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• W2617366712 abstract "http://dx.doi.org/10.5007/2175-7925.2017v30n2p1O objetivo deste estudo foi avaliar funcionalmente a influência do composto plumbagin sobre a indução heteróloga de uma região promotora predita de genes paraquat-induzíveis revelada durante as análises de anotação do genoma da bactéria Chromobacterium violaceum. A região promotora de interesse de C. violaceum foi amplificada a partir de sequências oligonucleotídicas específicas e clonada em vetor conjugativo, sendo acoplada à região codificante do gene lacZ de Escherichia coli. Em seguida, a indução heteróloga desse segmento regulatório foi estimada em cepa de E. coli na presença do plumbagin em uma concentração final de 50 µg/mL por mensurações dos níveis de expressão da enzima β-galactosidase. Diferenças significativas nos níveis da β-galactosidase foram observadas como resultado da ativação da região promotora de interesse pelo plumbagin na concentração testada em comparação às condições controle. Por outro lado, nenhum crescimento da cepa selvagem de C. violaceum foi observado durante a incubação dessas células em meio nutritivo contendo diferentes concentrações do plumbagin. As descobertas descritas aqui sugerem que uma região promotora predita para genes paraquat-induzíveis da bactéria C. violaceum sofra indução heteróloga pelo plumbagin, reforçando evidências acerca da caracterização funcional de motivos regulatórios intrínsecos a essa bactéria." @default.
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• W2617366712 date "2017-05-25" @default.
• W2617366712 modified "2023-09-30" @default.
• W2617366712 title "Heterologous expression of predicted promoter site for paraquat-inducible genes of the bacterium Chromobacterium violaceum is increased by plumbagin" @default.
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• W2617366712 doi "https://doi.org/10.5007/2175-7925.2017v30n2p1" @default.
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