Matches in SemOpenAlex for { <https://semopenalex.org/work/W2622272571> ?p ?o ?g. }
- W2622272571 abstract "Neurosteroid dehydroepiandrosterone (DHEA) has been reported to exert a potent neuroprotective effect against glutamate-induced excitotoxicity. However, the underlying mechanism remains to be elucidated. One of the possible mechanisms may be an involvement of astrocytic glutamate transporter subtype-1 (GLT-1) that can quickly clear spilled glutamate at the synapse to prevent excitotoxicity. To examine the effect of DHEA on GLT-1 activity, we measured synaptically induced glial depolarization (SIGD) in the dentate gyrus (DG) of adult rats by applying an optical recording technique to the hippocampal slices stained with voltage-sensitive dye RH155. Bath-application of DHEA for 10 min dose-dependently increased SIGD without changing presynaptic glutamate releases, which was sensitive to the GLT-1 blocker DHK. Patch-clamp recordings in astrocytes showed that an application of 50 μM DHEA increased glutamate-evoked inward currents (Iglu) by approximately 1.5-fold, which was dependent on the GLT-1 activity. In addition, the level of biotinylated GLT-1 protein in the surface of astrocytes was significantly elevated by DHEA. The DHEA-increased SIGD, Iglu, and GLT-1 translocation to the cell surface were blocked by the σ1 R antagonist NE100 and mimicked by the σ1 R agonist PRE084. DHEA elevated the phosphorylation level of PKC in a σ1 R-dependent manner. Furthermore, the PKC inhibitor chelerythrine could prevent the DHEA-increased SIGD, Iglu, and GLT-1 translocation. Collectively, present results suggest that DHEA enhances the activity and translocation to cell surface of astrocytic GLT-1 mainly via σ1 R-mediated PKC cascade." @default.
- W2622272571 created "2017-06-15" @default.
- W2622272571 creator A5014952697 @default.
- W2622272571 creator A5017384752 @default.
- W2622272571 creator A5031653114 @default.
- W2622272571 creator A5041791168 @default.
- W2622272571 creator A5047717885 @default.
- W2622272571 creator A5064068441 @default.
- W2622272571 creator A5081093203 @default.
- W2622272571 date "2017-06-05" @default.
- W2622272571 modified "2023-10-16" @default.
- W2622272571 title "Neurosteroid dehydroepiandrosterone enhances activity and trafficking of astrocytic GLT-1viaσ1receptor-mediated PKC activation in the hippocampal dentate gyrus of rats" @default.
- W2622272571 cites W1922739097 @default.
- W2622272571 cites W1956166033 @default.
- W2622272571 cites W1963565483 @default.
- W2622272571 cites W1965902234 @default.
- W2622272571 cites W1966846060 @default.
- W2622272571 cites W1970705129 @default.
- W2622272571 cites W1971279713 @default.
- W2622272571 cites W1979889191 @default.
- W2622272571 cites W1981837661 @default.
- W2622272571 cites W1989888504 @default.
- W2622272571 cites W1994463266 @default.
- W2622272571 cites W1994514659 @default.
- W2622272571 cites W1994745690 @default.
- W2622272571 cites W2001188459 @default.
- W2622272571 cites W2001674635 @default.
- W2622272571 cites W2004251958 @default.
- W2622272571 cites W2007631668 @default.
- W2622272571 cites W2010078015 @default.
- W2622272571 cites W2016839788 @default.
- W2622272571 cites W2028279469 @default.
- W2622272571 cites W2028309001 @default.
- W2622272571 cites W2031965957 @default.
- W2622272571 cites W2035647324 @default.
- W2622272571 cites W2035673694 @default.
- W2622272571 cites W2037626982 @default.
- W2622272571 cites W2038859198 @default.
- W2622272571 cites W2041389478 @default.
- W2622272571 cites W2046349516 @default.
- W2622272571 cites W2054480718 @default.
- W2622272571 cites W2055244899 @default.
- W2622272571 cites W2057707791 @default.
- W2622272571 cites W2058608288 @default.
- W2622272571 cites W2061974563 @default.
- W2622272571 cites W2072594486 @default.
- W2622272571 cites W2073789713 @default.
- W2622272571 cites W2074054937 @default.
- W2622272571 cites W2074822339 @default.
- W2622272571 cites W2080301005 @default.
- W2622272571 cites W2082450845 @default.
- W2622272571 cites W2095002447 @default.
- W2622272571 cites W2099209146 @default.
- W2622272571 cites W2101092318 @default.
- W2622272571 cites W2109078104 @default.
- W2622272571 cites W2117226055 @default.
- W2622272571 cites W2122860707 @default.
- W2622272571 cites W2126033464 @default.
- W2622272571 cites W2128744963 @default.
- W2622272571 cites W2132504992 @default.
- W2622272571 cites W2135573968 @default.
- W2622272571 cites W2144341669 @default.
- W2622272571 cites W2154115500 @default.
- W2622272571 cites W2154267426 @default.
- W2622272571 cites W2158198173 @default.
- W2622272571 cites W2161452179 @default.
- W2622272571 cites W2167357910 @default.
- W2622272571 cites W2410122486 @default.
- W2622272571 cites W2414750682 @default.
- W2622272571 doi "https://doi.org/10.1002/glia.23175" @default.
- W2622272571 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28581152" @default.
- W2622272571 hasPublicationYear "2017" @default.
- W2622272571 type Work @default.
- W2622272571 sameAs 2622272571 @default.
- W2622272571 citedByCount "8" @default.
- W2622272571 countsByYear W26222725712018 @default.
- W2622272571 countsByYear W26222725712019 @default.
- W2622272571 countsByYear W26222725712020 @default.
- W2622272571 countsByYear W26222725712021 @default.
- W2622272571 crossrefType "journal-article" @default.
- W2622272571 hasAuthorship W2622272571A5014952697 @default.
- W2622272571 hasAuthorship W2622272571A5017384752 @default.
- W2622272571 hasAuthorship W2622272571A5031653114 @default.
- W2622272571 hasAuthorship W2622272571A5041791168 @default.
- W2622272571 hasAuthorship W2622272571A5047717885 @default.
- W2622272571 hasAuthorship W2622272571A5064068441 @default.
- W2622272571 hasAuthorship W2622272571A5081093203 @default.
- W2622272571 hasBestOaLocation W26222725711 @default.
- W2622272571 hasConcept C11960822 @default.
- W2622272571 hasConcept C126322002 @default.
- W2622272571 hasConcept C134018914 @default.
- W2622272571 hasConcept C148762608 @default.
- W2622272571 hasConcept C168258287 @default.
- W2622272571 hasConcept C169760540 @default.
- W2622272571 hasConcept C170493617 @default.
- W2622272571 hasConcept C195794163 @default.
- W2622272571 hasConcept C2776108454 @default.
- W2622272571 hasConcept C2776464000 @default.
- W2622272571 hasConcept C2776992908 @default.
- W2622272571 hasConcept C2777911890 @default.