Matches in SemOpenAlex for { <https://semopenalex.org/work/W2620680363> ?p ?o ?g. }
- W2620680363 endingPage "27" @default.
- W2620680363 startingPage "16" @default.
- W2620680363 abstract "Development of novel strategies to control fungal plant pathogens requires understanding of their cellular organisation and biology. Live cell imaging of fluorescent organelle markers has provided valuable insight into various aspects of their cell biology, including invasion strategies in plant pathogenic fungi. Here, we introduce a set of 17 vectors that encode fluorescent markers to visualize the plasma membrane, endoplasmic reticulum (ER), chromosomes, the actin cytoskeleton, peroxisomes and autophagosomes in the wheat pathogen Zymoseptoria tritici. We fused either enhanced green-fluorescent protein (eGFP) or a codon-optimised version of GFP (ZtGFP) to homologues of a plasma membrane-located Sso1-like syntaxin, an ER signalling and retention peptide, a histone H1 homologue, the LifeAct actin-binding peptide, a mitochondrial acetyl-CoA dehydrogenase, a peroxisomal import signal and a homologue of the ubiquitin-like autophagosomal protein Atg8. We expressed these markers in wildtype strain IPO323 and confirmed the specificity of these markers by counterstaining or physiological experiments. This new set of molecular tools will help understanding the cell biology of the wheat pathogen Z. tritici." @default.
- W2620680363 created "2017-06-09" @default.
- W2620680363 creator A5039810233 @default.
- W2620680363 creator A5045239920 @default.
- W2620680363 creator A5053477434 @default.
- W2620680363 creator A5071716290 @default.
- W2620680363 date "2017-08-01" @default.
- W2620680363 modified "2023-10-18" @default.
- W2620680363 title "Fluorescent markers of various organelles in the wheat pathogen Zymoseptoria tritici" @default.
- W2620680363 cites W1503394109 @default.
- W2620680363 cites W150631818 @default.
- W2620680363 cites W1968272833 @default.
- W2620680363 cites W1970518843 @default.
- W2620680363 cites W197316475 @default.
- W2620680363 cites W1975468372 @default.
- W2620680363 cites W1977396132 @default.
- W2620680363 cites W1979796171 @default.
- W2620680363 cites W1984221059 @default.
- W2620680363 cites W1990326485 @default.
- W2620680363 cites W2009970692 @default.
- W2620680363 cites W2013673237 @default.
- W2620680363 cites W2013950785 @default.
- W2620680363 cites W2021728645 @default.
- W2620680363 cites W2024713199 @default.
- W2620680363 cites W2029955148 @default.
- W2620680363 cites W2030463413 @default.
- W2620680363 cites W2035940511 @default.
- W2620680363 cites W2040573723 @default.
- W2620680363 cites W2041099435 @default.
- W2620680363 cites W2043018384 @default.
- W2620680363 cites W2045439285 @default.
- W2620680363 cites W2046338633 @default.
- W2620680363 cites W2057764483 @default.
- W2620680363 cites W2058035711 @default.
- W2620680363 cites W2061034298 @default.
- W2620680363 cites W2075951291 @default.
- W2620680363 cites W2076170195 @default.
- W2620680363 cites W2094210517 @default.
- W2620680363 cites W2094667242 @default.
- W2620680363 cites W2097727291 @default.
- W2620680363 cites W2098290877 @default.
- W2620680363 cites W2101441813 @default.
- W2620680363 cites W2102657962 @default.
- W2620680363 cites W2103909509 @default.
- W2620680363 cites W2105914119 @default.
- W2620680363 cites W2113617365 @default.
- W2620680363 cites W2116486678 @default.
- W2620680363 cites W2125775483 @default.
- W2620680363 cites W2127326985 @default.
- W2620680363 cites W2128324727 @default.
- W2620680363 cites W2132632499 @default.
- W2620680363 cites W2134362824 @default.
- W2620680363 cites W2137037625 @default.
- W2620680363 cites W2138585051 @default.
- W2620680363 cites W2139370346 @default.
- W2620680363 cites W2142226006 @default.
- W2620680363 cites W2151177060 @default.
- W2620680363 cites W2152577626 @default.
- W2620680363 cites W2154046894 @default.
- W2620680363 cites W2160276029 @default.
- W2620680363 cites W2162382895 @default.
- W2620680363 cites W2169333903 @default.
- W2620680363 cites W2170566884 @default.
- W2620680363 cites W2185639624 @default.
- W2620680363 cites W2194270206 @default.
- W2620680363 cites W2252770568 @default.
- W2620680363 cites W2306644110 @default.
- W2620680363 cites W2318605407 @default.
- W2620680363 cites W2417901094 @default.
- W2620680363 cites W566471755 @default.
- W2620680363 cites W585575855 @default.
- W2620680363 cites W592697454 @default.
- W2620680363 cites W603318324 @default.
- W2620680363 cites W617996785 @default.
- W2620680363 cites W622892068 @default.
- W2620680363 cites W627727981 @default.
- W2620680363 cites W642760692 @default.
- W2620680363 cites W645058820 @default.
- W2620680363 cites W746467347 @default.
- W2620680363 doi "https://doi.org/10.1016/j.fgb.2017.05.001" @default.
- W2620680363 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5536155" @default.
- W2620680363 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28579390" @default.
- W2620680363 hasPublicationYear "2017" @default.
- W2620680363 type Work @default.
- W2620680363 sameAs 2620680363 @default.
- W2620680363 citedByCount "22" @default.
- W2620680363 countsByYear W26206803632017 @default.
- W2620680363 countsByYear W26206803632019 @default.
- W2620680363 countsByYear W26206803632020 @default.
- W2620680363 countsByYear W26206803632021 @default.
- W2620680363 countsByYear W26206803632022 @default.
- W2620680363 countsByYear W26206803632023 @default.
- W2620680363 crossrefType "journal-article" @default.
- W2620680363 hasAuthorship W2620680363A5039810233 @default.
- W2620680363 hasAuthorship W2620680363A5045239920 @default.
- W2620680363 hasAuthorship W2620680363A5053477434 @default.
- W2620680363 hasAuthorship W2620680363A5071716290 @default.
- W2620680363 hasBestOaLocation W26206803631 @default.