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• W2622153969 abstract "Background: Contrast media is widely used in clinical diagnostic and interventional procedures, but may cause damage to the kidney, that is, contrast-induced nephropathy. This study was to establish a dual-label time-resolved fluorescence immunoassay (TRFIA) for the simultaneous determination of renal function markers cystatin-C (Cys-C) and β2-microglobulin (β2-MG) for the early diagnosis and follow-up surveillance of contrast-induced nephropathy.Methods: A sandwich immunoassay was used to detect the concentration of Cys-C, and the competitive immunoassay was used to detect the concentration of β2-MG in 50 samples of urine. The performance of this dual-label TRFIA was evaluated and compared with commercial assays.Results: The sensitivity for Cys-C detection was 1.26 ng/ml, the average recovery was 99.36%; The sensitivity for β2-MG detection was 2.13 ng/ml, the average recovery was 100.18%. Bland–Altman analysis showed that the dual-label TRFIA method and the commercial kits had a good agreement, suggesting they can be used interchangeably in clinical urine analysis.Conclusion: The present dual-label TRFIA has high sensitivity, specificity and accuracy in clinical sample analysis. This method can be used for the early diagnosis and follow-up surveillance of the contrast-induced nephropathy." @default.
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• W2622153969 date "2017-07-21" @default.
• W2622153969 modified "2023-10-18" @default.
• W2622153969 title "A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of cystatin C and β₂-microglobulin in urine" @default.
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• W2622153969 doi "https://doi.org/10.1080/09674845.2017.1334740" @default.
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