Matches in SemOpenAlex for { <https://semopenalex.org/work/W2620066011> ?p ?o ?g. }
- W2620066011 endingPage "e0178340" @default.
- W2620066011 startingPage "e0178340" @default.
- W2620066011 abstract "Objectives Preeclampsia is one of the main contributers to maternal and fetal morbidity and mortality during pregnancy. A history of preeclampsia puts mother and offspring at an increased cardiovascular risk in later life. We hypothesized that at the time of birth functional impairments of fetal endothelial cells can be detected in pregnancies complicated by preeclampsia and that a therapeutic intervention using 1,25 (OH)2 vitamin D3 can reverse the adverse effects of preeclampsia on cell function. Methods Human umbilical vein endothelial cells (HUVEC) were isolated from umbilical cords obtained from preeclamptic (N = 12) and uncomplicated pregnancies (N = 13, control). Placental villous tissue fragments from uncomplicated term pregnancies were incubated in explant culture for 48 h at 2% (hypoxia), 8% or 21% O2. Explant conditioned media (CM) was collected and pooled according to oxygen level. We compared the ability of preeclampsia vs. control HUVEC to migrate, proliferate, and form tubule-like networks in a Matrigel assay, in the presence/absence of CM and 1,25(OH)2 vitamin D3. Results HUVEC from preeclamptic pregnancies showed reduced migration (P = 0.04) and tubule formation (P = 0.04), but no change in proliferation (P = 0.16) compared to healthy pregnancies. Placental villous explant CM derived from 2% O2 incubations significantly reduced HUVEC migration, when compared to non-CM (P = 0.04). Vitamin D3 improved HUVEC function in neither of the groups. There was no significant difference in VEGF gene expression between healthy and preeclamptic pregnancies and no effect of Vitamin D3 on VEGF expression. Conclusions Reduced functional abilities of fetal endothelial cells from preeclamptic pregnancies suggests that disease pathways, possibly originating from the dysfunctional placenta, negatively impact fetal endothelium. The neutral effect of 1,25(OH)2 vitamin D3 contrasts with previous findings that vitamin D rescues the poor migration, proliferation and tubule formation exhibited by cord blood fetal endothelial progenitor cells from preeclamptic pregnancies. Further investigations to distinguish pathways by which offspring exposed to preeclampsia are at risk for cardiovascular disease are needed." @default.
- W2620066011 created "2017-06-05" @default.
- W2620066011 creator A5008233606 @default.
- W2620066011 creator A5027385851 @default.
- W2620066011 creator A5028469537 @default.
- W2620066011 creator A5034204040 @default.
- W2620066011 creator A5035837751 @default.
- W2620066011 date "2017-05-23" @default.
- W2620066011 modified "2023-09-27" @default.
- W2620066011 title "Impaired functional capacity of fetal endothelial cells in preeclampsia" @default.
- W2620066011 cites W125935861 @default.
- W2620066011 cites W1557910385 @default.
- W2620066011 cites W1968785526 @default.
- W2620066011 cites W1971895770 @default.
- W2620066011 cites W1976291192 @default.
- W2620066011 cites W1978193308 @default.
- W2620066011 cites W1979397042 @default.
- W2620066011 cites W1981620045 @default.
- W2620066011 cites W1985178609 @default.
- W2620066011 cites W1993175090 @default.
- W2620066011 cites W1993925802 @default.
- W2620066011 cites W1995901075 @default.
- W2620066011 cites W2002144803 @default.
- W2620066011 cites W2002408287 @default.
- W2620066011 cites W2005848269 @default.
- W2620066011 cites W2014894250 @default.
- W2620066011 cites W2019997266 @default.
- W2620066011 cites W2023976307 @default.
- W2620066011 cites W2034228182 @default.
- W2620066011 cites W2042344849 @default.
- W2620066011 cites W2049740593 @default.
- W2620066011 cites W2049887195 @default.
- W2620066011 cites W2059147235 @default.
- W2620066011 cites W2059277748 @default.
- W2620066011 cites W2063800030 @default.
- W2620066011 cites W2064057106 @default.
- W2620066011 cites W2064540829 @default.
- W2620066011 cites W2070508259 @default.
- W2620066011 cites W2073371406 @default.
- W2620066011 cites W2090351798 @default.
- W2620066011 cites W2092353813 @default.
- W2620066011 cites W2095217306 @default.
- W2620066011 cites W2103684777 @default.
- W2620066011 cites W2106849785 @default.
- W2620066011 cites W2124543571 @default.
- W2620066011 cites W2129206669 @default.
- W2620066011 cites W2133417600 @default.
- W2620066011 cites W2143180984 @default.
- W2620066011 cites W2145489993 @default.
- W2620066011 cites W2151960759 @default.
- W2620066011 cites W2155430176 @default.
- W2620066011 cites W2157603347 @default.
- W2620066011 cites W2158615121 @default.
- W2620066011 cites W2159272489 @default.
- W2620066011 cites W2167714900 @default.
- W2620066011 cites W2169208315 @default.
- W2620066011 cites W2269603020 @default.
- W2620066011 cites W2294708990 @default.
- W2620066011 cites W2357868452 @default.
- W2620066011 cites W2727742003 @default.
- W2620066011 cites W2885878352 @default.
- W2620066011 cites W3160565403 @default.
- W2620066011 doi "https://doi.org/10.1371/journal.pone.0178340" @default.
- W2620066011 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5441640" @default.
- W2620066011 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28542561" @default.
- W2620066011 hasPublicationYear "2017" @default.
- W2620066011 type Work @default.
- W2620066011 sameAs 2620066011 @default.
- W2620066011 citedByCount "21" @default.
- W2620066011 countsByYear W26200660112017 @default.
- W2620066011 countsByYear W26200660112018 @default.
- W2620066011 countsByYear W26200660112019 @default.
- W2620066011 countsByYear W26200660112020 @default.
- W2620066011 countsByYear W26200660112021 @default.
- W2620066011 countsByYear W26200660112022 @default.
- W2620066011 countsByYear W26200660112023 @default.
- W2620066011 crossrefType "journal-article" @default.
- W2620066011 hasAuthorship W2620066011A5008233606 @default.
- W2620066011 hasAuthorship W2620066011A5027385851 @default.
- W2620066011 hasAuthorship W2620066011A5028469537 @default.
- W2620066011 hasAuthorship W2620066011A5034204040 @default.
- W2620066011 hasAuthorship W2620066011A5035837751 @default.
- W2620066011 hasBestOaLocation W26200660111 @default.
- W2620066011 hasConcept C112672928 @default.
- W2620066011 hasConcept C124490489 @default.
- W2620066011 hasConcept C126322002 @default.
- W2620066011 hasConcept C134018914 @default.
- W2620066011 hasConcept C16685009 @default.
- W2620066011 hasConcept C172680121 @default.
- W2620066011 hasConcept C178790620 @default.
- W2620066011 hasConcept C185592680 @default.
- W2620066011 hasConcept C202751555 @default.
- W2620066011 hasConcept C2776953305 @default.
- W2620066011 hasConcept C2777218350 @default.
- W2620066011 hasConcept C2777411675 @default.
- W2620066011 hasConcept C2778608917 @default.
- W2620066011 hasConcept C2779234561 @default.
- W2620066011 hasConcept C2780394083 @default.