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• W2620245575 abstract "Abstract Prolyl oligopeptidase (PREP) is conserved in many organisms across life. It is involved in numerous processes including brain function and neuropathology, that require more than its strict proteolytic role. It consists of a seven-bladed β-propeller juxtaposed to a catalytic α/β-hydrolase domain. The conformational dynamics of PREP involved in domain motions and the gating mechanism that allows substrate accessibility remain elusive. Here we used Hydrogen Deuterium eXchange Mass Spectrometry (HDX-MS) to derive the first near-residue resolution analysis of global PREP dynamics in the presence or absence of inhibitor bound in the active site. Clear roles are revealed for parts that would be critical for the activation mechanism. In the free state, the inter-domain interface is loose, providing access to the catalytic site. Inhibitor binding “locks” the two domains together exploiting prominent interactions between the loop of the first β-propeller blade and its proximal helix from the α/β-hydrolase domain. Loop A, thought to drive gating, is partially stabilized but remains flexible and dynamic. These findings provide a conformational guide for further dissection of the gating mechanism of PREP, that would impact drug development. Moreover, they offer a structural framework against which to study proteolysis-independent interactions with disordered proteins like α-synuclein involved in neurodegenerative disease." @default.
• W2620245575 created "2017-06-05" @default.
• W2620245575 creator A5028410215 @default.
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• W2620245575 creator A5039822875 @default.
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• W2620245575 creator A5081260368 @default.
• W2620245575 date "2017-05-26" @default.
• W2620245575 modified "2023-10-01" @default.
• W2620245575 title "Dynamics and ligand-induced conformational changes in human prolyl oligopeptidase analyzed by hydrogen/deuterium exchange mass spectrometry" @default.
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• W2620245575 doi "https://doi.org/10.1038/s41598-017-02550-1" @default.
• W2620245575 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5446394" @default.
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• W2620245575 hasPublicationYear "2017" @default.
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