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- W2617242788 abstract "Metallochaperones are responsible for shuttling metal ions to target proteins. Thus, a metallochaperone's structure must be sufficiently flexible both to hold onto its ion while traversing the cytoplasm and to transfer the ion to or from a partner protein. Here, we sought to shed light on the structure of Atox1, a metallochaperone involved in the human copper regulation system. Atox1 shuttles copper ions from the main copper transporter, Ctr1, to the ATP7b transporter in the Golgi apparatus. Conventional biophysical tools such as X-ray or NMR cannot always target the various conformational states of metallochaperones, owing to a requirement for crystallography or low sensitivity and resolution. Electron paramagnetic resonance (EPR) spectroscopy has recently emerged as a powerful tool for resolving biological reactions and mechanisms in solution. When coupled with computational methods, EPR with site-directed spin labeling and nanoscale distance measurements can provide structural information on a protein or protein complex in solution. We use these methods to show that Atox1 can accommodate at least four different conformations in the apo state (unbound to copper), and two different conformations in the holo state (bound to copper). We also demonstrate that the structure of Atox1 in the holo form is more compact than in the apo form. Our data provide insight regarding the structural mechanisms through which Atox1 can fulfill its dual role of copper binding and transfer." @default.
- W2617242788 created "2017-06-05" @default.
- W2617242788 creator A5058721478 @default.
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- W2617242788 date "2017-05-31" @default.
- W2617242788 modified "2023-10-17" @default.
- W2617242788 title "The structural flexibility of the human copper chaperone Atox1: Insights from combined pulsed EPR studies and computations" @default.
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- W2617242788 doi "https://doi.org/10.1002/pro.3197" @default.
- W2617242788 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5521546" @default.
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- W2617242788 hasPublicationYear "2017" @default.
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