Matches in SemOpenAlex for { <https://semopenalex.org/work/W2618232217> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2618232217 endingPage "23" @default.
- W2618232217 startingPage "5" @default.
- W2618232217 abstract "Introduction. Fusarim are widely spread in forest nurseries. It is difficult to identify Fusarium species using microbiological methods. The goal of this study was to identify the micromycetes of Fusarium genus isolated from Pinus sylvestris and Picea abies seedlings and to develop a method of species identification for F. oxysporum, F. verticilliodes, and F. graminearum based on the restriction analysis of amplified ribosomal DNA. Objects and research methodology. The universal primers ITS1 and ITS4 were used in the study. A PCR-product of about 550 base pairs for each Fusarium species was generated. Three Fusarium species (F. oxysporum, F. verticilliodes, and F. graminearum) were identified by the molecular genetic analysis based on the sequence of ITS rDNA region. Since these fungi are difficult to distinguish by morphological markers, a simple method based on the digestion by restriction endonucleases of ITS1-5,8S-ITS2-28S region was developed. Five restriction endonucleases (AluI, BstMBI, BstHHI, BsuRI, and HpaII) were used for digestion of the studied DNA. Research results. Only HpaII and BstHHI allowed to get the unique RFLP banding patterns for each Fusarium species. When using the restrictase HpaII, a unique fragment over 440 bp for F. oxysporum and two fragments over 300 bp and 160 bp for F. verticilloides were identificated. The digestion of PCR fragments by using BstHHI enzyme revealed two specific fragments (300 bp and 280 bp) for F. oxysporum and two fragments (300 bp and 190 bp) for F. verticilloides. Conclusion. The PCR-RFLP analysis of the amplified ITS region was proved to be a useful molecular tool for identification of F. oxysporum, F. verticilliodes, and F. graminearum." @default.
- W2618232217 created "2017-06-05" @default.
- W2618232217 creator A5057771605 @default.
- W2618232217 date "2016-02-01" @default.
- W2618232217 modified "2023-10-14" @default.
- W2618232217 title "The Formation of Steppe and Protective Afforestation Basement: Theoretical and Applied Aspects" @default.
- W2618232217 doi "https://doi.org/10.15350/2306-2827.2016.2.5" @default.
- W2618232217 hasPublicationYear "2016" @default.
- W2618232217 type Work @default.
- W2618232217 sameAs 2618232217 @default.
- W2618232217 citedByCount "4" @default.
- W2618232217 countsByYear W26182322172018 @default.
- W2618232217 countsByYear W26182322172019 @default.
- W2618232217 countsByYear W26182322172022 @default.
- W2618232217 crossrefType "journal-article" @default.
- W2618232217 hasAuthorship W2618232217A5057771605 @default.
- W2618232217 hasBestOaLocation W26182322171 @default.
- W2618232217 hasConcept C104317684 @default.
- W2618232217 hasConcept C128319531 @default.
- W2618232217 hasConcept C150194340 @default.
- W2618232217 hasConcept C168007829 @default.
- W2618232217 hasConcept C178790620 @default.
- W2618232217 hasConcept C185592680 @default.
- W2618232217 hasConcept C190727270 @default.
- W2618232217 hasConcept C207812311 @default.
- W2618232217 hasConcept C2777162945 @default.
- W2618232217 hasConcept C2777563447 @default.
- W2618232217 hasConcept C2778867309 @default.
- W2618232217 hasConcept C2779430813 @default.
- W2618232217 hasConcept C49105822 @default.
- W2618232217 hasConcept C54355233 @default.
- W2618232217 hasConcept C552990157 @default.
- W2618232217 hasConcept C59822182 @default.
- W2618232217 hasConcept C86803240 @default.
- W2618232217 hasConcept C90132467 @default.
- W2618232217 hasConceptScore W2618232217C104317684 @default.
- W2618232217 hasConceptScore W2618232217C128319531 @default.
- W2618232217 hasConceptScore W2618232217C150194340 @default.
- W2618232217 hasConceptScore W2618232217C168007829 @default.
- W2618232217 hasConceptScore W2618232217C178790620 @default.
- W2618232217 hasConceptScore W2618232217C185592680 @default.
- W2618232217 hasConceptScore W2618232217C190727270 @default.
- W2618232217 hasConceptScore W2618232217C207812311 @default.
- W2618232217 hasConceptScore W2618232217C2777162945 @default.
- W2618232217 hasConceptScore W2618232217C2777563447 @default.
- W2618232217 hasConceptScore W2618232217C2778867309 @default.
- W2618232217 hasConceptScore W2618232217C2779430813 @default.
- W2618232217 hasConceptScore W2618232217C49105822 @default.
- W2618232217 hasConceptScore W2618232217C54355233 @default.
- W2618232217 hasConceptScore W2618232217C552990157 @default.
- W2618232217 hasConceptScore W2618232217C59822182 @default.
- W2618232217 hasConceptScore W2618232217C86803240 @default.
- W2618232217 hasConceptScore W2618232217C90132467 @default.
- W2618232217 hasIssue "2" @default.
- W2618232217 hasLocation W26182322171 @default.
- W2618232217 hasOpenAccess W2618232217 @default.
- W2618232217 hasPrimaryLocation W26182322171 @default.
- W2618232217 hasRelatedWork W1517004684 @default.
- W2618232217 hasRelatedWork W1985136302 @default.
- W2618232217 hasRelatedWork W1988777850 @default.
- W2618232217 hasRelatedWork W1993206475 @default.
- W2618232217 hasRelatedWork W2060550909 @default.
- W2618232217 hasRelatedWork W2095024353 @default.
- W2618232217 hasRelatedWork W2407061988 @default.
- W2618232217 hasRelatedWork W2419525987 @default.
- W2618232217 hasRelatedWork W2531739981 @default.
- W2618232217 hasRelatedWork W2534617626 @default.
- W2618232217 hasVolume "30" @default.
- W2618232217 isParatext "false" @default.
- W2618232217 isRetracted "false" @default.
- W2618232217 magId "2618232217" @default.
- W2618232217 workType "article" @default.