Matches in SemOpenAlex for { <https://semopenalex.org/work/W2617676106> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2617676106 endingPage "76" @default.
- W2617676106 startingPage "70" @default.
- W2617676106 abstract "The isolation of good quality genomic DNA from formalin-fixed, paraffin-embedded tissues is challenging, especially in cases of small tissue specimens. The aim of our study was to evaluate a DNA extraction protocol using formalin-fixed, paraffin-embedded tissues in our laboratory and apply this method to a series of papillary thyroid microcarcinomas (PTMCs). A total of 25 PTMCs and 3 papillary thyroid carcinoma control cases were included in the study. We assessed a DNA extraction protocol on the basis of a precipitation method (MasterPure DNA purification kit, Epicentre), according to the manufacturer's instructions. All PTMCs were subject to real-time polymerase chain reaction (PCR) amplification targeting the BRAF gene and a housekeeping gene (GAPDH). BRAF gene mutations were then assessed by high-resolution melting analysis and confirmed by sequencing of the PCR products. Using this extraction method, we produced good yields of DNA (mean concentration, 147.4±77.8 ng/µL), in addition to high levels of purity (mean A260/A280 ratio: 1.63±0.1). We successfully assessed the BRAF mutation status in 24 cases (16 BRAF-negative; 8 BRAF positive), although 1 case revealed an inconclusive pattern following high-resolution melting analysis and sequencing of the PCR products. We observed no differences in the tumor size (P=0.693), storage period of the tumor block (P=0.282), DNA concentration (P=0.243), DNA purity (P=0.458), CpGAPDH (P=0.173), or CpBRAF (P=0.217) values between the BRAF-mutated and nonmutated group of PTMCs. Our findings demonstrate the importance of a reliable, reproducible DNA extraction technique for efficient PCR amplification, uniformly applied to all cases in this study, regardless of the BRAF mutation status." @default.
- W2617676106 created "2017-06-05" @default.
- W2617676106 creator A5007578355 @default.
- W2617676106 creator A5021094520 @default.
- W2617676106 creator A5039101198 @default.
- W2617676106 creator A5039435699 @default.
- W2617676106 creator A5086027365 @default.
- W2617676106 date "2019-01-01" @default.
- W2617676106 modified "2023-10-03" @default.
- W2617676106 title "Evaluation of a DNA Extraction and Purification Protocol Using Archived Formalin-fixed Paraffin-embedded Tissues for BRAF Mutations Analysis in Papillary Thyroid Microcarcinomas" @default.
- W2617676106 cites W1965271384 @default.
- W2617676106 cites W1973036362 @default.
- W2617676106 cites W1978955748 @default.
- W2617676106 cites W1982715981 @default.
- W2617676106 cites W2004831310 @default.
- W2617676106 cites W2041140837 @default.
- W2617676106 cites W2049171090 @default.
- W2617676106 cites W2067959916 @default.
- W2617676106 cites W2071726080 @default.
- W2617676106 cites W2084051656 @default.
- W2617676106 cites W2086886569 @default.
- W2617676106 cites W2097403264 @default.
- W2617676106 cites W2129756027 @default.
- W2617676106 cites W2133408946 @default.
- W2617676106 cites W2139578514 @default.
- W2617676106 cites W2149730399 @default.
- W2617676106 cites W2160716539 @default.
- W2617676106 cites W2163188200 @default.
- W2617676106 cites W2168917479 @default.
- W2617676106 cites W2172237973 @default.
- W2617676106 cites W2264088196 @default.
- W2617676106 cites W2283755692 @default.
- W2617676106 cites W2296327633 @default.
- W2617676106 cites W3166377851 @default.
- W2617676106 doi "https://doi.org/10.1097/pai.0000000000000535" @default.
- W2617676106 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28549037" @default.
- W2617676106 hasPublicationYear "2019" @default.
- W2617676106 type Work @default.
- W2617676106 sameAs 2617676106 @default.
- W2617676106 citedByCount "3" @default.
- W2617676106 countsByYear W26176761062020 @default.
- W2617676106 countsByYear W26176761062021 @default.
- W2617676106 countsByYear W26176761062023 @default.
- W2617676106 crossrefType "journal-article" @default.
- W2617676106 hasAuthorship W2617676106A5007578355 @default.
- W2617676106 hasAuthorship W2617676106A5021094520 @default.
- W2617676106 hasAuthorship W2617676106A5039101198 @default.
- W2617676106 hasAuthorship W2617676106A5039435699 @default.
- W2617676106 hasAuthorship W2617676106A5086027365 @default.
- W2617676106 hasConcept C104317684 @default.
- W2617676106 hasConcept C150194340 @default.
- W2617676106 hasConcept C153911025 @default.
- W2617676106 hasConcept C17757408 @default.
- W2617676106 hasConcept C185592680 @default.
- W2617676106 hasConcept C2993294228 @default.
- W2617676106 hasConcept C33922130 @default.
- W2617676106 hasConcept C34372108 @default.
- W2617676106 hasConcept C49105822 @default.
- W2617676106 hasConcept C526584372 @default.
- W2617676106 hasConcept C54355233 @default.
- W2617676106 hasConcept C552990157 @default.
- W2617676106 hasConcept C78063203 @default.
- W2617676106 hasConcept C86803240 @default.
- W2617676106 hasConceptScore W2617676106C104317684 @default.
- W2617676106 hasConceptScore W2617676106C150194340 @default.
- W2617676106 hasConceptScore W2617676106C153911025 @default.
- W2617676106 hasConceptScore W2617676106C17757408 @default.
- W2617676106 hasConceptScore W2617676106C185592680 @default.
- W2617676106 hasConceptScore W2617676106C2993294228 @default.
- W2617676106 hasConceptScore W2617676106C33922130 @default.
- W2617676106 hasConceptScore W2617676106C34372108 @default.
- W2617676106 hasConceptScore W2617676106C49105822 @default.
- W2617676106 hasConceptScore W2617676106C526584372 @default.
- W2617676106 hasConceptScore W2617676106C54355233 @default.
- W2617676106 hasConceptScore W2617676106C552990157 @default.
- W2617676106 hasConceptScore W2617676106C78063203 @default.
- W2617676106 hasConceptScore W2617676106C86803240 @default.
- W2617676106 hasIssue "1" @default.
- W2617676106 hasLocation W26176761061 @default.
- W2617676106 hasLocation W26176761062 @default.
- W2617676106 hasOpenAccess W2617676106 @default.
- W2617676106 hasPrimaryLocation W26176761061 @default.
- W2617676106 hasRelatedWork W2072292348 @default.
- W2617676106 hasRelatedWork W2089380281 @default.
- W2617676106 hasRelatedWork W2146834312 @default.
- W2617676106 hasRelatedWork W2185219645 @default.
- W2617676106 hasRelatedWork W2269641782 @default.
- W2617676106 hasRelatedWork W2360506994 @default.
- W2617676106 hasRelatedWork W2382592496 @default.
- W2617676106 hasRelatedWork W2899733843 @default.
- W2617676106 hasRelatedWork W3001354753 @default.
- W2617676106 hasRelatedWork W2203821510 @default.
- W2617676106 hasVolume "27" @default.
- W2617676106 isParatext "false" @default.
- W2617676106 isRetracted "false" @default.
- W2617676106 magId "2617676106" @default.
- W2617676106 workType "article" @default.