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- W2620984847 abstract "Bovine follicular fluid (FF), secreted by granulosa and theca cells, has been shown to contain biologically active molecules and proteins that may affect follicular growth, oocyte maturation and ovulation. Follicular fluid proteomic profile has a significant impact in the identification of biomarker for oocyte quality estimation and, maybe, for in vitro fertilization success improvement, however is still poorly characterized. We performed a study involving healthy non-lactating Holstein cows to determine the proteome profile of FF in key-stages of the follicular development. Follicles were aspirated in vivo at predeviation, deviation, post deviation and preovulatory stages of the estrous cycle, which were confirmed by measurement of estradiol and progesterone concentrations. Pooled FF samples from each stage were depleted, reduced, alkylated and digested with trypsin. The resulting peptides were labelled with Tandem Mass Tag (TMT-6 plex; Thermo Scientific) according to the protocol supplied by manufacturer. Proteins were identified using LC-MS/MS (Orbitrap Elite ETD) and data analysis was carried on Proteome DiscovererTM 2.1 software. A total of 524 proteins were identified and assigned to a variety of functional processes, including protein binding, enzyme regulator activity, metal ion binding, and catalytic activity. Twenty-two differentially (P<0.05) expressed proteins were found between stages indicating intrafollicular changes, with deviation a critical time point for modulation of follicular growth. For instance, inhibin, follistatin, serglycin, protein HP 20, anti-testosterone antibody, complement C4, fibrinogen, amongst other, were shown to correlate with the first stages of the follicular development. In contrast, alpha-2-macroglobulin, beta-2-glycoprotein, antithrombin-III and immunoglobulins were altered during later stages of the estrous cycle. In conclusion, these differentially expressed proteins provide insights into the size-dependent protein changes in the ovarian follicle microenvironment that may influence follicular function." @default.
- W2620984847 created "2017-06-09" @default.
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- W2620984847 date "2016-10-30" @default.
- W2620984847 modified "2023-10-01" @default.
- W2620984847 title "Follicular fluid proteome profile of dairy cows" @default.
- W2620984847 hasPublicationYear "2016" @default.
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