Matches in SemOpenAlex for { <https://semopenalex.org/work/W2620200123> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W2620200123 endingPage "103" @default.
- W2620200123 startingPage "98" @default.
- W2620200123 abstract "Abstract In the present work, an automatic methodology, allowing the evaluation of peroxidase activity, and the comparison of the effect of two substrates in this activity, was developed. Automation was carried out using a miniaturized micro-sequential injection analysis system, usually called μSIA-LOV (Lab-on-valve). This kind of system allows downscaling of current flow injection and sequential injection systems. For the automatic evaluation of peroxidase activity it was selected two different reactions using different substrates - 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate (ABTS) and a mixture of 4-aminoantipyrine and phenol, that were implemented in a μSIA-LOV manifold. The experimental parameters such as reagent ratios and volumes, pH, temperature and reaction time, were optimized and kept constant for the two reactions. The use of these two reactions allowed the applicability of the system in two different ranges of concentrations (up to 0.20 U mL − 1 when it was used ABTS and up to 0.80 U mL − 1 for the reaction with 4-AAP and phenol) with different sensitivities and detection limits of 1.9 × 10 − 4 U mL − 1 and 9.2 × 10 − 3 U mL − 1 (using ABTS and 4-AAP with phenol, respectively). Horseradish, ginger and avocado samples were analyzed proving that the developed methodology constitutes a robust, versatile, rapid and simple alternative for the evaluation of peroxidase activity, adapted to the actual demands of reagents economy and reduced residue production, being in good agreement with the actual recommendations of green chemistry." @default.
- W2620200123 created "2017-06-05" @default.
- W2620200123 creator A5009439913 @default.
- W2620200123 creator A5020281731 @default.
- W2620200123 creator A5024734753 @default.
- W2620200123 creator A5064462111 @default.
- W2620200123 date "2017-09-01" @default.
- W2620200123 modified "2023-09-24" @default.
- W2620200123 title "Automatic evaluation of peroxidase activity using different substrates under a micro sequential injection analysis/lab-on-valve (μSIA-LOV) format" @default.
- W2620200123 cites W1970696999 @default.
- W2620200123 cites W1972754384 @default.
- W2620200123 cites W1994746271 @default.
- W2620200123 cites W2000145794 @default.
- W2620200123 cites W2011725737 @default.
- W2620200123 cites W2033656534 @default.
- W2620200123 cites W2037076214 @default.
- W2620200123 cites W2047445018 @default.
- W2620200123 cites W2050379759 @default.
- W2620200123 cites W2064123588 @default.
- W2620200123 cites W2064781457 @default.
- W2620200123 cites W2067718532 @default.
- W2620200123 cites W2074910695 @default.
- W2620200123 cites W2077426075 @default.
- W2620200123 cites W2095262018 @default.
- W2620200123 cites W2129832192 @default.
- W2620200123 cites W2147194612 @default.
- W2620200123 cites W2152405429 @default.
- W2620200123 cites W2158183161 @default.
- W2620200123 doi "https://doi.org/10.1016/j.microc.2017.05.010" @default.
- W2620200123 hasPublicationYear "2017" @default.
- W2620200123 type Work @default.
- W2620200123 sameAs 2620200123 @default.
- W2620200123 citedByCount "10" @default.
- W2620200123 countsByYear W26202001232018 @default.
- W2620200123 countsByYear W26202001232019 @default.
- W2620200123 countsByYear W26202001232020 @default.
- W2620200123 countsByYear W26202001232022 @default.
- W2620200123 crossrefType "journal-article" @default.
- W2620200123 hasAuthorship W2620200123A5009439913 @default.
- W2620200123 hasAuthorship W2620200123A5020281731 @default.
- W2620200123 hasAuthorship W2620200123A5024734753 @default.
- W2620200123 hasAuthorship W2620200123A5064462111 @default.
- W2620200123 hasConcept C185592680 @default.
- W2620200123 hasConcept C41008148 @default.
- W2620200123 hasConceptScore W2620200123C185592680 @default.
- W2620200123 hasConceptScore W2620200123C41008148 @default.
- W2620200123 hasFunder F4320320300 @default.
- W2620200123 hasFunder F4320334953 @default.
- W2620200123 hasLocation W26202001231 @default.
- W2620200123 hasOpenAccess W2620200123 @default.
- W2620200123 hasPrimaryLocation W26202001231 @default.
- W2620200123 hasRelatedWork W1531601525 @default.
- W2620200123 hasRelatedWork W1990781990 @default.
- W2620200123 hasRelatedWork W2314643286 @default.
- W2620200123 hasRelatedWork W2606768298 @default.
- W2620200123 hasRelatedWork W2606769243 @default.
- W2620200123 hasRelatedWork W2606837276 @default.
- W2620200123 hasRelatedWork W2748952813 @default.
- W2620200123 hasRelatedWork W2899084033 @default.
- W2620200123 hasRelatedWork W2948807893 @default.
- W2620200123 hasRelatedWork W2778153218 @default.
- W2620200123 hasVolume "134" @default.
- W2620200123 isParatext "false" @default.
- W2620200123 isRetracted "false" @default.
- W2620200123 magId "2620200123" @default.
- W2620200123 workType "article" @default.