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• W2620765491 abstract "We previously reported a metabolic engineering strategy to develop an isopropanol producing strain of Cupriavidus necator leading to production of 3.4 g L−1 isopropanol. In order to reach higher titers, isopropanol toxicity to the cells has to be considered. A toxic effect of isopropanol on the growth of C. necator has been indeed observed above a critical value of 15 g L−1. GroESL chaperones were first searched and identified in the genome of C. necator. Native groEL and groES genes from C. necator were over-expressed in a strain deleted for PHA synthesis. We demonstrated that over-expressing groESL genes led to a better tolerance of the strain towards exogenous isopropanol. GroESL genes were then over-expressed within the best engineered isopropanol producing strain. A final isopropanol concentration of 9.8 g L−1 was achieved in fed-batch culture on fructose as the sole carbon source (equivalent to 16 g L−1 after taking into account evaporation). Cell viability was slightly improved by the chaperone over-expression, particularly at the end of the fermentation when the isopropanol concentration was the highest. Moreover, the strain over-expressing the chaperones showed higher enzyme activity levels of the 2 heterologous enzymes (acetoacetate carboxylase and alcohol dehydrogenase) of the isopropanol synthetic operon, translating to a higher specific production rate of isopropanol at the expense of the specific production rate of acetone. Over-expressing the native chaperones led to a 9–18% increase in the isopropanol yield on fructose." @default.
• W2620765491 created "2017-06-09" @default.
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• W2620765491 date "2017-07-01" @default.
• W2620765491 modified "2023-10-15" @default.
• W2620765491 title "Over expression of GroESL in Cupriavidus necator for heterotrophic and autotrophic isopropanol production" @default.
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• W2620765491 doi "https://doi.org/10.1016/j.ymben.2017.05.007" @default.
• W2620765491 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28591561" @default.
• W2620765491 hasPublicationYear "2017" @default.
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