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- W2620093212 abstract "Isolation of Klebsiella pneumoniae resistant mutants against BRL 41897A antibiotics using Tn pho A has yielded 30 mutants which have been characterized. In order to identify whether the mutants are as a result of the insertion (transposition) of Tn pho A, and to count how many copies of Tn pho A are inserted in the each mutant chromosome, several chromosomal DNA of the mutants have been cut with restriction enzymes and analised using Southern blot method and DIG conjugation. The results showed that at least one mutant (KSL19) carried one copy of transposon, whereas at least 2 mutants (KSL38 and KSL52, ) have 2 copies. It is obvious that Tn pho A mutagenesis provides an efficient method of selecting mutants which are defective in genes controlling expression of the outer membrane and the periplasmic membrane proteins. Key words : Resistant K. pneumoniae mutants- Tn pho A mutagenesis – BRL 41897A- Southern Blot." @default.
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- W2620093212 date "2005-10-01" @default.
- W2620093212 modified "2023-10-02" @default.
- W2620093212 title "IDENTIFICATION OF Klebsiella pneumoniae RESISTANT MUTANTS AGAINST BRL 41897A ANTIBIOTIC USING SOUTHERN BLOT ANALYSIS." @default.
- W2620093212 doi "https://doi.org/10.14499/indonesianjpharm0iss0pp185-192" @default.
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