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- W2620694003 abstract "This chapter reviews recent developments in the identification of global splice variant proteins utilizing integrated transcriptomic and proteomic approaches. It describes immunoaffinity mass spectrometry-based proteomic techniques to identify and quantify tissue- and disease-specific expression of protein splicing variants. Several approaches have been employed to interrogate splice variants and compare their expression profiles in normal and disease states. The general approach was to search proteomic data against databases that contain splice variants followed by confirmation of the translation of a spliced sequence by detecting (splice junction) peptide(s) unique to that isoform. While profiling of novel splice variants offers rich information about the disease mechanism and signaling pathway at proteome level, a current issue is the challenge to identify globally low abundance of protein variants due to the huge dynamic range of protein expressions. Targeted in-depth characterization of a particular protein to identify its tissue- and disease-specific splice variant(s) would be an attractive option." @default.
- W2620694003 created "2017-06-09" @default.
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- W2620694003 date "2017-06-02" @default.
- W2620694003 modified "2023-10-17" @default.
- W2620694003 title "Targeting the Right Protein Isoform: Mass Spectrometry‐Based Proteomic Characterization of Alternative Splice Variants" @default.
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- W2620694003 doi "https://doi.org/10.1002/9781119371779.ch6" @default.
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