100209 creator A5086096239 @default.
• W2617100209 date "2017-05-29" @default.
• W2617100209 modified "2023-09-27" @default.
• W2617100209 title "Secretomic profiling of cells from hollow fiber bioreactor reveals PSMA3 as a potential cholangiocarcinoma biomarker" @default.
• W2617100209 cites W1581920763 @default.
• W2617100209 cites W1669342046 @default.
• W2617100209 cites W1866293606 @default.
• W2617100209 cites W1964963501 @default.
• W2617100209 cites W1965191255 @default.
• W2617100209 cites W1966900644 @default.
• W2617100209 cites W1968924273 @default.
• W2617100209 cites W1974973209 @default.
• W2617100209 cites W1979683878 @default.
• W2617100209 cites W1982302571 @default.
• W2617100209 cites W1989085472 @default.
• W2617100209 cites W1989812511 @default.
• W2617100209 cites W1991043006 @default.
• W2617100209 cites W2000465242 @default.
• W2617100209 cites W2004783639 @default.
• W2617100209 cites W2010869804 @default.
• W2617100209 cites W2011457559 @default.
• W2617100209 cites W2027159479 @default.
• W2617100209 cites W2038118774 @default.
• W2617100209 cites W2046928159 @default.
• W2617100209 cites W2052001271 @default.
• W2617100209 cites W2059694525 @default.
• W2617100209 cites W2069523698 @default.
• W2617100209 cites W2084403164 @default.
• W2617100209 cites W2089286192 @default.
• W2617100209 cites W2102666037 @default.
• W2617100209 cites W2104738007 @default.
• W2617100209 cites W2110256992 @default.
• W2617100209 cites W2118937204 @default.
• W2617100209 cites W2121589581 @default.
• W2617100209 cites W2128884833 @default.
• W2617100209 cites W2135972280 @default.
• W2617100209 cites W2152643523 @default.
• W2617100209 cites W2158971892 @default.
• W2617100209 cites W2159818270 @default.
• W2617100209 cites W2161249616 @default.
• W2617100209 cites W2167219679 @default.
• W2617100209 cites W2335231316 @default.
• W2617100209 cites W2541964584 @default.
• W2617100209 doi "https://doi.org/10.3892/ijo.2017.4024" @default.
• W2617100209 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28560424" @default.
• W2617100209 hasPublicationYear "2017" @default.
• W2617100209 type Work @default.
• W2617100209 sameAs 2617100209 @default.
• W2617100209 citedByCount "6" @default.
• W2617100209 countsByYear W26171002092019 @default.
• W2617100209 countsByYear W26171002092021 @default.
• W2617100209 countsByYear W26171002092022 @default.
• W2617100209 crossrefType "journal-article" @default.
• W2617100209 hasAuthorship W2617100209A5007940827 @default.
• W2617100209 hasAuthorship W2617100209A5018123020 @default.
• W2617100209 hasAuthorship W2617100209A5042384881 @default.
• W2617100209 hasAuthorship W2617100209A5047182592 @default.
• W2617100209 hasAuthorship W2617100209A5061687760 @default.
• W2617100209 hasAuthorship W2617100209A5069503570 @default.
• W2617100209 hasAuthorship W2617100209A5072469061 @default.
• W2617100209 hasAuthorship W2617100209A5072662684 @default.
• W2617100209 hasAuthorship W2617100209A5072810567 @default.
• W2617100209 hasAuthorship W2617100209A5073343849 @default.
• W2617100209 hasAuthorship W2617100209A5079174819 @default.
• W2617100209 hasAuthorship W2617100209A5086096239 @default.
• W2617100209 hasBestOaLocation W26171002091 @default.
• W2617100209 hasConcept C104292427 @default.
• W2617100209 hasConcept C104317684 @default.
• W2617100209 hasConcept C116084860 @default.
• W2617100209 hasConcept C153911025 @default.
• W2617100209 hasConcept C185592680 @default.
• W2617100209 hasConcept C27740335 @default.
• W2617100209 hasConcept C2781197716 @default.
• W2617100209 hasConcept C54355233 @default.
• W2617100209 hasConcept C55493867 @default.
• W2617100209 hasConcept C81885089 @default.
• W2617100209 hasConcept C86803240 @default.
• W2617100209 hasConceptScore W2617100209C104292427 @default.
• W2617100209 hasConceptScore W2617100209C104317684 @default.
• W2617100209 hasConceptScore W2617100209C116084860 @default.
• W2617100209 hasConceptScore W2617100209C153911025 @default.
• W2617100209 hasConceptScore W2617100209C185592680 @default.
• W2617100209 hasConceptScore W2617100209C27740335 @default.
• W2617100209 hasConceptScore W2617100209C2781197716 @default.
• W2617100209 hasConceptScore W2617100209C54355233 @default.

• next