FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2622097680> ?p ?o ?g. }

• W2622097680 abstract "Abstract Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. However, understanding the roles and regulations of site-specific glycosylation events remains a significant challenge due to several technological limitations. These limitations include a lack of available tools for biochemical characterization of enzymes involved in glycosylation. A particular challenge is the synthesis of oligosaccharyltransferases (OSTs), which catalyze the attachment of glycans to specific amino acid residues in target proteins. The difficulty arises from the fact that canonical OSTs are large (>70 kDa) and possess multiple transmembrane helices, making them difficult to overexpress in living cells. Here, we address this challenge by establishing a bacterial cell-free protein synthesis platform that enables rapid production of a variety of OSTs in their active conformations. Specifically, by using lipid nanodiscs as cellular membrane mimics, we obtained yields of up to 440 µg/mL for the single-subunit OST enzyme, ‘Protein glycosylation B’ (PglB) from Campylobacter jejuni , as well as for three additional PglB homologs from Campylobacter coli, Campylobacter lari , and Desulfovibrio gigas . Importantly, all of these enzymes catalyzed N -glycosylation reactions in vitro with no purification or processing needed. Furthermore, we demonstrate the ability of cell-free synthesized OSTs to glycosylate multiple target proteins with varying N -glycosylation acceptor sequons. We anticipate that this broadly applicable production method will advance glycoengineering efforts by enabling preparative expression of membrane-embedded OSTs from all kingdoms of life." @default.
• W2622097680 created "2017-06-15" @default.
• W2622097680 creator A5000309167 @default.
• W2622097680 creator A5025044375 @default.
• W2622097680 creator A5028438229 @default.
• W2622097680 creator A5036419983 @default.
• W2622097680 creator A5037878850 @default.
• W2622097680 creator A5040713951 @default.
• W2622097680 creator A5056155059 @default.
• W2622097680 creator A5059800162 @default.
• W2622097680 creator A5081822200 @default.
• W2622097680 date "2017-06-05" @default.
• W2622097680 modified "2023-10-05" @default.
• W2622097680 title "A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases" @default.
• W2622097680 cites W1554644696 @default.
• W2622097680 cites W1587590458 @default.
• W2622097680 cites W1754512095 @default.
• W2622097680 cites W1779999055 @default.
• W2622097680 cites W1807215826 @default.
• W2622097680 cites W1939818047 @default.
• W2622097680 cites W1964462261 @default.
• W2622097680 cites W1968701309 @default.
• W2622097680 cites W1970482223 @default.
• W2622097680 cites W1975434365 @default.
• W2622097680 cites W1981070004 @default.
• W2622097680 cites W1987145185 @default.
• W2622097680 cites W1987804131 @default.
• W2622097680 cites W1987959660 @default.
• W2622097680 cites W1990133186 @default.
• W2622097680 cites W1990530150 @default.
• W2622097680 cites W1991984239 @default.
• W2622097680 cites W1996201718 @default.
• W2622097680 cites W1997394451 @default.
• W2622097680 cites W2000823773 @default.
• W2622097680 cites W2002722201 @default.
• W2622097680 cites W2004125187 @default.
• W2622097680 cites W2005574282 @default.
• W2622097680 cites W2008686474 @default.
• W2622097680 cites W2012114611 @default.
• W2622097680 cites W2028267773 @default.
• W2622097680 cites W2035176534 @default.
• W2622097680 cites W2035569341 @default.
• W2622097680 cites W2045151072 @default.
• W2622097680 cites W2052060414 @default.
• W2622097680 cites W2057487309 @default.
• W2622097680 cites W2071794645 @default.
• W2622097680 cites W2075797628 @default.
• W2622097680 cites W2076117904 @default.
• W2622097680 cites W2078703243 @default.
• W2622097680 cites W2083780169 @default.
• W2622097680 cites W2086334871 @default.
• W2622097680 cites W2087993212 @default.
• W2622097680 cites W2088221752 @default.
• W2622097680 cites W2089832684 @default.
• W2622097680 cites W2102073086 @default.
• W2622097680 cites W2103137962 @default.
• W2622097680 cites W2105385262 @default.
• W2622097680 cites W2108270497 @default.
• W2622097680 cites W2117210366 @default.
• W2622097680 cites W2122559203 @default.
• W2622097680 cites W2123703307 @default.
• W2622097680 cites W2129232972 @default.
• W2622097680 cites W2139398575 @default.
• W2622097680 cites W2145832204 @default.
• W2622097680 cites W2146561267 @default.
• W2622097680 cites W2149369491 @default.
• W2622097680 cites W2150764734 @default.
• W2622097680 cites W2154719226 @default.
• W2622097680 cites W2155372226 @default.
• W2622097680 cites W2155909615 @default.
• W2622097680 cites W2161171034 @default.
• W2622097680 cites W2167215594 @default.
• W2622097680 cites W2177943983 @default.
• W2622097680 cites W2277374379 @default.
• W2622097680 cites W2302411709 @default.
• W2622097680 cites W2304171844 @default.
• W2622097680 cites W2346028445 @default.
• W2622097680 cites W2505971024 @default.
• W2622097680 cites W2511075865 @default.
• W2622097680 cites W2566131830 @default.
• W2622097680 cites W2587450560 @default.
• W2622097680 doi "https://doi.org/10.1101/145227" @default.
• W2622097680 hasPublicationYear "2017" @default.
• W2622097680 type Work @default.
• W2622097680 sameAs 2622097680 @default.
• W2622097680 citedByCount "3" @default.
• W2622097680 countsByYear W26220976802017 @default.
• W2622097680 countsByYear W26220976802019 @default.
• W2622097680 crossrefType "posted-content" @default.
• W2622097680 hasAuthorship W2622097680A5000309167 @default.
• W2622097680 hasAuthorship W2622097680A5025044375 @default.
• W2622097680 hasAuthorship W2622097680A5028438229 @default.
• W2622097680 hasAuthorship W2622097680A5036419983 @default.
• W2622097680 hasAuthorship W2622097680A5037878850 @default.
• W2622097680 hasAuthorship W2622097680A5040713951 @default.
• W2622097680 hasAuthorship W2622097680A5056155059 @default.
• W2622097680 hasAuthorship W2622097680A5059800162 @default.
• W2622097680 hasAuthorship W2622097680A5081822200 @default.
• W2622097680 hasBestOaLocation W26220976801 @default.
• W2622097680 hasConcept C108625454 @default.

• next