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- W2618268353 abstract "Cell synchronization is important for the analysis of molecular events involved in cell spreading and motility. Electrostatic interactions between cells and surfaces were investigated in order to synchronize the first step in cell adhesion. LimE-GFP marked Dictyostelium discoideum cells were used for fluorescent tracking of actin polymerization events. Oscillating LimE fluorescent peaks were observed for individual cells in standard phosphate buffer during spreading. At low ionic concentration (phosphate sucrose buffer 0.17 mM), cells levitate over the conductive surfaces (Indium Tin Oxide, ITO) due to electrostatic repulsion. An electrochemical device was designed in order to apply an overpotential pulse (+2.5 V/Ag,AgCl) during 0.1 s to the ITO surface. In these conditions, protons are produced by water oxidation, which reduce the ITO negative surface charge and thus, attracting the levitating cells simultaneously. Consequently, these irreversible contacts with the surface triggered the onset of cell spreading. For 37 from 47 studied cells (80%), successive fluorescent peaks appear, more or less regularly spaced in time, showing an oscillating actin polymerization activity. Remarkably, no maxima appeared before 7 s after the pulse application. Moreover, 29 cells from 37 (79%) had the first peak within 4 seconds interval, between 7.5 s and 11.5 s after the pulse. Therefore, we obtained synchronization of the spreading of a cell population for the first time thanks to an electrochemical method." @default.
- W2618268353 created "2017-06-05" @default.
- W2618268353 creator A5033614909 @default.
- W2618268353 date "2010-10-01" @default.
- W2618268353 modified "2023-09-27" @default.
- W2618268353 title "Electrochemical Synchronization of Cell Spreading on Solid Surfaces" @default.
- W2618268353 cites W1490324405 @default.
- W2618268353 cites W1491459594 @default.
- W2618268353 cites W1492775073 @default.
- W2618268353 cites W1536436988 @default.
- W2618268353 cites W1590619267 @default.
- W2618268353 cites W1595518633 @default.
- W2618268353 cites W1601031308 @default.
- W2618268353 cites W1626382598 @default.
- W2618268353 cites W1829275410 @default.
- W2618268353 cites W1916601007 @default.
- W2618268353 cites W1931993285 @default.
- W2618268353 cites W1937718714 @default.
- W2618268353 cites W1945671024 @default.
- W2618268353 cites W1963666590 @default.
- W2618268353 cites W1966263644 @default.
- W2618268353 cites W1966751543 @default.
- W2618268353 cites W1968826282 @default.
- W2618268353 cites W1969959009 @default.
- W2618268353 cites W1971847357 @default.
- W2618268353 cites W1975229535 @default.
- W2618268353 cites W1976669405 @default.
- W2618268353 cites W1978278141 @default.
- W2618268353 cites W1978496968 @default.
- W2618268353 cites W1979038578 @default.
- W2618268353 cites W1979089505 @default.
- W2618268353 cites W1979513617 @default.
- W2618268353 cites W1980356012 @default.
- W2618268353 cites W1980440689 @default.
- W2618268353 cites W1987139499 @default.
- W2618268353 cites W1988345904 @default.
- W2618268353 cites W1990548511 @default.
- W2618268353 cites W1993260486 @default.
- W2618268353 cites W1994186189 @default.
- W2618268353 cites W1998079495 @default.
- W2618268353 cites W1998791915 @default.
- W2618268353 cites W1999838269 @default.
- W2618268353 cites W2005149945 @default.
- W2618268353 cites W2008845367 @default.
- W2618268353 cites W2009092366 @default.
- W2618268353 cites W2012516444 @default.
- W2618268353 cites W2012518316 @default.
- W2618268353 cites W2014293907 @default.
- W2618268353 cites W2015500729 @default.
- W2618268353 cites W2018787020 @default.
- W2618268353 cites W2021673391 @default.
- W2618268353 cites W2022480536 @default.
- W2618268353 cites W2023193403 @default.
- W2618268353 cites W2028640392 @default.
- W2618268353 cites W2030041577 @default.
- W2618268353 cites W2032579724 @default.
- W2618268353 cites W2036057109 @default.
- W2618268353 cites W2037824461 @default.
- W2618268353 cites W2041248225 @default.
- W2618268353 cites W2045267564 @default.
- W2618268353 cites W2046153348 @default.
- W2618268353 cites W2046155579 @default.
- W2618268353 cites W2046414016 @default.
- W2618268353 cites W2049471883 @default.
- W2618268353 cites W2051839442 @default.
- W2618268353 cites W2055668916 @default.
- W2618268353 cites W2056263558 @default.
- W2618268353 cites W2060370047 @default.
- W2618268353 cites W2060505370 @default.
- W2618268353 cites W2064960939 @default.
- W2618268353 cites W2067326539 @default.
- W2618268353 cites W2068261191 @default.
- W2618268353 cites W2069075928 @default.
- W2618268353 cites W2069386014 @default.
- W2618268353 cites W2071650045 @default.
- W2618268353 cites W2073584744 @default.
- W2618268353 cites W2074308942 @default.
- W2618268353 cites W2074438101 @default.
- W2618268353 cites W2075443006 @default.
- W2618268353 cites W2076644108 @default.
- W2618268353 cites W2076925010 @default.
- W2618268353 cites W2079944376 @default.
- W2618268353 cites W2080463022 @default.
- W2618268353 cites W2081213113 @default.
- W2618268353 cites W2081266434 @default.
- W2618268353 cites W2082279421 @default.
- W2618268353 cites W2082591764 @default.
- W2618268353 cites W2082690651 @default.
- W2618268353 cites W2084312697 @default.
- W2618268353 cites W2084820791 @default.
- W2618268353 cites W2086003295 @default.
- W2618268353 cites W2086209116 @default.
- W2618268353 cites W2086884747 @default.
- W2618268353 cites W2087612333 @default.
- W2618268353 cites W2091787929 @default.
- W2618268353 cites W2092102154 @default.
- W2618268353 cites W2092623916 @default.
- W2618268353 cites W2096121060 @default.
- W2618268353 cites W2105416994 @default.
- W2618268353 cites W2109677752 @default.