Matches in SemOpenAlex for { <https://semopenalex.org/work/W2617502597> ?p ?o ?g. }
- W2617502597 endingPage "1528" @default.
- W2617502597 startingPage "1520" @default.
- W2617502597 abstract "Snakin‐1 (SN‐1) is a cysteine‐rich plant antimicrobial peptide and the first purified member of the snakin family. SN‐1 shows potent activity against a wide range of microorganisms, and thus has great biotechnological potential as an antimicrobial agent. Here, we produced recombinant SN‐1 in Escherichia coli by a previously developed coexpression method using an aggregation‐prone partner protein. Our goal was to increase the productivity of SN‐1 via the enhanced formation of insoluble inclusion bodies in E. coli cells. The yield of SN‐1 by the coexpression method was better than that by direct expression in E. coli cells. After refolding and purification, we obtained several milligrams of functionally active SN‐1, the identity of which was verified by MALDI‐TOF MS and NMR studies. The purified recombinant SN‐1 showed effective antimicrobial activity against test organisms. Our studies indicate that the coexpression method using an aggregation‐prone partner protein can serve as a suitable expression system for the efficient production of functionally active SN‐1. © 2017 American Institute of Chemical Engineers Biotechnol. Prog. , 33:1520–1528, 2017" @default.
- W2617502597 created "2017-06-05" @default.
- W2617502597 creator A5021912526 @default.
- W2617502597 creator A5028244090 @default.
- W2617502597 creator A5057541087 @default.
- W2617502597 creator A5076806518 @default.
- W2617502597 creator A5088394096 @default.
- W2617502597 creator A5088528790 @default.
- W2617502597 date "2017-06-12" @default.
- W2617502597 modified "2023-10-18" @default.
- W2617502597 title "Enhanced expression of cysteine‐rich antimicrobial peptide snakin‐1 in <i>Escherichia coli</i> using an aggregation‐prone protein coexpression system" @default.
- W2617502597 cites W1570190618 @default.
- W2617502597 cites W1797763128 @default.
- W2617502597 cites W1935851041 @default.
- W2617502597 cites W1965334087 @default.
- W2617502597 cites W1971071753 @default.
- W2617502597 cites W1980418552 @default.
- W2617502597 cites W1983605023 @default.
- W2617502597 cites W1986388841 @default.
- W2617502597 cites W1987254408 @default.
- W2617502597 cites W1990497071 @default.
- W2617502597 cites W1995783458 @default.
- W2617502597 cites W2008993701 @default.
- W2617502597 cites W2020182869 @default.
- W2617502597 cites W2023582179 @default.
- W2617502597 cites W2026050283 @default.
- W2617502597 cites W2032088945 @default.
- W2617502597 cites W2035167810 @default.
- W2617502597 cites W2042348092 @default.
- W2617502597 cites W2052409338 @default.
- W2617502597 cites W2054602599 @default.
- W2617502597 cites W2055347511 @default.
- W2617502597 cites W2060764828 @default.
- W2617502597 cites W2066692429 @default.
- W2617502597 cites W2075929383 @default.
- W2617502597 cites W2081687853 @default.
- W2617502597 cites W2113196743 @default.
- W2617502597 cites W2115251993 @default.
- W2617502597 cites W2127097337 @default.
- W2617502597 cites W2139217585 @default.
- W2617502597 cites W2141721821 @default.
- W2617502597 cites W2144306530 @default.
- W2617502597 cites W2169840340 @default.
- W2617502597 cites W2170364423 @default.
- W2617502597 cites W2171512559 @default.
- W2617502597 cites W2180208960 @default.
- W2617502597 cites W222333778 @default.
- W2617502597 cites W2277912195 @default.
- W2617502597 cites W2346659251 @default.
- W2617502597 doi "https://doi.org/10.1002/btpr.2508" @default.
- W2617502597 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28556600" @default.
- W2617502597 hasPublicationYear "2017" @default.
- W2617502597 type Work @default.
- W2617502597 sameAs 2617502597 @default.
- W2617502597 citedByCount "9" @default.
- W2617502597 countsByYear W26175025972017 @default.
- W2617502597 countsByYear W26175025972018 @default.
- W2617502597 countsByYear W26175025972019 @default.
- W2617502597 countsByYear W26175025972020 @default.
- W2617502597 countsByYear W26175025972021 @default.
- W2617502597 countsByYear W26175025972022 @default.
- W2617502597 countsByYear W26175025972023 @default.
- W2617502597 crossrefType "journal-article" @default.
- W2617502597 hasAuthorship W2617502597A5021912526 @default.
- W2617502597 hasAuthorship W2617502597A5028244090 @default.
- W2617502597 hasAuthorship W2617502597A5057541087 @default.
- W2617502597 hasAuthorship W2617502597A5076806518 @default.
- W2617502597 hasAuthorship W2617502597A5088394096 @default.
- W2617502597 hasAuthorship W2617502597A5088528790 @default.
- W2617502597 hasBestOaLocation W26175025972 @default.
- W2617502597 hasConcept C104317684 @default.
- W2617502597 hasConcept C181199279 @default.
- W2617502597 hasConcept C185592680 @default.
- W2617502597 hasConcept C202751555 @default.
- W2617502597 hasConcept C203750385 @default.
- W2617502597 hasConcept C2779201268 @default.
- W2617502597 hasConcept C2779281246 @default.
- W2617502597 hasConcept C2780430158 @default.
- W2617502597 hasConcept C40767141 @default.
- W2617502597 hasConcept C4937899 @default.
- W2617502597 hasConcept C540938839 @default.
- W2617502597 hasConcept C547475151 @default.
- W2617502597 hasConcept C55493867 @default.
- W2617502597 hasConcept C86803240 @default.
- W2617502597 hasConcept C89423630 @default.
- W2617502597 hasConceptScore W2617502597C104317684 @default.
- W2617502597 hasConceptScore W2617502597C181199279 @default.
- W2617502597 hasConceptScore W2617502597C185592680 @default.
- W2617502597 hasConceptScore W2617502597C202751555 @default.
- W2617502597 hasConceptScore W2617502597C203750385 @default.
- W2617502597 hasConceptScore W2617502597C2779201268 @default.
- W2617502597 hasConceptScore W2617502597C2779281246 @default.
- W2617502597 hasConceptScore W2617502597C2780430158 @default.
- W2617502597 hasConceptScore W2617502597C40767141 @default.
- W2617502597 hasConceptScore W2617502597C4937899 @default.
- W2617502597 hasConceptScore W2617502597C540938839 @default.
- W2617502597 hasConceptScore W2617502597C547475151 @default.
- W2617502597 hasConceptScore W2617502597C55493867 @default.