FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2621423478> ?p ?o ?g. }

• W2621423478 endingPage "613" @default.
• W2621423478 startingPage "613" @default.
• W2621423478 abstract "The objective of this study was to investigate if the number of antral follicles (AF) is correlated with oocyte competence. Ovaries (n = 712) from Nelore cows (n = 356) were obtained at the slaughterhouse and divided in groups according to the number of antral follicles. The mean quantity of follicles was 61.14 ± 30.43 per cow. The experimental groups were defined as: females with ≤ 31 AF (mean number – SD); 46-76 AF (mean number ± ½ SD); and ≥ 92 AF (mean number + SD). Cumulus-oocyte complexes (COCs) were collected from each group, matured, fertilized and cultured in vitro to assess developmental competence to the blastocyst stage. COCs (n = 180) were used to quantify mRNA expression by qRT-PCR in 3 replicates. Total RNA was extracted from granulosa and cumulus cells using Trizol. RNA purity and quantity were determined using a Nano-Drop spectrophotometer, and then 200 ng total RNA per sample was reverse transcribed using the iScript cDNA synthesis kit (BioRad, ON, Canada). Quantitative Real time PCR was performed using a CFX384TM Real-Time System (BioRad) and iQ SYBR Green Supermix (BioRad). mRNA abundance of tested genes was normalized to the average of the internal control genes RP18S and Cyclophilin. Data were submitted to ANOVA and the averages compared by Tukey’s HSD test. Embryo development data were analyzed by logistic regression and differences were considered statistically significant if P < 0.05. There was no difference between groups for cleavage and development to the blastocyst stage. In cumulus cells, samples from cows with ≤ 31 AF had higher mRNA abundance of genes involved in meiosis resumption (NPR-2 and NPR-3), estradiol biosynthesis (CYP19A1), cumulus cells expansion (FGF10), and transcription factors that regulate oocyte maturation and cell proliferation (STAT3 and FOXO3a). There was no difference in mRNA abundance of STAR (involved in steroidogenesis), NPR-1, PGRMC1, PGR (involved in meiosis resumption), LIFRα (transcription factor that regulate oocyte maturation and cell proliferation), FGF2Rb, FSHR, BMPR2, AMH, AMHR2 and TGFsR1 (involved in cumulus cells expansion) in cumulus cells. In granulosa cells, NPR-1 mRNA levels (involved in meiosis resumption) were higher in the group with ≥ 92 AF. Findings from this study revealed molecular differences related to meiosis resumption, synthesis of estradiol, cumulus cells expansion, oocyte maturation and cell proliferation in cumulus cells of Nelore cows of low vs. high number of AF." @default.
• W2621423478 created "2017-06-15" @default.
• W2621423478 creator A5001628972 @default.
• W2621423478 creator A5003942836 @default.
• W2621423478 creator A5003979944 @default.
• W2621423478 creator A5010403312 @default.
• W2621423478 creator A5046863088 @default.
• W2621423478 creator A5050486589 @default.
• W2621423478 creator A5053692069 @default.
• W2621423478 creator A5090353180 @default.
• W2621423478 date "2016-03-01" @default.
• W2621423478 modified "2023-09-26" @default.
• W2621423478 title "Expression of genes involved in oocyte competence is higher in cumulus cells of Nelore cows with low compared to high number of antral follicle" @default.
• W2621423478 hasPublicationYear "2016" @default.
• W2621423478 type Work @default.
• W2621423478 sameAs 2621423478 @default.
• W2621423478 citedByCount "0" @default.
• W2621423478 crossrefType "journal-article" @default.
• W2621423478 hasAuthorship W2621423478A5001628972 @default.
• W2621423478 hasAuthorship W2621423478A5003942836 @default.
• W2621423478 hasAuthorship W2621423478A5003979944 @default.
• W2621423478 hasAuthorship W2621423478A5010403312 @default.
• W2621423478 hasAuthorship W2621423478A5046863088 @default.
• W2621423478 hasAuthorship W2621423478A5050486589 @default.
• W2621423478 hasAuthorship W2621423478A5053692069 @default.
• W2621423478 hasAuthorship W2621423478A5090353180 @default.
• W2621423478 hasConcept C126322002 @default.
• W2621423478 hasConcept C134018914 @default.
• W2621423478 hasConcept C142773365 @default.
• W2621423478 hasConcept C16685009 @default.
• W2621423478 hasConcept C187785154 @default.
• W2621423478 hasConcept C196843134 @default.
• W2621423478 hasConcept C2776690073 @default.
• W2621423478 hasConcept C2778177303 @default.
• W2621423478 hasConcept C54355233 @default.
• W2621423478 hasConcept C71924100 @default.
• W2621423478 hasConcept C86803240 @default.
• W2621423478 hasConcept C87073359 @default.
• W2621423478 hasConcept C99476002 @default.
• W2621423478 hasConceptScore W2621423478C126322002 @default.
• W2621423478 hasConceptScore W2621423478C134018914 @default.
• W2621423478 hasConceptScore W2621423478C142773365 @default.
• W2621423478 hasConceptScore W2621423478C16685009 @default.
• W2621423478 hasConceptScore W2621423478C187785154 @default.
• W2621423478 hasConceptScore W2621423478C196843134 @default.
• W2621423478 hasConceptScore W2621423478C2776690073 @default.
• W2621423478 hasConceptScore W2621423478C2778177303 @default.
• W2621423478 hasConceptScore W2621423478C54355233 @default.
• W2621423478 hasConceptScore W2621423478C71924100 @default.
• W2621423478 hasConceptScore W2621423478C86803240 @default.
• W2621423478 hasConceptScore W2621423478C87073359 @default.
• W2621423478 hasConceptScore W2621423478C99476002 @default.
• W2621423478 hasIssue "3" @default.
• W2621423478 hasOpenAccess W2621423478 @default.
• W2621423478 hasRelatedWork W1986798664 @default.
• W2621423478 hasRelatedWork W1990492035 @default.
• W2621423478 hasRelatedWork W1993552330 @default.
• W2621423478 hasRelatedWork W2037478111 @default.
• W2621423478 hasRelatedWork W2043322857 @default.
• W2621423478 hasRelatedWork W2065967978 @default.
• W2621423478 hasRelatedWork W2114032346 @default.
• W2621423478 hasRelatedWork W2126003141 @default.
• W2621423478 hasRelatedWork W2140354831 @default.
• W2621423478 hasRelatedWork W2161550987 @default.
• W2621423478 hasRelatedWork W2617170518 @default.
• W2621423478 hasRelatedWork W2647951630 @default.
• W2621423478 hasRelatedWork W2725961743 @default.
• W2621423478 hasRelatedWork W3159627507 @default.
• W2621423478 hasRelatedWork W2012994599 @default.
• W2621423478 hasRelatedWork W2617095720 @default.
• W2621423478 hasRelatedWork W2622585914 @default.
• W2621423478 hasRelatedWork W2622960171 @default.
• W2621423478 hasRelatedWork W2625645815 @default.
• W2621423478 hasRelatedWork W2791847364 @default.
• W2621423478 hasVolume "13" @default.
• W2621423478 isParatext "false" @default.
• W2621423478 isRetracted "false" @default.
• W2621423478 magId "2621423478" @default.
• W2621423478 workType "article" @default.