FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2617869842> ?p ?o ?g. }

• W2617869842 endingPage "e2826" @default.
• W2617869842 startingPage "e2826" @default.
• W2617869842 abstract "The process of controlled cellular death known as apoptosis has an important central role not only in normal homeostatic maintenance of tissues, but also in numerous diseases such as cancer, neurodegenerative, autoimmune, and cardiovascular diseases. As a result, new technologies with the capability to selectively detect apoptotic cells represent a central focus of research for the study of these conditions. We have developed a new biosensor for the detection of apoptotic cells, incorporating the targeted selectivity for apoptotic cells from Annexin V with the sensitivity of bioluminescence signal generation from a serum-stable mutant of Renilla luciferase (RLuc8). Our data presents a complete characterization of the structural and biochemical properties of this new Annexin-Renilla fusion protein (ArFP) construct, as well as a validation of its ability to detect apoptosis in vitro. Moreover, this work represents the first report of a bioluminescent Annexin V apoptosis sensor utilized in vivo. With this new construct, we examine apoptosis within disease-relevant animal models of surgery-induced ischemia/reperfusion, corneal injury, and retinal cell death as a model of age-related macular degeneration. In each of these experiments, we demonstrate successful application of the ArFP construct for detection and bioluminescence imaging of apoptosis within each disease or treatment model. ArFP represents an important new tool in the continuously growing kit of technologies for apoptosis detection, and our results from both in vitro and in vivo experiments suggest a diverse range of potential clinically relevant applications including cancer therapeutic screening and efficacy analysis, atherosclerosis and cardiovascular disease detection, and the monitoring of any number of other conditions in which apoptosis has a central role." @default.
• W2617869842 created "2017-06-05" @default.
• W2617869842 creator A5001532670 @default.
• W2617869842 creator A5035031043 @default.
• W2617869842 creator A5052008284 @default.
• W2617869842 creator A5060293409 @default.
• W2617869842 creator A5075721340 @default.
• W2617869842 creator A5076922510 @default.
• W2617869842 creator A5084686245 @default.
• W2617869842 creator A5087651357 @default.
• W2617869842 date "2017-05-25" @default.
• W2617869842 modified "2023-10-10" @default.
• W2617869842 title "An enhanced bioluminescence-based Annexin V probe for apoptosis detection in vitro and in vivo" @default.
• W2617869842 cites W1560899257 @default.
• W2617869842 cites W1566186846 @default.
• W2617869842 cites W1568398137 @default.
• W2617869842 cites W1601217323 @default.
• W2617869842 cites W1871390418 @default.
• W2617869842 cites W1963651026 @default.
• W2617869842 cites W1965253898 @default.
• W2617869842 cites W1971742364 @default.
• W2617869842 cites W1976352504 @default.
• W2617869842 cites W1976998565 @default.
• W2617869842 cites W1977933482 @default.
• W2617869842 cites W1989062380 @default.
• W2617869842 cites W1989270637 @default.
• W2617869842 cites W2008757142 @default.
• W2617869842 cites W2025938842 @default.
• W2617869842 cites W2025984690 @default.
• W2617869842 cites W2026727321 @default.
• W2617869842 cites W2028064214 @default.
• W2617869842 cites W2036918052 @default.
• W2617869842 cites W2042141743 @default.
• W2617869842 cites W2049128343 @default.
• W2617869842 cites W2050957868 @default.
• W2617869842 cites W2052853635 @default.
• W2617869842 cites W2053669884 @default.
• W2617869842 cites W2056817309 @default.
• W2617869842 cites W2065711792 @default.
• W2617869842 cites W2066171290 @default.
• W2617869842 cites W2066694476 @default.
• W2617869842 cites W2069855944 @default.
• W2617869842 cites W2073831115 @default.
• W2617869842 cites W2073836632 @default.
• W2617869842 cites W2076104235 @default.
• W2617869842 cites W2078532650 @default.
• W2617869842 cites W2089933045 @default.
• W2617869842 cites W2098956821 @default.
• W2617869842 cites W2099133435 @default.
• W2617869842 cites W2113256330 @default.
• W2617869842 cites W2116285177 @default.
• W2617869842 cites W2119267802 @default.
• W2617869842 cites W2123021242 @default.
• W2617869842 cites W2128374973 @default.
• W2617869842 cites W2129723413 @default.
• W2617869842 cites W2157222011 @default.
• W2617869842 cites W2159875818 @default.
• W2617869842 cites W2166291746 @default.
• W2617869842 cites W2187458946 @default.
• W2617869842 cites W3103979403 @default.
• W2617869842 cites W4235504646 @default.
• W2617869842 cites W4251738959 @default.
• W2617869842 doi "https://doi.org/10.1038/cddis.2017.141" @default.
• W2617869842 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5520691" @default.
• W2617869842 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28542141" @default.
• W2617869842 hasPublicationYear "2017" @default.
• W2617869842 type Work @default.
• W2617869842 sameAs 2617869842 @default.
• W2617869842 citedByCount "21" @default.
• W2617869842 countsByYear W26178698422018 @default.
• W2617869842 countsByYear W26178698422019 @default.
• W2617869842 countsByYear W26178698422020 @default.
• W2617869842 countsByYear W26178698422021 @default.
• W2617869842 countsByYear W26178698422022 @default.
• W2617869842 crossrefType "journal-article" @default.
• W2617869842 hasAuthorship W2617869842A5001532670 @default.
• W2617869842 hasAuthorship W2617869842A5035031043 @default.
• W2617869842 hasAuthorship W2617869842A5052008284 @default.
• W2617869842 hasAuthorship W2617869842A5060293409 @default.
• W2617869842 hasAuthorship W2617869842A5075721340 @default.
• W2617869842 hasAuthorship W2617869842A5076922510 @default.
• W2617869842 hasAuthorship W2617869842A5084686245 @default.
• W2617869842 hasAuthorship W2617869842A5087651357 @default.
• W2617869842 hasBestOaLocation W26178698421 @default.
• W2617869842 hasConcept C111335760 @default.
• W2617869842 hasConcept C150903083 @default.
• W2617869842 hasConcept C161997846 @default.
• W2617869842 hasConcept C190283241 @default.
• W2617869842 hasConcept C202751555 @default.
• W2617869842 hasConcept C207001950 @default.
• W2617869842 hasConcept C2780670749 @default.
• W2617869842 hasConcept C31573885 @default.
• W2617869842 hasConcept C502942594 @default.
• W2617869842 hasConcept C5176359 @default.
• W2617869842 hasConcept C54009773 @default.
• W2617869842 hasConcept C54355233 @default.
• W2617869842 hasConcept C55493867 @default.
• W2617869842 hasConcept C81885089 @default.

• next