FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2622275280> ?p ?o ?g. }

• W2622275280 abstract "G-Protein-Coupled Receptors (GPCRs) are a large family of transmembrane receptors that play critical roles in normal cellular physiology and constitute a major pharmacological target for multiple indications, including analgesia, blood pressure regulation, and the treatment of psychiatric disease. Upon ligand binding, GPCRs catalyze the activation of intracellular G-proteins by stimulating the incorporation of guanosine triphosphate (GTP). Activated G-proteins then stimulate signaling pathways that elicit cellular responses. GPCR signaling can be monitored by measuring the incorporation of a radiolabeled and non-hydrolyzable form of GTP, [35S]guanosine-5'-O-(3-thio)triphosphate ([35S]GTPγS), into G-proteins. Unlike other methods that assess more downstream signaling processes, [35S]GTPγS binding measures a proximal event in GPCR signaling and, importantly, can distinguish agonists, antagonists, and inverse agonists. The present protocol outlines a sensitive and specific method for studying GPCR signaling using crude membrane preparations of an archetypal GPCR, the µ-opioid receptor (MOR1). Although alternative approaches to fractionate cells and tissues exist, many are cost-prohibitive, tedious, and/or require non-standard laboratory equipment. The present method provides a simple procedure that enriches functional crude membranes. After isolating MOR1, various pharmacological properties of its agonist, [D-Ala, N-MePhe, Gly-ol]-enkephalin (DAMGO), and antagonist, naloxone, were determined." @default.
• W2622275280 created "2017-06-15" @default.
• W2622275280 creator A5007817146 @default.
• W2622275280 creator A5020208893 @default.
• W2622275280 creator A5073085630 @default.
• W2622275280 creator A5081624863 @default.
• W2622275280 creator A5087008106 @default.
• W2622275280 date "2017-06-09" @default.
• W2622275280 modified "2023-09-25" @default.
• W2622275280 title "Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding" @default.
• W2622275280 cites W1486205509 @default.
• W2622275280 cites W1497141468 @default.
• W2622275280 cites W1515847803 @default.
• W2622275280 cites W1968682237 @default.
• W2622275280 cites W1977676788 @default.
• W2622275280 cites W1978310995 @default.
• W2622275280 cites W1984212762 @default.
• W2622275280 cites W1993755473 @default.
• W2622275280 cites W2006913937 @default.
• W2622275280 cites W2018483093 @default.
• W2622275280 cites W2023634139 @default.
• W2622275280 cites W2035774084 @default.
• W2622275280 cites W2044147064 @default.
• W2622275280 cites W2056502744 @default.
• W2622275280 cites W2059501084 @default.
• W2622275280 cites W2060207881 @default.
• W2622275280 cites W2074631079 @default.
• W2622275280 cites W2085681533 @default.
• W2622275280 cites W2097186487 @default.
• W2622275280 cites W2149963584 @default.
• W2622275280 cites W2160536323 @default.
• W2622275280 doi "https://doi.org/10.3791/55561" @default.
• W2622275280 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5608332" @default.
• W2622275280 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28654029" @default.
• W2622275280 hasPublicationYear "2017" @default.
• W2622275280 type Work @default.
• W2622275280 sameAs 2622275280 @default.
• W2622275280 citedByCount "5" @default.
• W2622275280 countsByYear W26222752802019 @default.
• W2622275280 countsByYear W26222752802020 @default.
• W2622275280 countsByYear W26222752802022 @default.
• W2622275280 countsByYear W26222752802023 @default.
• W2622275280 crossrefType "journal-article" @default.
• W2622275280 hasAuthorship W2622275280A5007817146 @default.
• W2622275280 hasAuthorship W2622275280A5020208893 @default.
• W2622275280 hasAuthorship W2622275280A5073085630 @default.
• W2622275280 hasAuthorship W2622275280A5081624863 @default.
• W2622275280 hasAuthorship W2622275280A5087008106 @default.
• W2622275280 hasBestOaLocation W26222752802 @default.
• W2622275280 hasConcept C118716 @default.
• W2622275280 hasConcept C135285700 @default.
• W2622275280 hasConcept C150555746 @default.
• W2622275280 hasConcept C170493617 @default.
• W2622275280 hasConcept C181199279 @default.
• W2622275280 hasConcept C185592680 @default.
• W2622275280 hasConcept C2776638253 @default.
• W2622275280 hasConcept C2777803847 @default.
• W2622275280 hasConcept C2777995097 @default.
• W2622275280 hasConcept C2778938600 @default.
• W2622275280 hasConcept C2781063702 @default.
• W2622275280 hasConcept C55493867 @default.
• W2622275280 hasConcept C62478195 @default.
• W2622275280 hasConcept C80631254 @default.
• W2622275280 hasConcept C86803240 @default.
• W2622275280 hasConcept C89951826 @default.
• W2622275280 hasConcept C95444343 @default.
• W2622275280 hasConceptScore W2622275280C118716 @default.
• W2622275280 hasConceptScore W2622275280C135285700 @default.
• W2622275280 hasConceptScore W2622275280C150555746 @default.
• W2622275280 hasConceptScore W2622275280C170493617 @default.
• W2622275280 hasConceptScore W2622275280C181199279 @default.
• W2622275280 hasConceptScore W2622275280C185592680 @default.
• W2622275280 hasConceptScore W2622275280C2776638253 @default.
• W2622275280 hasConceptScore W2622275280C2777803847 @default.
• W2622275280 hasConceptScore W2622275280C2777995097 @default.
• W2622275280 hasConceptScore W2622275280C2778938600 @default.
• W2622275280 hasConceptScore W2622275280C2781063702 @default.
• W2622275280 hasConceptScore W2622275280C55493867 @default.
• W2622275280 hasConceptScore W2622275280C62478195 @default.
• W2622275280 hasConceptScore W2622275280C80631254 @default.
• W2622275280 hasConceptScore W2622275280C86803240 @default.
• W2622275280 hasConceptScore W2622275280C89951826 @default.
• W2622275280 hasConceptScore W2622275280C95444343 @default.
• W2622275280 hasIssue "124" @default.
• W2622275280 hasLocation W26222752801 @default.
• W2622275280 hasLocation W26222752802 @default.
• W2622275280 hasLocation W26222752803 @default.
• W2622275280 hasLocation W26222752804 @default.
• W2622275280 hasOpenAccess W2622275280 @default.
• W2622275280 hasPrimaryLocation W26222752801 @default.
• W2622275280 hasRelatedWork W1918129895 @default.
• W2622275280 hasRelatedWork W2044584217 @default.
• W2622275280 hasRelatedWork W2093745563 @default.
• W2622275280 hasRelatedWork W2238057263 @default.
• W2622275280 hasRelatedWork W2408271361 @default.
• W2622275280 hasRelatedWork W2415846341 @default.
• W2622275280 hasRelatedWork W2466382835 @default.
• W2622275280 hasRelatedWork W2622275280 @default.
• W2622275280 hasRelatedWork W4229622269 @default.
• W2622275280 hasRelatedWork W4290207872 @default.

• next