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- W2622960368 abstract "Aim: To design a highly specific and sensitive multiplex real-time PCR assay for the differentiation of the pathogen Haemophilus influenzae from its nonpathogenic near-neighbor Haemophilus haemolyticus. Materials & methods: A comparison of 380 Haemophilus spp. genomes was used to identify loci specific for each species. Novel PCR assays targeting H. haemolyticus (hypD) and H. influenzae (siaT) were designed. Results & discussion: PCR screening across 143 isolates demonstrated 100% specificity for hypD and siaT. These two assays were multiplexed with the recently described fucP assay for further differentiation among H. influenzae. Conclusion: The triplex assay provides rapid, unambiguous, sensitive and highly specific genotyping results for the simultaneous detection of hypD and siaT, including fucose-positive H. influenzae (fucP), in a single PCR." @default.
- W2622960368 created "2017-06-15" @default.
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- W2622960368 date "2017-06-01" @default.
- W2622960368 modified "2023-10-16" @default.
- W2622960368 title "Simultaneous identification of <i>Haemophilus influenzae</i> and <i>Haemophilus haemolyticus</i> using real-time PCR" @default.
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- W2622960368 doi "https://doi.org/10.2217/fmb-2016-0215" @default.
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