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- W2620512046 abstract "Abstract Terephthalic acid (TPA) is an important industrial chemical currently produced by energy intensive and potentially hazardous p- xylene ( p X) oxidation process. Here we report the development of metabolically engineered Escherichia coli system for biological transformation of p X into TPA. The engineered E. coli strain harbours a synthetic TPA pathway optimized through manipulation of expression levels of upstream and downstream modules. The upstream pathway converts p X to p -toluic acid ( p TA) and the downstream pathway transforms p TA to TPA. In a two-phase partitioning fermentation, the engineered strain converts 8.8 g p X into 13.3 g TPA, which corresponds to a conversion yield of 96.7 mol%. These results suggest that the E. coli system presented here might be a promising alternative for the large-scale biotechnological production of TPA and lays the foundations for the future development of sustainable approaches for TPA production." @default.
- W2620512046 created "2017-06-05" @default.
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- W2620512046 date "2017-05-31" @default.
- W2620512046 modified "2023-10-07" @default.
- W2620512046 title "Biotransformation of p-xylene into terephthalic acid by engineered Escherichia coli" @default.
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- W2620512046 doi "https://doi.org/10.1038/ncomms15689" @default.
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