FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2618510427> ?p ?o ?g. }

• W2618510427 abstract "Major drawbacks of the available treatment against Chagas disease (American trypanosomiasis) include its toxicity and therapeutic inefficiency in the chronic phase of the infection, which makes it a concern among neglected diseases. Therefore, the discovery of alternative drugs for treating chronic Chagas disease requires immediate action. In this work, we evaluated the mushroom Pleurotus salmoneostramineus in the search for potential antiparasitic compounds. Fruit bodies of the basidiomycete Pleurotus salmoneostramineus were triturated and submitted to organic solvent extraction. After liquid-liquid partition of the crude extract, three fractions were obtained and the bioguided fractionation study was conducted to isolate the active metabolites. The elucidation of the chemical structure was performed using GC-MS and NMR techniques. The biological assays for antiparasitic activity were carried out using trypomastigotes of Trypanosoma cruzi and murine macrophages for mammalian cytotoxicity. The mechanism of action of the isolated compound used different fluorescent probes to evaluate the plasma membrane permeability, the potential of the mitochondrial membrane and the intracellular levels of reactive oxygen species (ROS). The most abundant fraction showing the antiparasitic activity was isolated and chemically elucidated, confirming the presence of ergosterol. It showed anti-Trypanosoma cruzi activity against trypomastigotes, with an IC50 value of 51.3 μg/mL. The compound demonstrated no cytotoxicity against mammalian cells to the maximal tested concentration of 200 μg/mL. The mechanism of action of ergosterol in Trypanosoma cruzi trypomastigotes resulted in permeabilization of the plasma membrane, as well as depolarization of mitochondrial membrane potential, leading to parasite death. Nevertheless, no increase in ROS levels could be observed, suggesting damages to plasma membrane rather than an induction of oxidative stress in the parasite. The selection of naturally antiparasitic secondary metabolites in basidiomycetes, such as ergosterol, may provide potential scaffolds for drug design studies against neglected diseases." @default.
• W2618510427 created "2017-06-05" @default.
• W2618510427 creator A5005286462 @default.
• W2618510427 creator A5006087404 @default.
• W2618510427 creator A5008833161 @default.
• W2618510427 creator A5017793275 @default.
• W2618510427 creator A5029305138 @default.
• W2618510427 creator A5039634567 @default.
• W2618510427 creator A5066237562 @default.
• W2618510427 creator A5070236530 @default.
• W2618510427 creator A5091423690 @default.
• W2618510427 date "2017-05-30" @default.
• W2618510427 modified "2023-10-02" @default.
• W2618510427 title "Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects Trypanosoma cruzi plasma membrane and mitochondria" @default.
• W2618510427 cites W1744965277 @default.
• W2618510427 cites W1886166638 @default.
• W2618510427 cites W1902848540 @default.
• W2618510427 cites W1967894072 @default.
• W2618510427 cites W1977903050 @default.
• W2618510427 cites W1987841426 @default.
• W2618510427 cites W1993841012 @default.
• W2618510427 cites W1995682977 @default.
• W2618510427 cites W1995734836 @default.
• W2618510427 cites W1997545346 @default.
• W2618510427 cites W2001361831 @default.
• W2618510427 cites W2012676222 @default.
• W2618510427 cites W2013953785 @default.
• W2618510427 cites W2014387796 @default.
• W2618510427 cites W2019562067 @default.
• W2618510427 cites W2021170451 @default.
• W2618510427 cites W2025113010 @default.
• W2618510427 cites W2030192015 @default.
• W2618510427 cites W2032170226 @default.
• W2618510427 cites W2032754817 @default.
• W2618510427 cites W2035609779 @default.
• W2618510427 cites W2036084302 @default.
• W2618510427 cites W2041713443 @default.
• W2618510427 cites W2046623416 @default.
• W2618510427 cites W2057359540 @default.
• W2618510427 cites W2058743119 @default.
• W2618510427 cites W2062000827 @default.
• W2618510427 cites W2069350228 @default.
• W2618510427 cites W2084889403 @default.
• W2618510427 cites W2085995029 @default.
• W2618510427 cites W2087632839 @default.
• W2618510427 cites W2090323152 @default.
• W2618510427 cites W2097440656 @default.
• W2618510427 cites W2100842539 @default.
• W2618510427 cites W2101015942 @default.
• W2618510427 cites W2102919060 @default.
• W2618510427 cites W2102967584 @default.
• W2618510427 cites W2108526120 @default.
• W2618510427 cites W2116191646 @default.
• W2618510427 cites W2120348159 @default.
• W2618510427 cites W2125888372 @default.
• W2618510427 cites W2127520128 @default.
• W2618510427 cites W2131683232 @default.
• W2618510427 cites W2133380971 @default.
• W2618510427 cites W2140716633 @default.
• W2618510427 cites W2147529679 @default.
• W2618510427 cites W2147897792 @default.
• W2618510427 cites W2156542647 @default.
• W2618510427 cites W2159340682 @default.
• W2618510427 cites W2167100279 @default.
• W2618510427 cites W2264945539 @default.
• W2618510427 cites W2399136921 @default.
• W2618510427 cites W2589886788 @default.
• W2618510427 doi "https://doi.org/10.1186/s40409-017-0120-0" @default.
• W2618510427 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5450401" @default.
• W2618510427 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28572816" @default.
• W2618510427 hasPublicationYear "2017" @default.
• W2618510427 type Work @default.
• W2618510427 sameAs 2618510427 @default.
• W2618510427 citedByCount "23" @default.
• W2618510427 countsByYear W26185104272018 @default.
• W2618510427 countsByYear W26185104272019 @default.
• W2618510427 countsByYear W26185104272020 @default.
• W2618510427 countsByYear W26185104272021 @default.
• W2618510427 countsByYear W26185104272022 @default.
• W2618510427 countsByYear W26185104272023 @default.
• W2618510427 crossrefType "journal-article" @default.
• W2618510427 hasAuthorship W2618510427A5005286462 @default.
• W2618510427 hasAuthorship W2618510427A5006087404 @default.
• W2618510427 hasAuthorship W2618510427A5008833161 @default.
• W2618510427 hasAuthorship W2618510427A5017793275 @default.
• W2618510427 hasAuthorship W2618510427A5029305138 @default.
• W2618510427 hasAuthorship W2618510427A5039634567 @default.
• W2618510427 hasAuthorship W2618510427A5066237562 @default.
• W2618510427 hasAuthorship W2618510427A5070236530 @default.
• W2618510427 hasAuthorship W2618510427A5091423690 @default.
• W2618510427 hasBestOaLocation W26185104271 @default.
• W2618510427 hasConcept C109316439 @default.
• W2618510427 hasConcept C136764020 @default.
• W2618510427 hasConcept C142724271 @default.
• W2618510427 hasConcept C159047783 @default.
• W2618510427 hasConcept C185592680 @default.
• W2618510427 hasConcept C202751555 @default.
• W2618510427 hasConcept C2776171842 @default.
• W2618510427 hasConcept C2777625466 @default.
• W2618510427 hasConcept C2777910421 @default.

• next